PRAF2 as a novel biomarker for breast cancer with machine learning and experimentation validation.

Background: Breast cancer (BC) is the most prevalent malignancy in women. Potential therapeutic targets for BC are of great significance. In our previous study, we found that prenylated rab acceptor 1 domain family member 2 (PRAF2) is an oncogene in BC.

RNA-seq and Ribosome Profiling Reveal the Translational Landscape of Rice in Response to Rice Stripe Virus Infection.

Rice is a crucial staple food for over half the global population, and viral infections pose significant threats to rice yields. This study focuses on the Rice Stripe Virus (RSV), which is known to drastically reduce rice productivity. We employed RNA-seq and ribosome profiling to analyze the transcriptional and translational responses of RSV-infected rice seedlings.

Identification and Genome Sequencing of Novel Virulent Strains of pv. Causing Rice Bacterial Blight in Zhejiang, China.

pv. () is the causative agent of rice bacterial blight (RBB), resulting in substantial harvest losses and posing a challenge to maintaining a stable global supply. In this study, strains isolated from Shaoxing, Quzhou, and Taizhou, where RBB occurred most frequently in Zhejiang Province in 2019, were selected as the subjects of research.

Spatial transcriptomics in breast cancer: providing insight into tumor heterogeneity and promoting individualized therapy.

A comprehensive understanding of tumor heterogeneity, tumor microenvironment and the mechanisms of drug resistance is fundamental to advancing breast cancer research. While single-cell RNA sequencing has resolved the issue of "temporal dynamic expression" of genes at the single-cell level, the lack of spatial information still prevents us from gaining a comprehensive understanding of breast cancer. The introduction and application of spatial transcriptomics addresses this limitation.

N6-methyladenosine (m6A) modification: Emerging regulators in plant-virus interactions.

N6-methyladenosine (m6A), a reversible epigenetic modification, is widely present on both cellular and viral RNAs. This modification undergoes catalysis by methyltransferases (writers), removal by demethylases (erasers), and recognition by m6A-binding proteins (readers), ultimately influencing the fate and function of modified RNA molecules. With recent advances in sequencing technologies, the genome-wide mapping of m6A has become possible, enabling a deeper exploration of its roles during viral infections.

Preparation of Patchouli Oil Microemulsion Gel and Its Topical Application to Ameliorate Atopic Dermatitis in Mice.

Patchouli oil (PO) is a natural substance famous for its immune-enhancing and anti-inflammatory effects. Atopic dermatitis (AD) is characterized by epidermal gene mutations, skin barrier dysfunction, and immune dysregulation, making patchouli volatile oil a potential candidate for AD treatment. Initially, PO was mixed with ethyl oleate (EO), castor oil ethoxylated ether-40 (EL-40), anhydrous ethanol, and water to form a patchouli oil microemulsion (PO-ME) system.

Protective Effects of Polysaccharides From (Turner) C. Agardh Against Alcohol-Induced LO2 Cell Damage.

The study aimed to explore the protective impact of polysaccharide derived from (Turner) C. Agardh (SHP) against ethanol-induced injury in LO2 hepatocytes, along with its potential mechanism of action. A model of alcoholic injury in LO2 cells was established to assess the shielding effect of SHP against liver injury induced by alcohol.

Selenium nanoparticles decorated by fucoidan induce ferroptosis in HepG2 cells.

Unlike apoptosis, necrosis and autophagy, ferroptosis is a novel type of regulated cell death, and the mechanism by which selenium nanoparticles induce ferroptosis in cancer cells has rarely been investigated. To investigate the mechanism of inhibition of HepG2 cell proliferation by fucoidan-selenium nanoparticles (FD-SeNPs) by inducing ferroptosis. The mechanism was explored by detecting ROS, MDA, GSH and Fe and utilizing TEM and Western blot assay.

Host Metabolic Alterations Mediate Phyllosphere Microbes Defense upon pv Infection.

Rice bacterial leaf blight, caused by pv (), is a significant threat to global food security. Although the microbiome plays an important role in protecting plant health, how the phyllosphere microbiome is recruited and the underlying disease resistance mechanism remain unclear. This study investigates how rice phyllosphere microbiomes respond to pathogen invasion through a comprehensive multiomics approach, exploring the mechanisms of microbial defense and host resistance.

The plant signal peptide CLE7 induces plant defense response against viral infection in Nicotiana benthamiana.

In plants, small peptides are important players in the plant stress response, yet their function in plant antiviral responses remains poorly understood. Here, we identify that the plant small peptide, CLAVATA3/ESR-RELATED 7 (CLE7), enhances plant resistance to Chinese wheat mosaic virus infection in Nicotiana (N.) benthamiana.

Ononin Inhibits Tumor Bone Metastasis and Osteoclastogenesis By Targeting Mitogen-Activated Protein Kinase Pathway in Breast Cancer.

Breast cancer (BC) often spreads to bones, leading to bone metastasis (BM). Current targeted therapies have limited effectiveness in the treatment of this condition. Osteoclasts, which contribute to bone destruction, are crucial in supporting tumor cell growth in the bones.

Efficacy and safety of methotrexate in the treatment of proliferative vitreoretinopathy: a systematic review.

Proliferative Vitreoretinopathy (PVR) is a major complication of surgical repair for Rhegmatogenous Retinal Detachment (RRD). Methotrexate (MTX), a folate antimetabolite, has shown promise in targeting the pathological processes involved in PVR, such as cell proliferation inhibition, fibrosis and anti-inflammation. Systematic review examines the use of MTX in PVR by analysing different administration methods and outcomes.

Thermal tuning behavior and robust control of a single-soliton microcomb with a modulated sideband.

A chip-scaled single-soliton microcomb source promises wide applications in various fields. We demonstrate the deterministic single-soliton generation from both pump forward and backward tunings via sideband thermal compensation. The total soliton existing range (SER) is effectively expanded due to the thermal-lock effect and remains nearly the same regardless of the soliton states.

A viral protein activates the MAPK pathway to promote viral infection by downregulating callose deposition in plants.

Mitogen-activated protein kinase (MAPK) cascades are evolutionarily conserved in both plants and animals and play critical roles in activating innate immunity to defend against various pathogens. However, the role of MAPK cascades in positively regulating or enhancing viral infections in plants is unclear. In this study, we investigate the involvement of MAPK cascades in infection by the positive-strand RNA virus tomato chlorosis virus (ToCV).

A Stinkbug Salivary Protein Is Indispensable for Insect Feeding and Activates Plant Immunity.

Salivary proteins secreted by phytophagous insects play pivotal roles in plant-insect interactions. A salivary protein RpSP27, from the stinkbug Riptortus pedestris, a devastating pest on soybean, was selected for studying due to its ability to induce cell death and activate immune responses in plants. RpSP27 localized to the endoplasmic reticulum and triggered reactive oxygen species burst.

Diversity and transmissibility of RNA viruses in the small brown planthopper, .

Unlabelled: While a considerable number of viruses have been recently discovered in hematophagous insects, there remains insufficient research on virus diversity and their association with phytophagous insect hosts. In this study, we conducted a systematic investigation of the RNA virome in the small brown planthopper (SBPH), , an important vector of plant viruses. We successfully identified a total of 22 RNA viruses, including 17 novel viruses, from various families.

Accuracy and safety of implant placement with a novel semi-autonomous robotic-assisted surgical system: A translational research study.

Statement Of Problem: Translational studies evaluating the feasibility, accuracy, and safety of semi-autonomous implant robots, from model test to animal experiment and clinical trial, are currently lacking.

Purpose: The purpose of this study was to evaluate the accuracy and safety of a novel semi-autonomous robotic-assisted surgical system (sa-RASS) in implant placement.

Material And Methods: A translational study was conducted to assess the application of the sa-RASS in a model test, an animal experiment, and a clinical controlled trial.

Fiber-optic radio frequency transfer with enhanced frequency stability using fiber Brillouin amplifiers.

The frequency stability of long-distance two-way fiber-optic radio frequency (RF) transfer is directly affected by the optical signal-to-noise ratio (OSNR) of optical amplifiers. In this paper, we have proposed a stimulated Brillouin scattering (SBS)-based optical amplification scheme with high OSNR for two-way fiber-optic RF frequency transfer over single mode fibers (SMF). At the remote and local site, the modulated carrier transferred from the opposite was amplified and then frequency upshifted by Brillouin frequency shift (BFS) for pump generation.

Topical application of 666-15, a potent inhibitor of CREB, alleviates alkali-induced corneal neovascularization.

Corneal neovascularization (CNV) is a dynamically regulated process that arises due to a disruption in the equilibrium between pro-angiogenic and anti-angiogenic factors. Various cytokines are released by vascular endothelial cells and macrophages in damaged cornea, ultimately inducing CNV. The cAMP-response element-binding protein (CREB), a nuclear transcription factor, potentially impacts tumor angiogenesis by modulating the secretion of angiogenic proteins.

Accuracy of a Novel Semi-Autonomous Robotic-Assisted Surgery System for Single Implant Placement: a Case Series.

Objective: This study aimed to evaluate the accuracy of dental implant placement at single-tooth sites using a novel semi-autonomous robotic-assisted surgery system (sa-RASS).

Methods: Patients with single missing teeth were included. Cone-beam computed tomography (CBCT) was performed prior to surgery using a U-shaped silicone tube to develop a virtual implant placement and drilling plan.

Development of a pH-Sensitive Nanoparticle via Self-Assembly of Fucoidan and Protamine for the Oral Delivery of Insulin.

: Oral insulin delivery has received much attention over the past 20 years due to its high compliance. The aim of this study is to prepare nanoparticles for the oral delivery of insulin; : Fucoidan and protamine were used to prepare a pH-sensitive nanoparticle via self-assembly. The secondary structure and in vitro stability of the nanoparticles were characterized using FTIR, XRD, ITC, and TEM.