Publications by authors named "Chee Kong Yap"

Article Synopsis
  • Antifouling biocides, including metals like Cu and Zn, prevent organisms from attaching to ship hulls but are toxic to marine life, prompting a study of microparticles (MPs) in Osaka Bay.
  • Seven types of MPs containing various metals were identified, with most being acrylic resins linked to antifouling paints, revealing high concentrations of Cu and Zn in the samples analyzed.
  • The research utilized X-ray fluorescence spectroscopy to accurately measure the metal concentrations in the MPs by establishing calibration curves based on standard paint particles.
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  • Phenytoin, an antiepileptic drug, was studied for its impact on the swimming behavior and reproductive ability of Japanese medaka fish, revealing significant behavioral abnormalities and reduced reproductive success.
  • Abnormal swimming behaviors included imbalance and rotation, but these improved after discontinuing phenytoin exposure.
  • The drug also suppressed key reproductive gene expressions related to the kisspeptin system, particularly kiss1, which likely contributed to the females' lack of interest in mating and reduced spawning.
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Phthalate and non-phthalate plasticizers are used in polymer materials, such as plastic and rubber. It has recently been found that diisobutyl adipate (DIBA), which is considered an environmentally safe non-phthalate plasticizer, potentially acts as a thyroid disruptor in fish. Here, we investigated the sexual hormone effects of DIBA based on the expression levels of genes that respond to endocrine disruption and sexual hormone activity in the livers and gonads, and on gonadal sexual differentiation in Japanese medaka.

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With the expected colonization of human daily life by artificial intelligence, including in industry productivity, the deployment of Industry 4.0 (I4) in the food agriculture industry (FAI) is expected to revolutionize and galvanize food production to increase the efficiency of the industry's production and to match, in tandem, a country's gross domestic productivity. Based on a literature review, there have been almost no direct relationships between the I4-Food-Agriculture (I4FA) Nexus and the agroecosystem.

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The abundance, distribution, and composition of microparticles (MPs) in the sea-surface microlayer (S-SML, less than 100 μm of sea surface in this experiment) and in bulk water (1 m under the sea surface) were investigated to evaluate the pollution level of MPs in Osaka Bay in Japan. Both seawater fractions were collected at eight sites including ship navigation routes, the coastal area, and the center of Osaka Bay for 2021-2023. MPs were filtered for four size ranges (10-53, 53-125, 125-500, and >500 μm) and then digested with HO.

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The primary objective of the study was to examine the distribution of various elements, namely Cadmium (Cd), Copper (Cu), Iron (Fe), Nickel (Ni), and Lead (Pb), in the soft tissues, shells, and associated surface sediments of () mangrove snails collected from Sungai Besar Sepang. To conduct the analysis, the preferred and most convenient methods employed were Instrumental Neutron Activation Analysis (INAA) and Atomic absorption spectrometry (AAS). The results showed that the mean concentration of elements in the sediments and soft tissues followed the order Fe > Cu > Ni > Pb > Cd, while for the shell of , it was Fe > Ni > Cu > Pb > Cd.

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The present field-based study aimed to determine the levels of six potentially toxic metals (PTM)s (Cd, Cu, Fe, Ni, Pb, and Zn determined using a flame atomic-absorption spectrophotometer) using transplanted green-lipped mussel from a polluted site at Kampung Pasir Puteh (KPP) to unpolluted sites at Kampung Sungai Melayu (KSM) and Sungai Belungkor (SB) in the Johore Straits (SOJ), and to estimate the human health risks of the PTMs after the depuration periods. Interestingly, after 10 weeks of depuration in the two unpolluted sites, there were 55.6-88.

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This paper aims to review the literature on 'Effective Microorganism (EM)' and 'Fertilizer' from the Scopus database and to discuss EMs using -based sources for biofertilizer production from socio economic insights. Based on EM and fertilizer publications on the Scopus database, all the 17 papers reviewed provided no detailed information on the -status of the biofertilizers inoculated with EM. The impacts of -certified biofertilizers will trigger the certification in food products by (a) catering for the increasing food demand due to expectedly Muslim population expansion, (b) contributing to the sustainable buying behaviour of products' consumers in the future, (c) catering for the increasing number of Muslim travellers around the world, (d) becoming a positive driver for higher production of more foods that can enhance food safety, human health and well-being, and (e) creating a cost-effective and increasing food marketability.

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The present study aimed to assess the human health risks of six potentially toxic metals (PTMs) (Cd, Cu, Fe, Ni, Pb and Zn) in 21 populations of popular mangrove snails, , collected from Malaysia. In general, the concentrations (mg/kg wet weight) of Cd (0.03-2.

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The present study aimed to confirm the use of the byssus (BYS) of the green-lipped mussel as a biomonitoring biopolymer for zinc (Zn) by comparing it to copper (Cu) and cadmium (Cd) pollution in coastal waters under experimental field conditions, based on the transplantation of caged mussels between polluted and unpolluted sites in the Straits of Johore (SOJ). Four important evidential points were found in the present study. First, the 34 field-collected populations with BYS/total soft tissue (TST) ratios > 1 indicated that the BYS was a more sensitive, concentrative, and accumulative biopolymer for the three metals than TST.

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  • Pyriproxyfen is a pesticide that affects zebrafish growth and thyroid gene expression during their early life stages, showing a lethal effect at higher concentrations.
  • The study found that while certain thyroid hormone-related gene expressions decreased with lower exposures, others increased at higher levels, indicating it disrupts thyroid hormone activity.
  • Zebrafish growth was inhibited by pyriproxyfen, mainly due to the suppression of growth hormone (gh) expression, while insulin-like growth factor-I (igf-1) levels did not change.
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  • This study investigated the levels of potentially toxic metals (Cu, Ni, Pb, Zn) in surface sediments of the Klang mangrove ecosystem and examined the ability of Avicennia officinalis to help clean up these metals through phytoremediation.
  • The concentrations of the metals varied, with Cu posing a moderate ecological risk while Ni, Pb, and Zn were deemed to have low potential ecological risks; all the metals were found to be non-carcinogenic according to the hazard index.
  • The research identified specific parts of the Avicennia officinalis plant that are more effective in removing essential and non-essential metals, providing valuable data to manage and protect the ecological health of the Klang mangrove ecosystem.
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Plasticizer pollution of the water environment is one of the world's most serious environmental issues. Phthalate plasticizers can disrupt endocrine function in vertebrates. Therefore, this study analyzed thyroid-related, reproduction-related, and estrogen-responsive genes in Japanese medaka (Oryzias latipes) to determine whether non-phthalate diisobutyl adipate (DIBA) plasticizer could affect endocrine hormone activity or not.

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  • Water pollution from plasticizers, particularly phthalates, poses a significant environmental threat as they can disrupt endocrine systems in animals.
  • This study focused on the non-phthalate plasticizer DEHS and its effects on gene expression related to thyroid hormones and reproduction in Japanese medaka fish, finding that DEHS suppressed certain thyroid-related gene expression and negatively impacted swimming performance.
  • The research represents the first evidence that DEHS can interfere with thyroid-related metabolism in fish, while not significantly affecting reproduction-related gene expression.
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Pollution of water bodies with plasticizers is a serious environmental problem worldwide. In this study, we investigated the effects of plasticizers bis-(2-ethylhexyl) phthalate (DEHP) and bis-(2-ethylhexyl) adipate (DEHA) in Japanese medaka (Oryzias latipes). DEHP significantly increased the expression of all the genes tested: thyroid stimulating hormone beta subunit (tshβ-like), tshβ, deiodinase 1 (dio1), deiodinase 2 (dio2), and thyroid hormone receptor alpha (trα) and beta (trβ).

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Pollution characteristics and associated ecological risks of PAHs in sediments from Brunei Bay, Brunei were investigated. The concentrations of ∑16 PAHs ranged from 826.7 to 2955.

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In recent decades the development of desalination plants (DPs) for desalination of seawater has increased dramatically, while little attention has been paid to the effects of this activity on the accumulation of heavy metals (HMs) in the sediments of affected ecosystems. The present study was implemented to evaluate (1) heavy metal accumulation in sediments impacted by DPs discharges, (2) spatial and temporal changes of HMs and the contamination degree by different types of pollution indexes (single and integrated indices), and (3) ecological risk assessment of cadmium (Cd), lead (Pb), zinc (Zn) and copper (Cu) in sediments affected by DPs discharges. A total of 288 sediment samples were collected seasonally at 24 stations from November 2019 to October 2020.

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Human health risk and phytoremediation of potentially toxic metals (PTMs) in the edible vegetables have been widely discussed recently. This study aimed to determine the concentrations of four PTMs, namely Cd, Fe, Ni, and Zn) in Amaranthus viridis (leaves, stems, and roots) collected from 11 sampling sites in Peninsular Malaysia and to assess their human health risk (HHR). In general, the metal levels followed the order: roots > stems > leaves.

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  • - This study evaluated the health risks of nickel (Ni) exposure from consuming certain marine fish and mollusks in Setiu, Terengganu, Malaysia, focusing on 19 fish species and two types of seafood: mangrove snails and cockles.
  • - Nickel levels in these seafood types were found to be below the maximum permissible limit, and the risk evaluations indicated that Ni's target hazard quotient values were less than 1.00 for Malaysian consumers, suggesting no significant non-carcinogenic risk from seafood consumption.
  • - The estimated weekly intake of nickel from these seafood sources was also below the established safe limits, concluding that regular consumption of these seafood options would not harmfully impact consumer health regarding nickel exposure.
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Potentially toxic metals (PTMs) in edible marine fish have been widely reported from at least 15 different regions or countries in the literature. This evidently demonstrates the importance of monitoring the PTMs in fish fillets from a human health risk (HHR) point of view. This study aims to assess the HHR of Cu and Zn in 19 species of marine fish from popular marine fish loading sites at Setiu in Terengganu, on the east coast of Peninsular Malaysia, collected between August 2016 and February 2017.

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The coral health of Pulau Anak Datai (PAD), located off the northwest of Langkawi, Malaysia, was assessed using the Coral Health Index (CHI) method. Three ecological parameters, namely, benthic cover, fish biomass, and microbes (Vibrio) were determined at four sites around the island in 2019. In addition, community parameters such as coral mortality index, coral richness, relative abundance, diversity index, Evenness tests, and reef morphology were measured for each site.

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  • The ASEAN-5 countries are becoming major economic hubs in Asia but face increasing environmental concerns due to heavy metal pollution, particularly in aquatic sediments.
  • A review of studies from 1981 to 2021 revealed high concentrations of copper (Cu), lead (Pb), and zinc (Zn) in these sediments, linked to areas with significant human activity.
  • Ecological risk assessments showed varied risk levels for these metals, with many sites posing 'considerable potential ecological risks,' particularly one location in the Philippines with very high Cu levels due to mining activities.
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Human activities due to different land uses are being studied widely in many countries. This study aimed to determine the ecological risks and human health risk assessments (HHRA) of Cd, Pb, Ni, Cu, and Zn in the topsoils of six land uses in Peninsular Malaysia. The ranges of the potentially toxic metals (PTMs) in the soils (mg/kg, dry weight) of this study were 0.

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