Analogue-Sensitive Inhibition of Histone Demethylases Uncovers Member-Specific Function in Ribosomal Protein Synthesis.

Lysine demethylases (KDMs) catalyze the oxidative removal of the methyl group from histones using earth-abundant iron and the metabolite 2-oxoglutarate (2OG). KDMs have emerged as master regulators of eukaryotic gene expression and are novel drug targets; small-molecule inhibitors of KDMs are in the clinical pipeline for the treatment of human cancer. Yet, mechanistic insights into the functional heterogeneity of human KDMs are limited, necessitating the development of chemical probes for precision targeting.

Individualized Treatment Approach for Rectal Adenocarcinoma in the Setting of Congenital Neutropenia.

Congenial neutropenia is a rare genetic disorder that puts individuals at risk of life-threatening bacterial infections early in life, and the current standard of care includes the use of colony-stimulating factors or curative intent bone marrow transplant. Cancer treatment strategies that include surgery, chemotherapy, radiation, and immunotherapy present significant challenges to an individual with a baseline immunodeficiency as seen in this condition. Evidence-based national guidelines aid physicians and patients in moving through complex cancer care regimens.

Dance practice modifies functional fitness, lipid profile, and self-image in postmenopausal women.

Objective: The aim of the present study was to analyze the effects of dance practice on body composition, metabolic profile, functional fitness, and self-image/self-esteem in postmenopausal women.

Methods: A total of 36 postmenopausal participants (mean age 57 years) danced three times per week for 90 minutes each day and were evaluated before and after 16 weeks. The parameters evaluated were body composition (body fat and lean mass), blood lipids, functional fitness, self-image, and self-esteem.

Minimal clinical benefit of single agent Orathecin (Rubitecan) in heavily pretreated metastatic breast cancer.

Purpose: The purpose of this phase II study was to evaluate the efficacy and tolerability of Orathecin, an oral camptothecin analog that has exhibited antitumor activity in breast cancer patients during preclinical studies.

Methods: Sixteen patients with metastatic breast cancer previously treated with anthracycline and taxane were utilized in the study. Orathecin was administered orally at 1.

Effect of 0.02% NaF solution on enamel demineralization and fluoride uptake by deciduous teeth in vitro.

The application of 0.02% NaF solution on teeth with a cotton swab instead of brushing with fluoride dentifrice has been suggested for young children to reduce the risk of dental fluorosis, but its anticariogenic effect has not been evaluated. Thus, we studied the in vitro effect of 0.

Visual Ability Score -- a new method to analyze ability in visually impaired children.

Purpose: We analyzed the correlation between the Preferential Looking (PL) acuities and the Visual Ability Scores (VAS) of 600 patients (many with severe retinopathy of prematurity) to determine their ability to perform various activities within the daily environment.

Methods: Visual acuity was measured by PL. Sixteen visual activities within the environment were analyzed.

Five years' experience with the Grating disc. A simplified method of evaluating vision in young children.

The Grating Disc (GD) is a simplified visual test designed for children 18 months to 4 approximately 5 years. Two hundred pediatric patients (103 males, 97 females; aged 5 months to > 6 years) participated in this study to determined how the GD visual acuity (VA) correlates with the Preferential Looking (PL) VA. Forty-six percent of the patients had varying degrees of retinopathy of prematurity.

Correlating preferential looking visual acuity and visual behavior in severely visually handicapped children.

We developed a series of questions that reflects the functional abilities of severely visually handicapped children. The study population comprised 100 patients aged 30 to 60 months (mean 43.23), mostly afflicted with retinopathy of prematurity.

Comparison among different ovarian stimulation regimens for assisted procreation procedures in patients with endometriosis.

The objective of our study was to establish the most adequate ovarian stimulation regimen for assisted procreation in endometriotic patients. It consisted of a retrospective analysis comparing the use of the gonadotrophin-releasing hormone analogue (GnRHa) buserelin either for 3 months or for 3 weeks and continued with ovarian stimulation with human menopausal gonadotrophin (HMG), and the use of clomiphene citrate in association with HMG for in-vitro fertilization (IVF) and embryo transfer, gamete intra-Fallopian transfer (GIFT) and zygote intra-Fallopian transfer (ZIFT). A total of 145 patients with endometriosis in 174 cycles were divided into two groups according to the revised American Fertility Society staging of the disease (group A, stages 1 and 2; group B, stages 3 and 4).

Effects of cryopreservation on survival and development of interphase- and mitotic-stage 1-cell mouse embryos.

The effects of cryopreservation with 1,2-propanediol on two groups of 1-cell mouse embryos were studied in terms of survival after thawing, growth in vitro until the blastocyst stage and development in vivo assessed by the number of implantations and living fetuses. The two groups were divided according to different stages in the cell cycle: cells in (i) interphase with two distinct pronuclei or (ii) mitosis just prior to the first cleavage division. Zygotes in the interphase stage proved to be more resistant to freezing and thawing procedures, showing a significantly higher survival rate after thawing than zygotes in mitosis (78.