Endoscopic surgery affects the gut microbiota and its metabolism in breast cancer patients.

Background: Despite the advantages of endoscopic surgery in reducing trauma and enhancing recovery for breast cancer patients, its impact on gut microbiota, which is crucial for health and estrogen metabolism, remains unclear. Further investigation is necessary to fully understand this impact and its implications.

Materials And Methods: Between June and December 2022, fecal samples were collected from 20 patients who underwent endoscopic surgery.

Comparative analysis of petal phytoconstituents reveals insights into the characteristics of an under-reported edible old rose variety native to Chongqing, China.

Chongqing Old Rose is an ancient edible rose variety native to Chongqing, China, but is under-reported. Further evidence is required to fully establish its potential benefits. The complete metabolic profiles were examined for comparative analysis between the Old Rose and three rose cultivars.

The effect of microenvironmental viscosity on the emergence of colon cancer cell resistance to doxorubicin.

The colon possesses a unique physiological environment among human organs, where there is a highly viscous body fluid layer called the mucus layer above colonic epithelial cells. Dysfunction of the mucus layer not only contributes to the occurrence of colorectal cancer (CRC) but also plays an important role in the development of chemoresistance in CRC. Although viscosity is an essential property of the mucus layer, it remains elusive how viscosity affects chemoresistance in colon cancer cells.

Regulation of antigen presentation and interleukin 10 production in murine dendritic cells via the oxidative stimulation of cell membrane using a polycation-porphyrin-conjugate-immobilized cell culture dish.

Tolerogenic dendritic cells with professional antigen presentation via major histocompatibility complex molecules, co-stimulatory molecules (CD80/86), and interleukin 10 production have attracted significant attention as cellular therapies for autoimmune, allergic, and graft-versus-host diseases. In this study, we developed a cell culture dish equipped with polycation-porphyrin-conjugate-immobilized glass (PA-HP-G) to stimulate immature murine dendritic cell (iDCs). Upon irradiation with a red light at 635 nm toward the PA-HP-G surface, singlet oxygen was generated by the immobilized porphyrins on the PA-HP-G surface.

Parental acceptance of school-based universal depression screening for children and adolescents in primary and secondary school in China.

Introduction: School-based universal depression screening (SBUDS) is an effective method for early identification of depression. As parents are the primary decision-makers for their children's acceptance of healthcare services, this study aims to examine rural and urban parental acceptance of SBUDS.

Methods: The study assessed parental acceptance of SBUDS for their children and its association with self-reported parental perception of depression (i.

SlUPA-like, a bHLH Transcription Factor in Tomato (), Serves as the Crosstalk of GA, JA and BR.

The bHLH (basic Helix-Loop-Helix) transcription factor serves as pivotal controller in plant growth and development. In a previous study, the overexpression of in L. Ailsa Craig (AC) altered the JA (Jasmonic acid) response and endogenous GA (Gibberellic acid) content.

Mycorrhizal dominance influences tree species richness and richness-biomass relationship in China's forests.

Mycorrhizal associations drive plant community diversity and ecosystem functions. Arbuscular mycorrhiza (AM) and ectomycorrhiza (EcM) are two widespread mycorrhizal types and are thought to differentially affect plant diversity and productivity by nutrient acquisition and plant-soil feedback. However, it remains unclear how the mixture of two mycorrhizal types influences tree diversity, forest biomass, and their relationship at large spatial scales.

Novel Insights into Pigment Composition and Molecular Mechanisms Governing Flower Coloration in Rose Cultivars Exhibiting Diverse Petal Hues.

The pigmentation of various components leads to different colors of roses. However, the intricate molecular machinery and metabolic pathways underlying rose pigmentation remain largely unexplored. In this study, we determined that pink and black-red petals contain abundant anthocyanins, reaching concentrations of 800 μg/g and 1400 μg/g, respectively, significantly surpassing those in white and yellow petals.

Effective disruption of cancer cell membranes by photodynamic therapy with cell membrane-adhesive photosensitizer.

Photodynamic therapy (PDT) is a noninvasive cancer treatment modality that involves the administration of photosensitizers and light irradiation. Previously, we established a polycation-containing hematoporphyrin (aHP) formulation that demonstrated superior antitumor efficacy , than the original hematoporphyrin (HP). In this study, we investigated underlining mechanisms of the high antitumor effect of aHP using cell experiments.

Silencing of Reduces the Tolerance to Drought and Salt Stress via the ABA Pathway in Tomato.

The MYB transcription factor family plays a crucial regulatory role in plant growth, development, biological progress, and stress responses. Here, we identified a R2R3-MYB transcription factor gene, , from tomato and characterized its function by gene silencing via RNA interference (RNAi). The results exhibited that the silencing of reduced the sensitivity of tomato seedlings to exogenous ABA.

Scaffolds functionalized with matrix metalloproteinase-responsive release of miRNA for synergistic magnetic hyperthermia and sensitizing chemotherapy of drug-tolerant breast cancer.

Combining hyperthermia and chemotherapy for maximum anticancer efficacy remains a challenge because drug-tolerant cancer cells often evade this synergistic treatment due to drug resistance and asynchronous drug release. In this study, multifunctional scaffolds were designed to efficiently treat drug-tolerant breast cancer by improving the sensitization of breast cancer cells and synchronizing anticancer drug release with magnetic hyperthermia. The scaffolds contained microRNA-encapsulated matrix metalloproteinase-cleavable liposomes, doxorubicin-encapsulated thermoresponsive liposomes and FeO nanoparticles.

CT imaging using variable helical pitch scanning for lower extremity arterial disease: Reduced contrast medium dose, improved image quality and diagnostic accuracy.

Objectives: This study aimed to explore the feasibility of reducing contrast medium (CM) volume, improving image quality and diagnostic accuracy using variable helical pitch (VHP) scanning for patients with lower extremity arterial disease (LEAD).

Materials And Methods: Eighty patients who underwent lower extremity CT angiography (CTA) were prospectively enrolled and randomly assigned to either the VHP group (n = 40) or the conventional group (n = 40). Quantitative parameters and qualitative scores were compared between the two groups.

Knockdown of Accelerates Dark-Induced Tomato Leaf Senescence by Affecting the ABA Pathway.

N6-methyladenosine (mA) is a widespread post-transcriptional modification in eukaryotic mRNAs. Proteins with the YTH structural domain act as mA-binding proteins by recognizing the mA modification and regulating mRNA through this recognition. In this study, , a prototypical mA -binding protein gene in the YTH family was expressed in various tissues, and subcellular localization analyses indicated that the SlYTHDF2 protein was localized in the nucleus and cytoplasm.

Influence of viscosity on adipogenic and osteogenic differentiation of mesenchymal stem cells during 2D culture.

Accumulatively, cellular behaviours triggered by biochemical cues have been widely explored and the focus of research is gradually shifting to biophysical cues. Compared to physical parameters such as stiffness, substrate morphology and viscoelasticity, the influence of viscosity on cellular behaviours is relatively unexplored and overlooked. Thus, in this study, the influence of viscosity on the adipogenic and osteogenic differentiation of human mesenchymal stem cells (hMSCs) was investigated by adjusting the viscosity of the culture medium.

Association between functional dependence and cardiovascular disease among middle-aged and older adults: Findings from the China health and retirement longitudinal study.

Background: The effect of different functional dependency types on cardiovascular disease (CVD) is largely unknown. Here, we aimed to investigate the association between functional dependence and CVD among middle-aged and older adults by conducting a cross-sectional and longitudinal study.

Methods: The study sample comprised 16,459 individuals of ≥40 years (including 10,438 without CVD) who had participated in the 2011 China Health and Retirement Longitudinal Study (CHARLS).

Pentagon-Rich Caged Carbon Catalyst for the Oxygen Reduction Reaction in Acidic Electrolytes.

The interaction between electron spin and oxygen molecules in non-platinum catalysts, particularly carbon catalysts, significantly influences the catalytic performance of the oxygen reduction reaction (ORR). A promising approach to developing high-performance catalysts involves introducing five-membered ring structures with spin into graphitic carbons. In this study, we present the successful synthesis of cage-like cubic carbon catalysts enriched with pentagon structures using pentagon ring-containing C and a NaCl template.

Detection of serum SNHG22 and its correlation with prognosis of non-small cell lung cancer.

Background: Lung cancer accounts for a significant proportion of cancer-related deaths in China, with the majority of the cases being classified as non-small cell lung cancer (NSCLC). The study aimed to investigate the expression of serum SNHG22 in patients with NSCLC, and its molecular mechanism and prognostic potential in NSCLC.

Methods: Admitted 125 NSCLC patients were selected for the study, along with 125 healthy individuals in the same period.

Overexpression of Increases Drought and Salt Tolerance and Affects Fruit Dehiscence in Tomatoes.

The bHLH transcription factors are important plant regulators against abiotic stress and involved in plant growth and development. In this study, , a gene coding for a prototypical DNA-binding protein in the bHLH family, was isolated, and -overexpression tomato (-OE) plants were generated by Agrobacterium-mediated genetic transformation. transgenic lines manifested higher osmotic stress tolerance than the wild-type plants, estimated by higher relative water content and lower water loss rate, higher chlorophyll, reducing sugar, starch, proline, soluble protein contents, antioxidant enzyme activities, and lower MDA and reactive oxygen species contents in the leaves.

SlFSR positively regulates ethylene biosynthesis and lycopene accumulation during fruit ripening in tomato.

Transcription factors (TFs) are crucial for regulating fruit ripening in tomato (Solanum lycopersicum). The GRAS (GAI, RGA, and SCR) TFs are involved in various physiological processes, but their role in fruit ripening has seldom been reported. We have previously identified a gene encoding GRAS protein named SlFSR (Fruit Shelf-life Regulator), which is implicated in fruit ripening by regulating cell wall metabolism; however, the underlying mechanism remains unclear.

The knockout of SlMTC impacts tomato seed size and reduces resistance to salt stress in tomato.

Members of the MT-A70 family are key catalytic proteins involved in mA methylation modifications in plants. They play diverse roles at the posttranscriptional level by regulating RNA secondary structure, selective splicing, stability, and translational efficiency, which collectively affect plant growth, development, and stress responses. In this study, we explored the function of the gene SlMTC, a Class C member of the MT-A70 family, in tomatoes by using CRISPR/Cas9 technology.

Efficacy and safety analysis of AKT inhibitor in triple-negative breast cancer: A meta-analysis and systematic review.

Objective: To determine the clinical benefit of monotherapy with AKT inhibitors in patients diagnosed with triple-negative breast cancer (TNBC).

Methods: A systematic search was conducted in PubMed, Embase, and Cochrane Library for articles reporting treatment with AKT inhibitors in TNBC. The primary endpoint was progression-free survival and overall survival (OS).

A New Case Report of a CLCNKB Complex Heterozygous Mutation in Adult-Onset Type III Bartter Syndrome.

Background: Type III Bartter syndrome (BS) is an autosomal recessive renal tubular disease caused by the mutation of the chloride voltage-gated channel Kb (CLCNKB) gene. This condition is characterized by renal sodium loss, hypokalemia, metabolic alkaliosis, high renin, and high aldosterone levels.

Methods: We report a case of adult type III BS caused by a novel complex heterozygous mutation of the CLCNKB gene.