Publications by authors named "Chayan Bhattacharya"
Substrate DNA length regulates the activity of TET 5-methylcytosine dioxygenases.
Cell Biochem Funct
August 2023
The ten-eleven translocation (TET) isoforms (TET1-3) play critical roles in epigenetic transcription regulation. In addition, mutations in the TET2 gene are frequently detected in patients with glioma and myeloid malignancies. TET isoforms can oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, by iterative oxidation.
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- Cytosine methylation (5mC) is important for regulating gene expression during the development of organisms and is affected by enzymes known as TET dioxygenases, which facilitate the process of active demethylation.
- TET2, a member of the TET family, converts 5mC into various modified forms, and mutations in TET2 are often found in certain blood cancers.
- This study introduces a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to separate and quantify these modified cytosines, marking a significant advance in understanding their biological functions.
The epigenetic transcription regulation mediated by 5-methylcytosine (5mC) has played a critical role in eukaryotic development. Demethylation of these epigenetic marks is accomplished by sequential oxidation by ten-eleven translocation dioxygenases (TET1-3), followed by the thymine-DNA glycosylase-dependent base excision repair. Inactivation of the TET2 gene due to genetic mutations or by other epigenetic mechanisms is associated with a poor prognosis in patients with diverse cancers, especially hematopoietic malignancies.
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