Clinical and Laboratory Features of Three Rare Chinese V210I gCJD Patients.

Genetic human prion diseases are a group of inherited encephalopathies directly associated with different mutations in PrP-encoding gene , including more than 50 different mutations worldwide. Some genotypes of mutations show ethno-correlation, and among them, genetic Creutzfeldt-Jacob disease (gCJD) with V210I mutation is frequent in European countries but rare in East Asia. Here, we comparatively analyzed the clinical and laboratory features of three Chinese patients with V210I mutant identified via the Chinese National CJD Surveillance System (CNS-CJD) in 2019.

A simple highly selective probe for discriminative visualization of endogenous cysteine, homocysteine and glutathione in living cells via three separated fluorescence channels.

Biothiols, as basic biological reactive sulfur species, perform vital actions in many critical physiological processes. Simultaneous detection and direct visualization of intracellular biothiols has great significance to figure out their metabolic mechanisms and cellular functions in living organism. However, it is an enormous challenge to selectively sense biothiols, troubled by their similar chemical structures and properties.

[The efficacy of OM85-BV in the treatment of recurrent upper respiratory tract infection with adenoid hypertrophy and the preliminary exploration of potential therapeutic mechanism].

To observe the efficacy of OM85-BV in the treatment of recurrent upper respiratory tract infection with adenoid hypertrophy and to explore its possible mechanism. Four hundred and forty-eight children with recurrent upper respiratory tract infection and adenoid hypertrophy were collected. Three hundred and twenty-six patients in the control group were treated with conventional drugs, and one hundred and twenty-two patients in the observation group were treated with OM85-BV+conventional drugs, and the treatment lasted 12 weeks.

Spatially Separating Redox Centers on Z-Scheme ZnIn S /BiVO Hierarchical Heterostructure for Highly Efficient Photocatalytic Hydrogen Evolution.

Photocatalysis technology using solar energy for hydrogen (H ) production still faces great challenges to design and synthesize highly efficient photocatalysts, which should realize the precise regulation of reactive sites, rapid migration of photoinduced carriers and strong visible light harvest. Here, a facile hierarchical Z-scheme system with ZnIn S /BiVO heterojunction is proposed, which can precisely regulate redox centers at the ZnIn S /BiVO hetero-interface by accelerating the separation and migration of photoinduced charges, and then enhance the oxidation and reduction ability of holes and electrons, respectively. Therefore, the ZnIn S /BiVO heterojunction exhibits excellent photocatalytic performance with a much higher H -evolution rate of 5.

Disentangling Multiple Effects on Excited-State Intramolecular Charge Transfer among Asymmetrical Tripartite PPI-TPA/PCz Triads.

By utilizing the bipolarity of 1,2-diphenylphenanthroimidazole (PPI), two types of asymmetrical tripartite triads (PPI-TPA and PPI-PCz) were designed with triphenylamine (TPA) and 9-phenylcarbazole (PCz). These triads are deep-blue luminescent materials with a high fluorescence quantum yield of nearly 100 %. To trace the photophysical behaviors of these triads, their excited-state evolution channels and interchromophoric interactions were investigated by ultrafast time-resolved transient absorption and excited-state theoretical calculations.

An effective vaginal gel to deliver CRISPR/Cas9 system encapsulated in poly (β-amino ester) nanoparticles for vaginal gene therapy.

Background: Gene therapy has held promises for treating specific genetic diseases. However, the key to clinical application depends on effective gene delivery.

Methods: Using a large animal model, we developed two pharmaceutical formulations for gene delivery in the pigs' vagina, which were made up of poly (β-amino ester) (PBAE)-plasmid polyplex nanoparticles (NPs) based two gel materials, modified montmorillonite (mMMT) and hectorite (HTT).

Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis.

Background: Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS.

3-year follow-up after balloon sinuplasty in children with chronic rhinosinusitis.

Objective: Balloon sinuplasty could restore nasal sinus drainage and airflow, limiting the damages and lowering the risks. This study aimed to assess the 3-year clinical efficacy, safety, and satisfactory degree of balloon sinuplasty through multiple aspects. Also the experience about treatment for postoperative adhesion was described.

Percutaneous Transforaminal Endoscopic Discectomy and Fenestration Discectomy to Treat Posterior Ring Apophyseal Fractures: A Retrospective Cohort Study.

Objective: To compare the efficacy and safety of percutaneous transforaminal endoscopic discectomy (PTED) and fenestration discectomy (FD) for posterior ring apophyseal fractures (PRAF).

Methods: This study was a retrospective cohort control study. A total of 96 patients with lumbar PRAF who underwent surgical treatment at the Henan Provincial People's Hospital of Henan University from September 2013 to December 2017 were retrospectively examined, of which 51 were treated by PTED and 45 by FD.

Ischemic postconditioning attenuates acute kidney injury following intestinal ischemia-reperfusion through Nrf2-regulated autophagy, anti-oxidation, and anti-inflammation in mice.

Intestinal ischemia-reperfusion (IIR) often occurs during and following major cardiovascular or gut surgery and causes significant organ including kidney injuries. This study was to investigate the protective effect of intestinal ischemic postconditioning (IPo) on IIR-induced acute kidney injury (AKI) and the underling cellular signaling mechanisms with focus on the Nrf2/HO-1. Adult C57BL/6J mice were subjected to IIR with or without IPo.

Stilbene Compounds Inhibit the Replications of Various Strains of Prions in the Levels of Cell Culture, PMCA, and RT-QuIC Possibly via Molecular Binding.

Resveratrol shows the ability to block prion replication in a scrapie-infected cell line, SMB-S15, and remove the infectivity of the treated cell lysates in an experimental bioassay. In this study, we compared the effectiveness of three stilbene compounds, resveratrol (Res), pterostilbene (Pte), and piceatannol (Pic), on inhibiting prion propagations in the levels of cell culture, PMCA, and RT-QuIC. All three chemicals showed active suppressions on PrP replication in SMB-S15 cells, in which Res seemed to be the most active one, followed by Pic and Pte.

Identification of causing an unexplained disease cluster in Matthews Ridge, Guyana.

Here, we report the identification of causing an unexplained disease cluster in Matthews Ridge, Guyana. In March 2019, 14 employees of Chongqing Bosai Mining Company, China, working in a manganese mining of Guyana, had unexplained fever, and two of them died. We obtained lung and brain tissues as well as the blood samples from the two deceased cases (patient No.

An Adaptive Sparse Subspace Clustering for Cell Type Identification.

The rapid development of single-cell transcriptome sequencing technology has provided us with a cell-level perspective to study biological problems. Identification of cell types is one of the fundamental issues in computational analysis of single-cell data. Due to the large amount of noise from single-cell technologies and high dimension of expression profiles, traditional clustering methods are not so applicable to solve it.

Amplified electrochemical determination of UO based on the cleavage of the DNAzyme and DNA-modified gold nanoparticle network structure.

A superior electrochemical biosensor was designed for the determination of UO in aqueous solution by integration of DNAzyme and DNA-modified gold nanoparticle (DNA-AuNP) network structure. Key features of this method include UO inducing the cleavage of the DNAzyme and signal amplification of DNA-AuNP network structure. In this electrochemical method, the DNA-AuNP network structure can be effectively modified on the surface of gold electrode and then employed as an ideal signal amplification unit to generate amplified electrochemical response by inserting a large amount of electrochemically active indicator methylene blue (MB).

Artemisinin inhibits the replication of flaviviruses by promoting the type I interferon production.

Flaviviruses are considered to be major emerging human pathogens globally. Currently available anti-flavivirus approaches are ineffective, thus there is a desperate need for broad-spectrum drugs that can be active against existing and emerging flaviviruses. Artemisinin has been found to cause an antiviral effect against several viruses; however, its antiviral effect against flaviviruses remains unexplored.

Different dietary lipid consumption affects the serum lipid profiles, colonic short chain fatty acid composition and the gut health of Sprague Dawley rats.

Palm oil (PO), leaf lard oil (LO), rapeseed oil (RO), sunflower oil (SO), and linseed oil (LINO) are the five of the most typical dietary lipids, while few studies have explored and compared their influences on the serum lipid profiles, colonic short chain fatty acids (SCFAs) composition and colon health of Sprague Dawley (SD) rats. Results from the present work showed that PO and LO groups showed significantly higher serum TG and TC level compared with Ctrl group, whereas, the LDL-C/HDL-C and TC/HDL-C ratio were significantly lower in the RO, SO and LINO groups. Different dietary lipid consumption (15% of the normal diet) decreased the colonic SCFAs concentration.

Cinobufacini Inhibits Colon Cancer Invasion and Metastasis via Suppressing Wnt/β-Catenin Signaling Pathway and EMT.

Cinobufacini is a well-known Chinese medicine extracted from Venenum Bufonis, also called Chan Su. It has been used clinically for various cancers, including colon cancer. However, the function of Cinobufacini on colon cancer invasion and metastasis, and its underlying molecular mechanism, is still not clear.

IP10, KC and M-CSF Are Remarkably Increased in the Brains from the Various Strains of Experimental Mice Infected with Different Scrapie Agents.

Activation of inflammatory cells and upregulations of a number of cytokines in the central nervous system (CNS) of patients with prion diseases are frequently observed. To evaluate the potential changes of some brain cytokines that were rarely addressed during prion infection, the levels of 17 different cytokines in the brain homogenates of mice infected with different scrapie mouse-adapted agents were firstly screened with Luminex assay. Significant upregulations of interferon gamma-induced protein 10 (IP10), keratinocyte chemoattractant (KC) and macrophage colony stimulating factor (M-CSF) were frequently detected in the brain lysates of many strains of scrapie infected mice.

In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.

Sustained infection of high-risk human papillomavirus (HR-HPVs), especially HPV16 and HPV18, is a major cause of cervical cancer. E6 and E7 oncoproteins, encoded by the HPV genome, are critical for transformation and maintenance of malignant phenotypes of cervical cancer. Here, we used an emerging programmable clustered regularly interspaced short palindromic repeat (CRISPR)/Cas13a system to cleave HPV 16/18 E6/E7 messenger RNAs (mRNAs).

Role of adiponectin in diabetes myocardial ischemia-reperfusion injury and ischemic postconditioning.

Purpose: Patients with diabetes are vulnerable to myocardial I/R (ischaemia/reperfusion) injury, but are not responsive to IPO (ischaemic post-conditioning). We hypothesized that decreased cardiac Adiponectin (APN) is responsible for the loss of diabetic heart sensitivity to IPO cardioprotecton.

Methods: Diabetic rats were subjected to I/R injury (30 min of LAD occlusion followed by 120 min of reperfusion).

Ultrathin sulfate-intercalated NiFe-layered double hydroxide nanosheets for efficient electrocatalytic oxygen evolution.

As an important two-dimensional material, layered double hydroxides (LDHs) show considerable potential in electrocatalytic reactions. However, the great thickness of the bulk LDH materials significantly limits their catalytic activity. In this work, we report ultrathin NiFe-LDH nanosheets with sulfate interlayer anions (NiFe(SO)(OH)·7HO) (U-LDH(SO )), which can be synthesized in gram-scale by a simple solvothermal method.

Characterization of the immunomodulatory properties of alveolar bone-derived mesenchymal stem cells.

Background: Recently, mesenchymal stem cells (MSCs) have been shown to have immunomodulatory properties which hold promise for their clinical use to treat inflammatory conditions. Relative to bone marrow-derived MSCs (BMSCs), which are typically isolated from the iliac crest, we have recently demonstrated that MSCs can be predictably isolated from the alveolar bone (aBMSCs) by less invasive means. As such, the aim of this study was to characterize the immunomodulatory properties of aBMSCs relative to BMSCs.