The Concise Language Paradigm (CLaP), a framework for studying the intersection of comprehension and production: electrophysiological properties.

Studies investigating language commonly isolate one modality or process, focusing on comprehension or production. Here, we present a framework for a paradigm that combines both: the Concise Language Paradigm (CLaP), tapping into comprehension and production within one trial. The trial structure is identical across conditions, presenting a sentence followed by a picture to be named.

A flexible class of generalized joint frailty models for the analysis of survival endpoints.

This article focuses on shared frailty models for correlated failure times, as well as joint frailty models for the simultaneous analysis of recurrent events (eg, appearance of new cancerous lesions or hospital readmissions) and a major terminal event (typically, death). As extensions of the Cox model, these joint models usually assume a frailty proportional hazards model for each of the recurrent and terminal event processes. In order to extend these models beyond the proportional hazards assumption, our proposal is to replace these proportional hazards models with generalized survival models, for which the survival function is modeled as a linear predictor through a link function.

Left Ventricular Outflow Tract Obstruction in Patients Treated With Milrinone for Cerebral Vasospasm: Case Report and Literature Review.

Subarachnoid hemorrhage is associated with high morbidity and mortality, and cerebral arterial vasospasm is one of its main complications that determines neurological prognosis. The use of intravenous milrinone is becoming more common in the treatment of vasospasm. This molecule has positive inotropic and vasodilating properties by inhibiting phosphodiesterase-3.

Sensitivity of Capillary Electrophoresis to Detect Monoclonal Immunoglobulins: a Report of Two Cases and a Systematic Review.

Background: The detection of monoclonal immunoglobulins (MIg) is a key element in the diagnosis of monoclonal gammopathies.

Methods: Here we report two cases of high concentration serum IgM-kappa MIgs (6.4 g/L and 6.

Factors associated with poor fetal outcome in placental abruption.

Objectives: We aimed at describing placental abruption in our county and at evaluating factors associated with poor fetal outcome.

Study Design: In this case-control study, women with placental abruption were identified from two databases of Brest University Hospital between January 2013 and December 2018.

Main Outcome Measures: Placental histological findings, course of pregnancies, maternal and fetal characteristics were described and compared between cases (placental abruption with stillbirth or neonatal death) and controls.

Utility of changes in end-tidal carbon dioxide after volume expansion to assess fluid responsiveness in the operating room: a prospective observational study.

Background: From a physiological viewpoint, changes in end-tidal carbon dioxide (EtCO) could be a simple, noninvasive, and inexpensive way to monitor changes in cardiac index. This study aimed to assess the utility of changes in EtCO as a marker of fluid responsiveness after volume expansion in the operating room.

Methods: A prospective observational study was conducted in a tertiary university teaching hospital, from August 2018 to February 2019.

Correlation of biological parameters with placental parameters and pregnancy outcomes in pre-eclamptic women.

Introduction: Pre-eclampsia is characterized by maternal vascular malperfusion and chronic inflammation in placenta. Our purpose was to investigate the potential correlation of biological parameters with placental parameters and pregnancy outcomes in pre-eclamptic women.

Methods: Pre-eclamptic women were identified by interrogation of the Medical Registry Department in six French maternities between April 2013 and June 2018.

Comparison of the New, Rapid, and Fully Automated Kryptor TSH receptor Antibodies Assay (B.R.A.H.M.S.) with the Radioimmunological Assay (B.R.A.H.M.S.).

Background: Radioimmunoassays, which are often not automated and time-consuming, are gradually being re-placed in medical laboratories by non-radioactive methods that need to be evaluated. The purpose was to compare the measurement of thyroid-stimulating hormone receptor antibodies (TRAb) by the new Brahms' kit using Kryptor TRACE technology and the Brahms' radioimmunoassay.

Methods: We prospectively collected all samples from patients who received thyroid-stimulating hormone receptor antibodies testing in July 2018 at the University Hospital of Brest.

Systematic overestimation of human serum albumin by capillary zone electrophoresis method due to monoclonal immunoglobulin interferences.

Background: The capillary zone electrophoresis method of albumin measurement is frequently used in monoclonal gammopathy patients but some studies suggest poor performances of the method in this population. The aim of this study was to analyse the impact of serum monoclonal immunoglobulins on human serum albumin determination by capillary zone electrophoresis method compared to other available methods.

Method: We prospectively measured albumin in 100 freshly collected non-frozen serum samples in a monoclonal gammopathy patients population, by using four different methods: the capillary zone electrophoresis method, the bromocresol purple dye method, the nephelometric method and the turbidimetric method.

Is capillary zone electrophoresis a suitable method for estimating serum albumin: A comparison of four methods.

Background: The capillary zone electrophoresis method of albumin measurement is frequently used for oncologic and haematologic patients but few data exist about the agreement between the albumin measurements performed by capillary zone electrophoresis and other methods. The aim of this study was to analyse the agreement between human serum albumin measurements by capillary zone electrophoresis and by the nephelometry, bromocresol purple and turbidimetry methods.

Method: We prospectively measured 100 freshly collected non-frozen patient serum samples, by using four different methods: the capillary zone electrophoresis method performed with a CAPILLARYS 2 instrument, the bromocresol purple dye method performed with an Advia XPT analyser, the nephelometric method performed with a BN ProSpec analyser and the turbidimetric method with reagents from DiAgam and performed with the Advia XPT analyser.

New innovative ecosystems in France to develop the Bioeconomy.

The cluster's policy initiated in France in 2005 was a formidable opportunity to boost innovation in the bioeconomy and to develop innovation platforms allowing industrial demonstration and feasibility at technology readiness level (TRL) from 5 to 9. The IAR Cluster (Industries and Agro-Resources), based in the Hauts-de-France and Grand Est regions is completely dedicated to the bioeconomy. IAR's work has contributed to build a complete innovation ecosystem on bioeconomy by opening up relationships between actors along the full value chain.

Early dynamic left intraventricular obstruction is associated with hypovolemia and high mortality in septic shock patients.

Introduction: Based on previously published case reports demonstrating dynamic left intraventricular obstruction (IVO) triggered by hypovolemia or catecholamines, this study aimed to establish: (1) IVO occurrence in septic shock patients; (2) correlation between the intraventricular gradient and volume status and fluid responsiveness; and (3) mortality rate.

Method: We prospectively analyzed patients with septic shock admitted to a general ICU over a 28-month period who presented Doppler signs of IVO. Clinical characteristics and hemodynamic parameters as well as echocardiographic data regarding left ventricular function, size, and calculated mass, and left ventricular outflow Doppler pattern and velocity before and after fluid infusions were recorded.

Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.

Toxoplasma gondii, a human pathogen and a model apicomplexan parasite, actively and rapidly invades host cells. To initiate invasion, the parasite induces the formation of a parasite-cell junction, and progressively propels itself through the junction, inside a newly formed vacuole that encloses the entering parasite. Little is known about how a parasite that is a few microns in diameter overcomes the host cell cortical actin barrier to achieve the remarkably rapid process of internalization (less than a few seconds).

[Evaluation of NT-proBNP as an early biological marker of cardiac dysfunction in septic shock].

Objective: To evaluate the NT-proBNP as a biological diagnosis marker of the myocardial dysfunction in septic shock.

Study Design: Non-randomized prospective clinical study with written assent. The analysis of the data obtained was retrospective.

Differential regulation of estrogen receptor alpha turnover and transactivation by Mdm2 and stress-inducing agents.

In mammalian cells, the level of estrogen receptor alpha (ERalpha) is rapidly decreased upon estrogen treatment, and this regulation involves proteasome degradation. Using different approaches, we showed that the Mdm2 oncogenic ubiquitin-ligase directly interacts with ERalpha in a ternary complex with p53 and is involved in the regulation of ERalpha turnover (both in the absence or presence of estrogens). Several lines of evidence indicated that this effect of Mdm2 required its ubiquitin-ligase activity and involved the ubiquitin/proteasome pathway.

A thermodynamic study of GPI-anchored and soluble form of alkaline phosphatase films at the air-water interface.

Glycosylphosphatidyl inositol (GPI) anchored proteins are localized and clustered on the outer layer of the plasma membranes forming microdomains. Among them, mammalian alkaline phosphatases (AP-GPI) are widely distributed enzymes. They can also exist as soluble proteins without anchor (APs).

Effect of the polar headgroup of phospholipids on their interaction with actin.

It is generally admitted that actin filaments are anchored to a membrane by membranar actin-binding-proteins. However, we found that actin may also interact directly with membrane phospholipids. The actin-phospholipid complex has been investigated at the air-water interface using a film balance technique.

Optimisation of a silicon/silicon dioxide substrate for a fluorescence DNA microarray.

This paper presents a comprehensive theory and experimental characterisation of the modulation of the fluorescence intensity by the construction of optical interferences on oxidised silicon substrates used for DNA microarrays. The model predicts a 90-fold variation of the fluorescence signal depending on the oxide thickness. For a Cy3 dye, the signal is maximal for a 90 nm oxide thickness corresponding to a 7.

Adsorption of a phospholipid-hydroperoxide glutathione peroxidase into phospholipid monolayers at the air-water interface.

The interfacial behavior differences of two glutathione peroxidase isoforms have been investigated. The first isoform is the phospholipid-hydroperoxide glutathione peroxidase (EC 1.11.

Streptomyces chromofuscus phospholipase D interaction with lipidic activators at the air-water interface.

The phospholipase D from Streptomyces chromofuscus (PLDSc) is a soluble enzyme that interacts with membranes to catalyse phosphatidylcholine (PC) transformation. In this work, we focused on the interaction between PLDSc and two lipid activators: a neutral lipid, diacylglycerol (DAG), and an anionic one, phosphatidic acid (PA). DAG is a naturally occurring alcohol, so it is a potent nucleophile for the transphosphatidylation reaction catalysed by PLD.

Specific interaction of the antiapoptotic protein Nr-13 with phospholipid monolayers is prevented by the BH3 domain of Bax.

Members of the Bcl-2 protein family regulate apoptosis by controlling the release of apoptogenic proteins such as cytochrome c from the mitochondrial intermembrane space. Proapoptotic members induce release by increasing outer membrane permeability, while antiapoptotic members prevent this. The activity of Bcl-2 proteins depends mostly on their insertion into the mitochondrial membrane, which is reported to occur via putative channels formed by the two central hydrophobic helices.

Preferential orientation of an immunoglobulin in a glycolipid monolayer controlled by the disintegration kinetics of proteo-lipidic vesicles spread at an air-buffer interface.

The insertion of immunoglobulin (IgG) in a glycolipid monolayer was achieved by using the ability of new proteo-glycolipid vesicles to disintegrate into a mixed IgG-glycolipid interfacial film after spreading at an air-buffer interface. The interfacial disintegration kinetics was shown to be directly dependent on the initial vesicle surface density and on the buffer ionic strength. The presence of the immunoglobulin in the glycolipid film was displayed by an increase of the lateral compressibility (Cs) during monolayer compression.