[Verification of the safety, inoculability, reactogenicity and antigenic properties of a live recombinant smallpox-hepatitis B vaccine in an experiment in volunteers].

Trials of the first Soviet live recombinant smallpox-hepatitis B vaccine (SHBV) in volunteers (20 men aged 18-20 years) showed its safety, good "take"-rate, and lower reactogenicity as compared with the standard smallpox vaccine (LIVP strain). Smallpox virus-neutralizing antibodies in response to SHBV were produced as well as in response to the smallpox vaccine. Revaccination of human subjects with smallpox vaccine and SHBV 45 days after the previous vaccination resulted in antibody booster to vaccinia virus.

[Heterogeneous distribution of influenza A matrix protein in polyacrylamide gel, detected using an immunoblotting method with monoclonal antibodies].

The immune blotting method using monoclonal antibodies to Ml protein showed protein Ml to migrate in several bands in polyacrylamide gel electrophoresis (PGE) of influenza A virus polypeptides. The heterogeneous distribution of Ml protein in PGE is due to the formation of aggregates: dimers, trimers, and polymers of a higher order. The capacity of Ml protein for aggregation is typical not of all influenza virus strains and most likely is not associated with gel overloading.

[Analysis of antibody formation to the vaccinia virus in human subjects and rabbits in response to the administration of a recombinant vaccinia-hepatitis B vaccine].

Immune blotting analysis was used to study antibody production to virion proteins of vaccinia virus (VV) in response to inoculation of human volunteers and rabbits with VV (LIVP strain) and its genetic engineering recombinant variants carrying a gene of hepatitis B virus surface antigen (LIOGEN-HB/C2-TK-) and, plus to that, the TK gene of herpes simplex virus (LIOGEN-HB/C2-TK+). In the blood of human subjects vaccinated 10-15 years earlier antibodies to many virion proteins were demonstrated: not only to surface 42K, 35K, 11K, but also to nucleoid proteins 135K, 88K, 62K, 60K, 26K. Vaccination with genetic engineering viruses results in stimulation of synthesis of antibodies to VV virion proteins.

[Dynamics of the formation of antibodies to surface and internal proteins of the herpes simplex virus in a model experiment on rabbits].

It has been shown by immunoblotting that antibodies to HSV-1 nucleocapsid proteins (39-45K) predominate at the early stages of infection (up to day 21 after infection) in rabbits. At later stages (up to day 75) the intensity of bands corresponding to virus-specific glycoproteins (presumably gD, gE, gC) increases. At the same time the level of antibodies to nucleocapsid proteins diminishes.

[Chronic rubella virus-induced infection of human continuous cells].

A stable chronically rubella virus-infected culture of HEp-2-BK cells existing for over 30 months has been obtained as a result of a single inoculation and further passages. This system is characterized by the lack of cell destruction and permanent production of infectious virus in titres of 2.5-6.

[Virus-specific RNA synthesis in a cell culture in acute and chronic infection with the tick-borne encephalitis virus].

Virus particles produced in acute and chronic infection of cell cultures with tick-borne encephalitis virus (TBE) and examined by centrifugation in sucrose density gradient had the buoyant density of 1.19 g/ml and sedimentation constant 290 S. Studies of the synthesis of virus-specific RNAs in the cytoplasm of TBE virus chronically infected cells revealed synthesis of all RNA classes typical of TBE virus reproduction in acutely infected cells.

[Physico-chemical properties of deoxyribonucleoprotamine from sturgeon sperm heads].

Deoxyribonucleoprotamine (DNPn) from sonicated nuclei of sturgeon sperm heads was studied by means of ring dichroism. A derivative analysis of DNA and DNPn melting curves in 1 mM Tris. HCl pH 8.