Spheroid Culture System, a Promising Method for Chondrogenic Differentiation of Dental Mesenchymal Stem Cells.

The objective of the present work was to develop a three-dimensional culture model to evaluate, in a short period of time, cartilage tissue engineering protocols. The spheroids were compared with the gold standard pellet culture. The dental mesenchymal stem cell lines were from pulp and periodontal ligament.

Temporomandibular disorders in children and adolescents: A review.

Background: Temporomandibular disorders (TMD) are generally observed in individuals between the ages of 20 and 40 years. TMD have also been described in children and adolescents but are still not widely detected and treated in routine practice. Through a literature review, this work aims to improve the diagnosis and management of TMD in children and adolescents by dentists.

Osteoarthritis of the Temporomandibular Joint: A Narrative Overview.

: This study reviewed the literature to summarize the current and recent knowledge of temporomandibular joint osteoarthritis (TMJOA). : Through a literature review, this work summarizes many concepts related to TMJOA. : Although many signaling pathways have been investigated, the etiopathogenesis of TMJOA remains unclear.

[Ehlers-Danlos syndrome: Role of the dentist].

The Ehlers-Danlos syndrome (EDS) is a group of genetic connective tissue disorder due to abnormal collagen synthesis. EDS is characterized by the triad: joint hypermobility - skin hyperextensibility - connective tissue friability leading to vascular and skin fragility. Thirteen forms exist including three main ones: the hypermobile, classical and vascular forms.

Relationship between natural or iatrogenic malocclusions and temporomandibular disorders: A case control study.

Objective: To investigate the link between malocclusions and temporomandibular disorders (TMD) with a focus on iatrogenic malocclusion. Other etiologies of TMD (oral parafunctions) were also assessed.

Methods: The prevalence of malocclusions was correlated in two groups: patients with TMD (case group) and patients without TMD (control group).

Enhancement of the biocompatibility by surface nitriding of a low-modulus titanium alloy for dental implant applications.

To enhance their longevity, dental implants must be highly biocompatible and must have a low elastic modulus close to that of the bone. They must also possess a high superficial hardness and a high corrosion resistance. For these reasons, a recently developed low-modulus Ti-27Nb alloy with nontoxic elements was treated by gas nitriding at high temperature in this study.

3D cell culture to determine in vitro biocompatibility of bioactive glass in association with chitosan.

This study reports the in vitro biocompatibility of a composite biomaterial composed of 46S6 bioactive glass in association with chitosan (CH) by using 3D osteoblast culture of SaOS2. The 46S6 and CH composite (46S6-CH) forms small hydroxyapatite crystals on its surface after only three days immersion in the simulated body fluid. For 2D osteoblast culture, a significant increase in cell proliferation was observed after three days of contact with 46S6 or 46S6-CH-immersed media.

[Relations between extraction of wisdom teeth and temporomandibular disorders: a case/control study].

The aim of this study was to assess the impact of extraction of third molars on the occurrence of temporo-mandibular disorders (TMD). A review of the literature and a case-control study have been conducted. The case-control study compares the frequency of extraction of third molars between the sample with TMD (case) and the sample without TMD (control).

[Relations between orthognathic surgery and temporomandibular disorders: a systematic review].

A painful and dysfunctional temporomandibular joint can be associated with a musculoskeletal anomaly. The multifactorial character of the etiology is now recognized. Among the etiologies, the role of orthognathic surgery is still debated.

Evaluation of the capacity of the SCGE assay to assess the genotoxicity of biomaterials.

The comet test or SCGE assay, which is already widely used in other areas, has never been used to evaluate the mutagenic potential of medical biomaterials in the final form. The purpose of our study was thus to assess the comet test as a means of assessing the genotoxic potential of finished medical biomaterials. We used silicone elastomers with increasing concentrations of 4-nitroquinoline oxide, a genotoxic agent.

Evaluation of the in vitro biocompatibility of various elastomers.

A previous study highlighted the superior shock absorption of silicone rubbers compared to other elastomers. We evaluated and compared the in vitro biocompatibility of silicone-based rubbers and propose them as an alternative to conventional products. We used the MTT colorimetric test to assess cell viability and flow cytometry to evaluate cell proliferation.