cfDNA hydroxymethylcytosine profiling for detection metastasis and recurrence of Esophageal Squamous Cell Carcinoma.

Background: A blood-based approach to monitor metastasis and recurrence of esophageal squamous cell carcinoma (ESCC) remains undeveloped. This study aimed to establish a dependable model utilizing cfDNA 5-hydroxymethylcytosines (5hmC) signatures to detect these conditions in ESCC.

Methods: The 5hmC-Seal technique was employed to generate comprehensive 5hmC profiles from the plasma cell-free DNA (cfDNA) of 122 ESCC patients, classified into 72 with metastasis, 50 without metastasis, 30 with recurrence, and 92 without recurrence.

Measurement of Very-High-Energy Diffuse Gamma-Ray Emissions from the Galactic Plane with LHAASO-WCDA.

The diffuse Galactic gamma-ray emission is a very important tool used to study the propagation and interaction of cosmic rays in the Milky Way. In this Letter, we report the measurements of the diffuse emission from the Galactic plane-covering Galactic longitudes from 15° to 235° and latitudes from -5° to +5°, in an energy range of 1 to 25 TeV-made with the Water Cherenkov Detector Array (WCDA) of the Large High Altitude Air Shower Observatory. After the sky regions of known sources are masked, the diffuse emission is detected with 24.

Debate,Dilemma,and Solution of Living Will.

A living will is a record of the patient's willingness to choose the medical care plan at the critical or dying stage when the patient is conscious.Due to the influences of traditional ethical ideas,policies,laws,medical service supply and other factors,the social implementation of living wills face great obstacles.Therefore,we should strengthen the education of citizens' view of life and death,improve the specific operation procedures of living wills,and establish a national security system for palliative care,so as to better promote the development of hospice care in China.

Effects of soy protein isolates and transglutaminase addition on the structure, physicochemical properties, and oil absorption of wheat flour.

The effects of soy protein isolate (SPI) addition and transglutaminase (TG) treatment (SPI-TG) on the microstructure, physicochemical properties, and oil absorption of wheat flour were studied. After the SPI-TG treatment, the disulfide bonds increased to 1.9657 μmol/g.

DHS regulates leaf senescence by modulating gene translation in .

The biochemical and transcriptional regulatory mechanisms of chlorophyll metabolism have been extensively studied, but the translational regulatory mechanisms remain poorly understood. In this study, we found that Nt DHS1 deficiency in N. tabacum resulted in smaller leaves and increased leaf chlorophyll content.

A dataset of single-cell transcriptomic atlas of Bama pig and potential marker genes across seven tissues.

The use of single-cell sequencing technology for single-cell transcriptomics studies in pigs is expanding progressively. However, the comprehensive classification of cell types across different anatomical tissues and organs of pig in multiple datasets remains relatively limited. This study employs single-cell and single-nucleus sequencing technologies in Bama pig to identify unique marker genes and their corresponding transcriptomic profiles across diverse cell types in various anatomical tissues and organs, including subcutaneous fat, visceral fat, psoas major muscle, liver, spleen, lung, and kidney.

Manipulating Interfacial Stability via Preferential Absorption for Highly Stable and Safe 4.6 V LiCoO Cathode.

Elevating the upper cutoff voltage to 4.6 V could effectively increase the reversible capacity of LiCoO (LCO) cathode, whereas the irreversible structural transition, unstable electrode/electrolyte interface and potentially induced safety hazards severely hinder its industrial application. Building a robust cathode/electrolyte interface film by electrolyte engineering is one of the efficient approaches to boost the performance of high-voltage LCO (HV-LCO); however, the elusive interfacial chemistry poses substantial challenges to the rational design of highly compatible electrolytes.

Observer-Based Decentralized Adaptive Control of Interconnected Nonlinear Systems With Output/Input Triggering.

In this article, a double-channel event-triggered control method is developed for nonlinear uncertain interconnected systems using backstepping techniques, which introduces event-triggering mechanisms at both the sensor and controller sides. Using event-triggering mechanism at the sensor side presents a challenge to the backstepping control design as the discontinuous state/output signals received at the controller side result in nondifferentiable virtual control signals. This challenge becomes more pronounced when considering more general types of event-triggering mechanisms.

FK506-binding proteins: Emerging target and therapeutic opportunity in multiple tumors.

The FK506-binding protein (FKBP) family plays a key role in a variety of tumors and is involved in the regulation of important signaling pathways including AKT, NF-κB and p53, which affects cell proliferation, migration, and multiple cell death modes. Here, we summarize the findings that different FKBP family members exhibit dual functions of promoting or inhibiting tumorigenesis in different types of tumors. The expression levels of FKBP family members are closely related to the prognosis of patients, thus might be used as potential diagnostic and prognostic biomarkers.

A label-free colorimetric assay for sensitive screening of T4 polynucleotide kinase activity and inhibition based on enzyme-aided cyclic strand displacement amplification and PNA-gold nanoparticle platform.

The phosphorylation of nucleic acids mediated by 5'-polynucleotide kinase (PNK) exerts a crucial regulatory function in a wide range of significant cellular activities. Nevertheless, the current approaches for detecting PNK require expensive labeled probes and complex instrumentation, making it impossible to achieve real-time, on-site, and rapid analysis. Here, we take T4 PNK as a model and establish a novel colorimetric strategy for the detection of PNK activity and its inhibition by means of a coupled enzyme-assisted cyclic strand displacement amplification (SDA) and peptide nucleic acid (PNA)-gold nanoparticle (AuNP) based platform.

Early caffeine therapy decreases bronchopulmonary dysplasia but might increase mortality in preterm infants? a systematic review and meta-analysis.

Objectives: To assess the effectiveness of early vs. late caffeine therapy for bronchopulmonary dysplasia (BPD) in infants.

Methods: PubMed, Embase, Web of Science, and Cochrane databases were searched up to October 2024.

Two-dimensional conjugated metal-organic frameworks for electrochemical energy conversion and storage.

Effective electrocatalysts and electrodes are the core components of energy conversion and storage systems for sustainable carbon and nitrogen cycles to achieve a carbon-neutral economy. Two-dimensional conjugated metal-organic frameworks (2D c-MOFs) have emerged as multifunctional materials for electrochemical applications benefiting from their similarity to graphene with remarkable conductivity, abundant active sites, devisable components, and well-defined crystalline structures. In this review, the structural design strategies to establish active components with a maximum degree through redox-active ligand assembly in 2D c-MOFs are briefly summarized.

Comparison of Patellar Tracking Following Kinematic Alignment Versus Mechanical Alignment Total Knee Arthroplasty via the Mini-Subvastus Approach.

Objectives: Different alignment strategies (kinematic alignment [KA] versus mechanical alignment [MA]) during total knee arthroplasty (TKA) significantly influence postoperative patellar tracking. This study aimed to compare radiological parameters of patellar tracking and clinical outcomes between KA-TKA and MA-TKA via the mini-subvastus approach.

Methods: This prospective randomized controlled study included 234 patients who underwent KA-TKA and MA-TKA from January 2022 to October 2023.

The SGLT2 inhibitor dapagliflozin suppresses endothelial cell pyroptosis mediated by the NF-κB/NLRP3 pathway through downregulation of CTSB.

Atherosclerosis (AS) is a chronic inflammatory disease, and pyroptosis-a recently discovered pro-inflammatory programmed cell death process-can exacerbate these inflammatory responses. Vascular endothelial cell pyroptosis contributes to AS progression. Cathepsin B (CTSB) is a crucial member of the cysteine protease family found in lysosomes.

A Compact-Solvation Electrolyte Under Low Concentration for High-Energy Density and Stable Potassium-Ion Batteries.

The development of potassium-ion batteries (PIBs) faces significant challenges due to the lack of suitable electrolytes to achieve satisfactory energy density and long-term stability. This work reports an innovative compact-solvation electrolyte (CSE) strategy leveraging ionic liquid-induced manipulation of solvation structures under low concentration for high-performance PIBs. The CSE, formulated with a low-salt concentration of 0.

Two-dimensional unilamellar cation-deficient metal oxide nanosheet incorporated composite polymer electrolytes for all-solid-state lithium metal batteries.

Composite polymer electrolytes (CPEs) are considered among the leading contenders for next-generation all-solid-state lithium-metal batteries. However, CPEs simultaneously face multiple significant challenges, including reduced ion transference number, insufficient ionic conductivity, and poor cycling stability, which severely limit their practical applicability. Herein, we have designed a multifunctional unilamellar inorganic nanosheets (TiO) additive for CPEs with cationic defects which is capable of simultaneously addressing all aforementioned challenges.

Coupling Water Oxidation and Oxygen Reduction for Photocatalytic HO Production over ZnInS/UiO66-NH Heterojunctions via Dual Channel Pathways.

Artificial photosynthesis of hydrogen peroxide (HO) from oxygen and water is a promising approach for converting low-density solar energy into versatile chemical energy. However, the generation of HO through a single-channel oxygen reduction reaction (ORR) is prevalent, while the water oxidation reaction (WOR) is frequently neglected. Herein, we constructed Z-scheme ZnInS/UiO66-NH (ZIS/UNH) heterojunctions, integrating the ORR and WOR dual pathways for photocatalytic HO generation without noble cocatalysts and sacrificial agents.

Surface Chemical Coordination Stabilizes Ni-Rich Cathodes for High-Energy Li-Metal Batteries.

The stability of the electrode-electrolyte interface is a critical factor influencing the electrochemical performance of Li-metal batteries. However, on the delithiated Ni-rich cathode surface, the strong catalytic effects of transition metals with coordination deficiency significantly aggravate the parasitic reactions with Li-metal-compatible ether-based electrolytes, thereby reducing the cycling stability of high-voltage Ni-rich batteries. Here, we propose an -induction mechanism to address coordination deficiency through the coupling of interfacial orbitals between molecules and the cathode surface.

LncRNA MEG3 Suppresses Prostate Cancer Progression by Mediating Macrophage Polarization via the miR-148a-3p/ARRDC3 Signaling Axis.

Long-chain non-coding RNA (LncRNA) MEG3 significantly influences tumor microenvironment (TME) dynamics and macrophage polarization. However, its specific involvement in prostate cancer (PCa) progression remains unclear. MEG3 exhibited low expression in PCa, and immune infiltration analysis revealed a positive association with M1 macrophages infiltration and a negative association with M2 macrophages infiltration.

Correlation Between Monocyte Count, Monocyte-Lymphocyte Ratio, and Other Inflammatory Cells With Sleep and Psychiatric Symptoms in First-Episode Schizophrenia Patients.

Background: More and more evidence shows that infection and immune abnormality are closely related to the increased severity of schizophrenia symptoms. This study aimed to explore the correlation between inflammatory cell counts, sleep quality, and psychiatric symptoms in first-episode schizophrenia patients.

Methods: A total of 103 first-episode schizophrenia patients (patient group) admitted to the Anhui Provincial Mental Health Center from November 2021 to August 2022 were included in the study, while 57 healthy individuals (control group) who met the criteria were recruited as the study subjects.

A Three-stage Strategy Significantly Improves Hand Movement Direction Decoding of a Single Neural Unit.

Invasive brain-computer interfaces (iBCI) can record multiple neural signals with the highest temporal and spatial resolution. However, the number of available neural units decreases with the increase in implantation time, which affects the stability of the iBCI system's control. Meanwhile, most current studies utilize a population of neural units to decode a single instruction, which limits the ability to decode multi-tasks simultaneously in complex scenarios.

Cross-Skeleton Interaction Graph Aggregation Network for Representation Learning of Mouse Social Behaviour.

Automated social behaviour analysis of mice has become an increasingly popular research area in behavioural neuroscience. Recently, pose information (i.e.

Cross-Modal Conditioned Reconstruction for Language-guided Medical Image Segmentation.

Recent developments underscore the potential of textual information in enhancing learning models for a deeper understanding of medical visual semantics. However, language-guided medical image segmentation still faces a challenging issue. Previous works employ implicit architectures to embed textual information.

Output-Based Decentralized Adaptive Event-Triggered Control of Interconnected Systems With Sensor/Actuator Failures.

This article presents a double-channel (sensor-to-controller channel and controller-to-actuator channel) event triggered control method for nonlinear interconnected systems subject to sensor and actuator faults via the backstepping technique. It should be emphasized that the utilization of triggering mechanism at the sensor side poses a challenge to the design of backstepping control, as it leads to nondifferentiable virtual control signals due to the discontinuous nature of the state/output signals received at the controller side. In contrast to existing methods, the proposed event triggering mechanism eliminates the need for computing virtual control signals at the sensor side before transmitting them to the controller side.

Electrospinning strategies targeting fibroblast for wound healing of diabetic foot ulcers.

The high incidence and prevalence of diabetic foot ulcers (DFUs) present a substantial clinical and economic burden, necessitating innovative therapeutic approaches. Fibroblasts, characterized by their intrinsic cellular plasticity and multifunctional capabilities, play key roles in the pathophysiological processes underlying DFUs. Hyperglycemic conditions lead to a cascade of biochemical alterations that culminate in the dysregulation of fibroblast phenotype and function, which is the primary cause of impaired wound healing in DFUs.