A universal approach to gene expression engineering.

In this study, we provide a universal approach to Gene Expression Engineering (GeneEE) for creating artificial expression systems. GeneEE leads to the generation of artificial 5 regulatory sequences (ARES) consisting of promoters and 5 untranslated regions. The ARES lead to the successful recruitment of RNA polymerase, related sigma factors and ribosomal proteins that result in a wide range of expression levels.

Complete Genome Sequence of the Phage Ayreon.

We report the whole-genome sequence of a new temperate phage, Ayreon, comprising a linear double-stranded DNA (dsDNA) genome of 44,708 bp.