Tissue-Specific Profiling of -GlcNAcylated Proteins in Using TurboID-OGA.

Protein GlcNAcylation is a prevalent and dynamic post-translational modification that targets a multitude of nuclear and cytoplasmic proteins. Through the modification of diverse substrates, GlcNAcylation plays a pivotal role in essential cellular processes, including transcription, translation, and protein homeostasis. Dysregulation of GlcNAc homeostasis has been implicated in a variety of diseases, including cardiovascular diseases, cancer, and neurodegenerative diseases.

Chronic unpredictable mild stress induces anxiety-like behavior in female C57BL/6N mice, accompanied by alterations in inflammation and the kynurenine pathway of tryptophan metabolism.

Chronic stress can impact brain function through various mechanisms, contributing to the development of anxiety disorders. Chronic unpredictable mild stress (CUMS) is a well-established model for studying the effects of chronic stress. This study assessed the impacts of different durations of CUMS on anxiety-like behavior, inflammation, and tryptophan metabolism in female C57BL/6N mice.

Rapeseed and Palm Oils Can Improve the Growth, Muscle Texture, Fatty Acids and Volatiles of Marine Teleost Golden Pompano Fed Low Fish Oil Diets.

This study evaluated the effects of different lipid sources-fish oil (FO), soybean oil, rapeseed oil, and palm oil-on the growth and muscle quality of golden pompano () cultured in offshore cages for 10 weeks. Three diets (D1-D3) were formulated: D1 used only fish oil, D2 blended fish, rapeseed oil, and palm oil, and D3 combined fish and soybean oils. Fish in the D1 group showed the highest weight gain, specific growth rate, and muscle protein content, significantly outperforming D3.

Effects of Aqua-Glycerol Uptake Facilitator Protein GlpF on Spore Germination of .

Killing spores is an important challenge for the development of the food industry. After germination, the resistance of spores disappears and they are more easily killed, which is currently the main strategy for their destruction. Therefore, study of the mechanism of spore germination is of great significance for improving methods of spore inactivation.

Advancements in the application of artificial intelligence in the field of colorectal cancer.

Colorectal cancer (CRC) is a prevalent malignant tumor in the digestive system. As reported in the 2020 global cancer statistics, CRC accounted for more than 1.9 million new cases and 935,000 deaths, making it the third most common cancer worldwide in terms of incidence and the second leading cause of cancer-related deaths globally.

Optimizing Triple-Negative Breast Cancer Therapy via Ultrasound-Enhanced Piezocatalysis for Targeted Chemodrug Release.

Introduction: Triple-negative breast cancer (TNBC) is known for its high malignancy, limited clinical treatment options, and poor chemotherapy outcomes. Although some advancements have been made using nanotechnology-based chemotherapy for TNBC treatment, the controlled and on-demand release of chemotherapeutic drugs at the tumor site remains a challenge.

Methods: We manufactured DOX/BaTiO@cRGD-Lip (DBRL) nanoparticles as an ultrasound (US)-controlled release platform targeting the delivery of Doxorubicin (DOX) for TNBC treatment.

The molecular mechanism of ginsenoside Rh2 and its octyl ester derivative on anti-angiogenesis in cancer treatment: The battle between PI3K and ROS.

Background: The cancer treatments that target tumor associated angiogenesis (TAA) induced by vascular endothelial growth factor A (VEGFA) have become valued. Ginsenoside Rh2 has been proved to inhibit TAA through VEGFA. However, the underlying mechanisms remain unclear.

Green Electrode Processing Enabled by Fluoro-Free Multifunctional Binders for Lithium-Ion Batteries.

The eco-friendly processing of conjugated polymer binder for lithium-ion batteries demands improved polymer solubility by introducing functional moieties, while this strategy will concurrently sacrifice polymer conductivity. Employing the polyfluorene-based binder poly(2,7-9,9 (di(oxy-2,5,8-trioxadecane))fluorene) (PFO), soluble in water-ethanol mixtures, a novel approach is presented to solve this trade-off, which features integration of aqueous solution processing with subsequent controlled thermal-induced cleavage of solubilizing side chains, to produce hierarchically ordered structures (HOS). The thermal processing can enhance the intermolecular π-π stacking of polyfluorene backbone for better electrochemical performance.

lncRNA ACVR2B-AS1 modulates thyroid cancer progression by regulating miR-195-5p.

Background: lncRNAs are key regulators in thyroid cancer (TC). While lncRNA ACVR2B-AS1 has been proposed as a potential TC biomarker, its role remains underexplored. This study aims to clarify its clinical significance in TC and investigate its molecular mechanism.

A miniaturized MEMS accelerometer with anti-spring mechanism for enhancing sensitivity.

Anti-spring mechanisms are widely used for improving the noise performance of MEMS accelerometers due to their stiffness softening effect. However, the existing mechanisms typically require large bias force and displacement for achieving stiffness softening, leading to large device dimensions. Here, we propose a novel anti-spring mechanism composed of two pre-shaped curved beams connected in a parallel configuration, which can achieve stiffness softening without requiring large bias force and displacement.

Fibronectin Mediates Endothelial-to-Mesenchymal Transition in Retina Angiogenesis.

Purpose: The purpose of this study was to investigate the role of endothelial-mesenchymal transition (EndoMT) in pathological retinal angiogenesis and identify key molecular mediators in retina angiogenesis.

Methods: RNA sequencing (RNA-seq) was performed on retinal tissue from an oxygen-induced retinopathy (OIR) mouse model to analyze gene expression patterns. The Gene Set Enrichment Analysis was used to examine the correlation between epithelial-mesenchymal transition (EMT) and angiogenesis gene sets.

Modeling the cost of inaction in treating obesity in Canada.

Background: Obesity prevalence continues to rise in Canada, highlighting a growing public health concern. This study updates estimates of the societal cost of inaction in treating obesity, emphasizing the significant economic burden stemming from both direct healthcare costs and indirect productivity losses.

Methods: We combined data from national surveys and published literature to estimate the 2023 national economic implications of obesity.

Maternal consumption of glycerol monolaurate optimizes milk fatty acid profile and enhances piglet gut health in association with G protein-coupled receptor 84 (GPR84) activation.

This study evaluated the effect of maternal glycerol monolaurate (GML) supplementation during late gestation and lactation on sow reproductive performance, transfer of immunity and redox status, milk fat and fatty acid profile, and fecal microbiota. Eighty multiparous sows (Landrace × Large white) were randomly allocated to two treatment groups (with or without 1000 mg/kg GML) with 40 replicates per treatment. The feeding experiment lasted from d 85 of gestation (G85) to d 23 of lactation (L23).

Cross-Skeleton Interaction Graph Aggregation Network for Representation Learning of Mouse Social Behaviour.

Automated social behaviour analysis of mice has become an increasingly popular research area in behavioural neuroscience. Recently, pose information (i.e.

Thinking Like Sonographers: Human-centered CNN models for gout diagnosis from musculoskeletal ultrasound.

We explore the potential of deep convolutional neural network (CNN) models for differential diagnosis of gout from musculoskeletal ultrasound (MSKUS). Our exhaustive study of state-of-the-art (SOTA) CNN image classification models for this problem reveals that they often fail to learn the gouty MSKUS features, including the double contour sign, tophus, and snowstorm, which are essential for sonographers' decisions. In this study, we establish a human-centered adjusting framework to make CNN models diagnosis gout in the way of thinking like sonographers.

Outperforming Traditional Staging: A Novel Nomogram for HR-Positive Breast Cancer.

Background: Hormone receptor-positive breast cancer (HR-positive BC), the most prevalent subtype, typically has a favorable prognosis. However, treatment decision-making and survival prediction remain challenging due to the limitations of traditional staging systems like AJCC. Improved prognostic tools are needed to enhance individualized risk stratification.

Sex differences in reward-based operant conditioning performance and neurotransmitter changes following chronic sleep restriction stress in rats.

Background: Sleep deprivation significantly impairs cognitive function, which disrupts daily life. However, sex differences in these impairments are not well understood, as most preclinical studies primarily use male animals, neglecting potential differences between sexes. This study aims to investigate sex-specific differences in cognitive function under sleep deprivation using reward-based operant conditioning tasks.

[The Significance of miR-122 in Early Screening of Hepatitis B Infection in Blood Donors].

Objective: To study the correlation between miR-122 and early HBV infection and analyze its application value in early infection of voluntary blood donors.

Methods: A total of 150 samples from voluntary blood donors in Fujian Blood Center from May 2021 to July 2022 were collected and divided into group N (normal group), group E (ELISA single positive group), and group D (both ELISA and nucleic acid positive group), and the general information of the three groups of blood donors was collected. Total RNA was extracted from the three groups of samples, and the expression level of miR-122 was detected by qRT-PCR.

The mediating role of decent work perception in role clarity and job embeddedness among Chinese nurses: a cross-sectional study.

Background: The high turnover rate of the nursing team is not only detrimental to maintaining the stability of the team, but also to providing high-quality nursing services, which is an immeasurable loss for both patients and hospitals. As a predictor variable of turnover rate, increasing the level of job embeddedness among nurses can reduce their willingness to resign. Although there are currently studies reporting the correlation between role clarity, decent work perception and job embeddedness, the mechanisms between the three have not been explored, which cannot provide scientific and effective theoretical support for developing targeted measures for job embeddedness.

Machine learning: An effective tool for monitoring and ensuring food safety, quality, and nutrition.

The domains of food safety, quality, and nutrition are inundated with complex datasets. Machine learning (ML) has emerged as a powerful tool in food science, offering fast, accessible, and effective solutions compared with conventional methods. This review outlines the applications of ML in safeguarding food safety, enhancing quality, and unraveling nutrition intricacies.

Focal Adhesion Kinase Alleviates Simulated Microgravity-Induced Inhibition of Osteoblast Differentiation by Activating Transcriptional Wnt/β-Catenin-BMP2-COL1 and Metabolic SIRT1-PGC-1α-CPT1A Pathways.

The metabolic poise, or balance, between glycolysis and fatty acid oxidation (FAO) has recently been found to play a critical role in osteogenic differentiation and homeostasis. While simulated microgravity (SMG) is known to impede osteoblast differentiation (OBD) by inhibiting the Wnt/β-catenin pathway, how it affects osteoblast metabolism in this context remains unclear. We previously analyzed the effect of SMG on the differentiation of pre-osteoblast MC3T3-E1 cells and found that it reduced focal adhesion kinase (FAK) activity.

Chronic unpredictable mild stress-induced anxiety is linked to inflammatory responses and disruptions in tryptophan metabolism in male C57BL/6N mice.

Chronic stress can affect brain function through various mechanisms, leading to the development of anxiety disorders. The chronic unpredictable mild stress (CUMS) is a classic model of chronic stress. This study evaluated the effects of different durations of CUMS on anxiety-like behavior, inflammation, and tryptophan metabolism in C57BL/6N mice.

DNA methylome regulates virulence and metabolism in .

Bacterial pathogens employ epigenetic mechanisms, including DNA methylation, to adapt to environmental changes, and these mechanisms play important roles in various biological processes. is a model phytopathogenic bacterium, but its methylome is less well known than that of other species. In this study, we conducted single-molecule real-time sequencing to profile the DNA methylation landscape in three model pathovars of .