Clinical Outcome of UCBT for Children with CAEBV: A Retrospective Analysis of a Single Center.

Background: Chronic active Epstein-Barr virus (CAEBV) infection is a severe, life-threatening condition characterized by persistent Epstein-Barr virus (EBV) infection and the clonal expansion of infected T or NK cells, leading to systemic inflammation, organ damage, and complications such as hemophagocytic lymphohistiocytosis and lymphoma. Allogeneic hematopoietic stem cell transplantation (HSCT) is the only effective treatment for eradicating EBV-infected cells; however, donor availability is limited. Umbilical cord blood stem cell transplantation (UCBT) is a promising alternative owing to its rapid availability and lower complication risk.

Spectroscopic and molecular docking studies on binding interactions of camptothecin drugs with bovine serum albumin.

This study investigates the binding interactions between bovine serum albumin (BSA) and camptothecin (CPT) drugs (camptothecin, 10-hydroxycamptothecin, topotecan, and irinotecan) using UV-Vis spectroscopy, fluorescence spectroscopy, three-dimensional fluorescence spectroscopy, and molecular docking techniques. The fluorescence quenching of BSA by CPT drugs follows a static mechanism, with binding constants (K) ranging from 4.23 × 10 M (CPT) to 101.

Influence of habitat types and seasons on the composition and diversity of spontaneous species in the rural-urban fringe of Chengdu City, China.

The rural-urban fringe (RUF) is the indistinct boundary zone outside the urban area. It is also a highly vulnerable, sensitive, and ecologically valuable space, owing to the complex demographic composition, unbalanced economic development, and dramatic changes in land use and ecosystems. Different plants spontaneously emerge across RUF areas, playing an important ecological role in regulating climate and providing varied habitats for plants and animals.

Exploring the clinical outcomes and molecular characteristics of bloodstream infections: a study of sequence types, capsular types, and drug resistance in China.

Background: Bloodstream infections (BSIs) caused by have been associated with high mortality. To improve the outcomes of patients, this study explored the clinical characteristics and outcomes of patients with BSIs, as well as the phenotypic and genomic characteristics of these isolates.

Methods: A retrospective cohort study was conducted involving BSIs cases from 2020 to 2023 in a tertiary hospital.

Gut microbiota-derived imidazole propionate: an emerging target for the prevention and treatment of cardiometabolic diseases.

Despite significant advancements in prevention and treatment, cardiometabolic diseases continue to pose a high burden of incidence and mortality. The chronic progression of these diseases necessitates the identification of early and complementary therapeutic targets to elucidate and mitigate residual risks in patient care. The gut microbiota acts as a sentinel between internal and external environments, transmitting modified risks associated with these factors to the host.

Evaluating the effectiveness of integrated traditional Chinese and Western treatment based on symptom grading: a study protocol for a multi-center, randomized controlled trial of patients with depressive disorder.

Background: Approximately one-third of depressed individuals receive treatment globally. The application rate of traditional Chinese medicine (TCM) for treating depression globally remains relatively low. The proposed study presents a pilot trial to evaluate the effectiveness of interventions at different levels in improving depression status in community populations.

Risk Factors and Predictive Analysis of Acute Severe Abdominal Pain After Hepatic Artery Infusion Chemotherapy in Patients with Hepatocellular Carcinoma.

Purpose: To investigate the incidence patterns and risk factors of acute abdominal pain following hepatic arterial infusion chemotherapy (HAIC) in liver cancer patients and develop a preliminary prediction model for post-HAIC abdominal pain.

Patients And Methods: Four hundred hepatocellular carcinoma patients who underwent HAIC at the Affiliated Cancer Hospital of Shandong First Medical University from January 2021 to March 2023 were retrospectively analyzed. The patients were categorized into two groups (abdominal pain and no abdominal pain) based on the occurrence of acute moderate to severe abdominal pain within 24 h after HAIC.

Divergent roles of PKM2 in regulating PD-L1 and PD-L2 expression and their implications in human and mouse cancer models.

Cancer cells evade immune detection through checkpoint molecules like PD-L1 and PD-L2 which suppress T-cell activation. While PD-L1 is well-studied, the role of PD-L2 remains unclear. Pyruvate kinase M2 (PKM2), a metabolic enzyme, influences immune checkpoint regulation, but its role in PD-L1 and PD-L2 modulation is not well defined.

Poly-guanine RNAs form ultra-stable intermolecular G-quadruplexes to suppress RNA function at low environmental temperature.

Intramolecular G-quadruplexes (G4) are four-stranded nucleic acid structures formed by guanine-rich sequences that play crucial roles in various biological processes, such as gene regulation and genome stability. However, the formation and function of intermolecular G4, especially in RNA, remain less understood. In this study, it was demonstrated that poly-guanine (poly-G) RNA sequences can form ultra-stable intermolecular G4 structures, particularly when the guanine content exceeds five.

Modular Assembly of Chalcones, Tosylhydrazones, and KSCN/KSeCN for the Synthesis of Trisubstituted Imidazo[2,1-][1,3,4]thiadiazoles/selenadiazoles.

A modular protocol that achieved the efficient synthesis of imidazo[2,1-][1,3,4]thiadiazoles/selenadiazoles via using easily available -tosylhydrazones, chalcone derivatives, and KSCN/KSeCN is proposed. The method overcomes the elongated synthesis steps and prefunctionalized synthons of previous methods. It solves the problem of traditional preparation methods, which makes it difficult to synthesize thickened selenium-containing heterocyclic molecules, further expanding the number of members in its family.

Hierarchical DNA Octahedral Nanoplatform for in Situ Biosensing and Clinical Monitoring of Acute Lymphoblastic Leukemia.

Developing nanoscale platforms with high integration, assembly efficiency, and structural stability for performing complex computations in specific cells remains a significant challenge. To address this, the Three-dimensional Hierarchical Octahedral Robotic (THOR) DNA nanoplatform is introduced, which integrates targeting, logic computation, and sensing modules within a single framework. This nanoplatform specifically binds to cancer cell surface proteins, releasing aptamer-linked fuel chains to initiate subsequent computational processes.

Rare mixed dementia: A case report.

Background: Autoimmune encephalitis (AE) is a rare and recently described neuroinflammatory disease associated with specific autoantibodies. Anti-leucine-rich glioma inactivated 1 (anti-LGI1) encephalitis is a rare but treatable type of AE discovered in recent years. Alzheimer's disease (AD) is a degenerative brain disease and the most common cause of dementia.

Patients With Chronic Lateral Ankle Instability and Small Osteochondral Lesions of the Talus Obtain Good Postoperative Results: A Minimum 10-Year Follow-up With Radiographic Evidence.

Background: Few studies reported the long-term clinical outcomes and joint degeneration of patients with chronic lateral ankle instability (CLAI) and small osteochondral lesions of the talus (OLTs) following simultaneous open modified Broström-Gould (MBG) surgery and arthroscopic bone marrow stimulation (BMS). The purpose of this study was to study the long-term results of patients after BMS and BMG surgery, and to further evaluate the potential effect of OLT size on postoperative results.

Methods: In this retrospective study, 110 CLAI patients were divided into 57 patients with OLTs (including 24 patients having combined small osteochondral lesions of the tibial plafond) receiving simultaneous BMS and MBG surgeries (BMS+MBG group), and 53 patients without OLTs receiving isolated open MBG surgery (MBG group).

[Clinical sub-phenotypes of acute kidney injury in children and their association with prognosis].

Objectives: To investigate the clinical sub-phenotype (SP) of pediatric acute kidney injury (AKI) and their association with clinical outcomes.

Methods: General status and initial values of laboratory markers within 24 hours after admission to the pediatric intensive care unit (PICU) were recorded for children with AKI in the derivation cohort (=650) and the validation cohort (=177). In the derivation cohort, a least absolute shrinkage and selection operator (LASSO) regression analysis was used to identify death-related indicators, and a two-step cluster analysis was employed to obtain the clinical SP of AKI.

Electron-phonon coupling and thermal transport properties of GaN/AlGaN heterojunction under strain regulation.

In the study of GaN/AlGaN heterostructure thermal transport, the interference of strain on carriers cannot be ignored. Although existing research has mainly focused on the intrinsic electronic and phonon behavior of the materials, there is a lack of studies on the transport characteristics of the electron-phonon coupling in heterostructures under strain control. This research comprehensively applies first-principles calculations and the Boltzmann transport equation simulation method to deeply analyze the thermal transport mechanism of the GaN/AlGaN heterojunction considering in-plane strain, with particular attention to the regulatory role of electron-phonon coupling on thermal transport.

The nuclear matrix stabilizes primed-specific genes in human pluripotent stem cells.

The nuclear matrix, a proteinaceous gel composed of proteins and RNA, is an important nuclear structure that supports chromatin architecture, but its role in human pluripotent stem cells (hPSCs) has not been described. Here we show that by disrupting heterogeneous nuclear ribonucleoprotein U (HNRNPU) or the nuclear matrix protein, Matrin-3, primed hPSCs adopted features of the naive pluripotent state, including morphology and upregulation of naive-specific marker genes. We demonstrate that HNRNPU depletion leads to increased chromatin accessibility, reduced DNA contacts and increased nuclear size.

Preoperative Computed Tomography Radiomics-Based Models for Predicting Microvascular Invasion of Intrahepatic Mass-Forming Cholangiocarcinoma.

Objectives: The aim of the study is to investigate the ability of preoperative CT (Computed Tomography)-based radiomics signature to predict microvascular invasion (MVI) of intrahepatic mass-forming cholangiocarcinoma (IMCC) and develop radiomics-based prediction models.

Materials And Methods: Preoperative clinical data, basic CT features, and radiomics features of 121 IMCC patients (44 with MVI and 77 without MVI) were retrospectively reviewed. The loading and display of CT images, delineation of the volume of interest, and feature extraction were performed using 3D Slicer.

Optical biosensor arrays based on nanozymes for environmental monitoring and food safety detection: principles, design, and applications.

Typical biosensing platforms are based on the "lock-and-key" approach, providing high specificity and sensitivity for environmental and food safety monitoring. However, they are limited in their ability to detect multiple analytes simultaneously. With the use of pattern identification methods, biosensor arrays can detect faint fluctuations caused by multiple analytes with similar properties in complex systems.

Acupuncture for hormonal readiness and gut microbiota in obese polycystic ovary syndrome: an open-label, randomized controlled trial.

Objective: To explore whether acupuncture combined with clomiphene can reduce the luteinizing hormone-to follicle-stimulating hormone ratio and impact the gut microbiota in patients with obese polycystic ovary syndrome.

Methods: This open-label, randomized, parallel-group controlled trial included 86 women aged 20-40 years with obese polycystic ovary syndrome and 19 healthy controls. Participants were randomly assigned to either an acupuncture combined with clomiphene group or a clomiphene-only group, with a healthy control group for comparison.

Cholesterol mediates the potential adverse influence of graphene quantum dots on placental lipid membrane model.

Nanomaterial-biomembrane interactions constitute a critical biological process in assessing the toxicity of such materials in theoretical studies. However, many investigations simplify these interactions by using membrane models containing only one or a few lipid types, deviating significantly from the complexity of real membrane compositions. In particular, cholesterol, a ubiquitous lipid essential for regulating membrane fluidity and closely linked to various diseases, is often overlooked.

Influencing factors of elderly hospitalized psychiatric patients complicated with physical disease: a cross-sectional study of 332 patients in Guangzhou China.

Objective: To investigate the influencing factors of the number of physical diseases in elderly hospitalized psychiatric patients.

Methods: A total of 332 hospitalized elderly patients with mental illness were enrolled in this study. The number of combined physical diseases was divided into three groups, which were 0, 1 ~ 2, and ≥ 3.