Enhanced Resistance to Pokkah Boeng Disease in Sugarcane Through Host-Induced Gene Silencing Targeting FsCYP51 in Fusarium sacchari.

Pokkah boeng disease (PBD), a common and highly destructive disease of sugarcane, is mainly caused by Fusarium sacchari. Breeding sugarcane resistant to PBD is challenging due to the limited availability of immune or highly resistant germplasm resources. Host-induced gene silencing (HIGS) based on RNA interference (RNAi) is a promising disease-control method that offers strong disease-targeting ability with low environmental impact.

Mitochondrial genome structural variants and candidate cytoplasmic male sterility-related gene in sugarcane.

Background: Sugarcane is a crucial crop for both sugar and bioethanol production. The nobilization breeding and utilization of wild germplasm have significantly enhanced its productivity. However, the pollen sterility in Saccharum officinarum restricts its role to being a female parent in crosses with Saccharum spontaneum during nobilization breeding, resulting in a narrow genetic basis for modern sugarcane cultivars.

The highly allo-autopolyploid modern sugarcane genome and very recent allopolyploidization in Saccharum.

Modern sugarcane, a highly allo-autopolyploid organism, has a very complex genome. In the present study, the karyotype and genome architecture of modern sugarcane were investigated, resulting in a genome assembly of 97 chromosomes (8.84 Gb).

mA demethylase CpALKBH regulates mRNA stability to modulate the development and virulence of chestnut blight fungus.

Unlabelled: As the most abundant eukaryotic mRNA modification, N-methyladenosine (mA) plays a crucial role in regulating multiple biological processes. This methylation is regulated by methyltransferases and demethylases. However, the regulatory role and mode of action of mA demethylases in fungi remain poorly understood.

New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.

Persistent plant viruses are widespread in natural ecosystems. However, little is known about why persistent infection with these viruses may cause little or no harm to their host. Here, we discovered a new polerovirus that persistently infected wild rice plants by deep sequencing and assembly of virus-derived small-interfering RNAs (siRNAs).

Functional Characterization of the Gibberellin (GA) Receptor ScGID1 in Sugarcane.

Sugarcane smut caused by represents the most destructive disease in the sugarcane industry, causing host hormone disruption and producing a black whip-like sorus in the apex of the stalk. In this study, the gibberellin metabolic pathway was found to respond to infection, and the contents of bioactive gibberellins were significantly reduced in the leaves of diseased plants. The gibberellin receptor gene was identified and significantly downregulated.

Surface pCO and air-water CO fluxes dominated by submerged aquatic vegetation: Implications for carbon flux in shallow lakes.

Inland lakes are crucial for processing, storing, and releasing carbon dioxide (CO), and they play a significant role in the global carbon cycle and climate change. Studies have shown that inland lakes are mostly supersaturated in CO, making them significant sources to the atmosphere. However, estimating CO fluxes from inland lakes is still challenging due to large variations in surface water CO partial pressure (pCO).

VirB11, a traffic ATPase, mediated flagella assembly and type IV pilus morphogenesis to control the motility and virulence of Xanthomonas albilineans.

Xanthomonas albilineans (Xal) is a gram-negative bacterial pathogen responsible for developing sugarcane leaf scald disease, which engenders significant economic losses within the sugarcane industry. In the current study, homologous recombination exchange was carried out to induce mutations within the virB/D4-like type IV secretion system (T4SS) genes of Xal. The results revealed that the virB11-deletion mutant (ΔvirB11) exhibited a loss in swimming and twitching motility.

Diffusible Signal Factor-Mediated Quorum Sensing Modulates Swarming in .

spp. are plant pathogens known for significantly impacting crop yields. Among them, () is notable for colonizing the xylem and causing sugarcane leaf scald disease.

N6-methyladenosine RNA methyltransferase CpMTA1 mediates CpAphA mRNA stability through a YTHDF1-dependent m6A modification in the chestnut blight fungus.

In eukaryotic cells, N6-methyladenosine (m6A) is the most prevalent RNA epigenetic modification that plays crucial roles in multiple biological processes. Nevertheless, the functions and regulatory mechanisms of m6A in phytopathogenic fungi are poorly understood. Here, we showed that CpMTA1, an m6A methyltransferase in Cryphonectria parasitica, plays a crucial role in fungal phenotypic traits, virulence, and stress tolerance.

Mosquitocidal toxin-like islands in Bacillus thuringiensis S2160-1 revealed by complete-genome sequence and MS proteomic analysis.

Here, we present the whole genome sequence of Bt S2160-1, a potential alternative to the mosquitocidal model strain, Bti. One chromosome genome and four mega-plasmids were contained in Bt S2160-1, and 13 predicted genes encoding predicted insecticidal crystal proteins were identified clustered on one plasmid pS2160-1p2 containing two pathogenic islands (PAIs) designed as PAI-1 (Cry54Ba, Cry30Ea4, Cry69Aa-like, Cry50Ba2-like, Cry4Ca1-like, Cry30Ga2, Cry71Aa-like, Cry72Aa-like, Cry70Aa-like, Cyt1Da2-like and Vpb4C1-like) and PAI-2 (Cyt1Aa-like, and Tpp80Aa1-like). The clusters appear to represent mosquitocidal toxin islands similar to pathogenicity islands.

, an ortholog of small ubiquitin-like modifier, is essential for growth, organelle function, virulence, and antiviral defense in .

Introduction: SUMOylation is an important post-translational modification that regulates the expression, localization, and activity of substrate proteins, thereby participating in various important cellular processes such as the cell cycle, cell metabolism, gene transcription, and antiviral activity. However, the function of SUMOylation in phytopathogenic fungi has not yet been adequately explored.

Methods: A comprehensive analysis composed of proteomics, affinity pull-down, molecular and cellular approaches was performed to explore the roles of SUMOylation in , the fungal pathogen responsible for chestnut blight.

Fusarium sacchari hypovirus 1, a Member of with Virulence Attenuation Capacity in Phytopathogenic Species.

In a survey of mycoviruses in species that cause sugarcane Pokkah boeng disease, twelve strains from three species (, , and ) were found to contain Fusarium sacchari hypovirus 1 (FsHV1), which we reported previously. The genomes of these variants range from 13,966 to 13,983 nucleotides, with 98.6% to 99.

Redundant and Distinct Roles of Two 14-3-3 Proteins in , Pathogen of Sugarcane Pokkah Boeng Disease.

, a key pathogen of sugarcane, is responsible for the Pokkah boeng disease (PBD) in China. The 14-3-3 proteins have been implicated in critical developmental processes, including dimorphic transition, signal transduction, and carbon metabolism in various phytopathogenic fungi. However, their roles are poorly understood in This study focused on the characterization of two 14-3-3 protein-encoding genes, and , within .

FsCGBP, a Cutinase G-Box Binding Protein, Regulates the Growth, Development, and Virulence of the Pathogen of Sugarcane Pokkah Boeng Disease.

is a causal agent of sugarcane Pokkah boeng, an important fungal disease that causes a considerable reduction in yield and sugar content in susceptible varieties of sugarcane worldwide. Despite its importance, the fungal factors that regulate the virulence of this pathogen remain largely unknown. In our previous study, mapping of an insertional mutant defect in virulence resulted in the identification of a cutinase G-box binding protein gene, designated that encodes a C2H2-type transcription factor (TF).

An efficient inoculation method to evaluate virulence differentiation of field strains of sugarcane smut fungus.

Sugarcane smut, caused by the fungal pathogen , is a prominent threat to the sugarcane industry. The development of smut resistant varieties is the ultimate solution for controlling this disease, due to the lack of other efficient control methods. Artificial inoculation method is used to evaluate the virulence differentiation of pathogens.

A chromosomal-scale genome assembly of modern cultivated hybrid sugarcane provides insights into origination and evolution.

Sugarcane is a vital crop with significant economic and industrial value. However, the cultivated sugarcane's ultra-complex genome still needs to be resolved due to its high ploidy and extensive recombination between the two subgenomes. Here, we generate a chromosomal-scale, haplotype-resolved genome assembly for a hybrid sugarcane cultivar ZZ1.

The P2 nucleic acid binding protein of is a viral pathogenic factor.

Background: . is the primary source of sugar and plays a significant role in global renewable bioenergy. (SCBV) is one of the most important viruses infecting sugarcane, causing severe yield losses and quality degradation.

Small secreted effector protein from Fusarium sacchari suppresses host immune response by inhibiting ScPi21-induced cell death.

Fusarium sacchari is one of the primary pathogens causing pokkah boeng disease, which impairs the yield and quality of sugarcane around the world. Understanding the molecular mechanisms of the F. sacchari effectors that regulate plant immunity is of great importance for the development of novel strategies for the persistent control of pokkah boeng disease.

Characterization of controlling factors for soil organic carbon stocks in one Karst region of Southwest China.

Soil organic carbon (SOC) contributes the most significant portion of carbon storage in the terrestrial ecosystem. The potential for variability in carbon losses from soil can lead to severe consequences such as climate change. While extensive studies have been conducted to characterize how land cover type, soil texture, and topography impact the distribution of SOC stocks across different ecosystems, little is known about in Karst Region.

Identification of common fungal extracellular membrane (CFEM) proteins in that inhibit plant immunity and contribute to virulence.

Common fungal extracellular membrane (CFEM) domain-containing protein has long been considered an essential effector, playing a crucial role in the interaction of pathogens and plant. Strategies aimed at understanding the pathogenicity mechanism of are eagerly anticipated to ultimately end the spread of pokkah boeng disease. Twenty FsCFEM proteins in the genome of have been identified, and four FsCFEM effector proteins have been found to suppress BCL2-associated X protein-triggered programmed cell death in .