Objectives: This study investigates the neuropharmacologic properties of Scopoletin, a bioactive compound in (EA) extract, for managing cognitive impairment using , , and zebrafish embryo toxicity assays.
Methods: The study estimates Scopoletin concentration in EA extract using HPTLC, assesses antioxidant properties using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ability of plasma (FRAP) assays, and uses bioinformatic tools for scopoletin targets. Zebrafish embryo toxicity (ZET) is used to assess its toxicological profile.