Generation of Pure Highly Functional Human Anti-Tumor Specific Cytotoxic T Lymphocytes With Stem Cell-Like Memory Features for Melanoma Immunotherapy.

Adoptive immunotherapy based on the transfer of anti-tumor cytotoxic T lymphocytes (CTLs) is a promising strategy to cure cancers. However, rapid expansion of numerous highly functional CTLs with long-lived features remains a challenge. Here, we constructed NIH/3T3 mouse fibroblast-based artificial antigen presenting cells (AAPCs) and precisely evaluated their ability to circumvent this difficulty.

Direct Toll-Like Receptor 8 signaling increases the functional avidity of human CD8+ T lymphocytes generated for adoptive T cell therapy strategies.

Adoptive transfer of in vitro activated and expanded antigen-specific cytotoxic T lymphocytes (CTLs) is a promising therapeutic strategy for infectious diseases and cancers. Obtaining in vitro a sufficient amount of highly specific cytotoxic cells and capable of retaining cytotoxic activity in vivo remains problematic. We studied the role of Toll-Like Receptor-8 (TLR8) engagement on peripheral CTLs activated with melanoma antigen MART-1-expressing artificial antigen-presenting cells (AAPCs).

Noise effect on comfort in open-space offices: development of an assessment questionnaire.

It is currently accepted that noise is one of the most important annoyance factors in open-space offices. However, noise levels measured in open spaces of the tertiary sector rarely exceed 65 dB(A). It, therefore, appears necessary to develop a tool that can be used to assess the noise environment of these offices and identify the parameters to be taken into consideration when assessing the noise annoyance.

Implementation of diffraction in a ray-tracing model for the prediction of noise in open-plan offices.

Sound prediction in open-plan offices is a real challenge because of the complexity of the layout of such offices, and therefore because of the multitude of acoustic phenomena involved. One such phenomenon, of primary importance, and not the least challenging of them, is the diffraction by screens and low dividers that usually partition the workspace. This paper describes implementing the equations of the Uniform Theory of Diffraction [McNamara et al.

Survey of noise exposure and background noise in call centers using headphones.

Call centers represent one of the fastest growing industries. However, there are health and safety hazards unique to this new industry. One of these potential hazards is hearing impairment caused by headsets.

Spatial undersampling for synthetic aperture ultrasonic imaging via digital spotlighting technique.

High resolution ultrasonic imaging may be achieved via the synthetic aperture focusing technique, provided that the synthetic antenna is correctly spatially sampled. We show that spatial undersampling is possible, using a digital spotlighting technique that permits to recover the correct reconstructed images from aliased data. The proposed method is applied to experimental ultrasonic data.

Representation and performance: reproduction of form and quality of movement in dance by eight- and 11-year-old novices.

This study examined the effects of age on the cognitive representations in modelling. A total of 48 eight- and 11-year-olds were asked to watch a dancer performing a ballet sequence of three steps in order to reproduce it. They were able to see the film as many times as they wanted.

[The behavior of the general practitioner in dealing with tachycardia].

In medical practice the physician may face 3 situations when dealing with tachycardia or arrhythmia: 1) when seen, the patient has tachycardia and the physician has no electrocardiograph: 2) the patient has tachycardia and the physician can take an electrocardiogramm; 3) the patient discloses a history suggestive of tachycardia or arrhythmia. In the first situation the heart rate and rhythm must be carefully observed immediately, as well as jugular venous pulse and heart sounds and murmurs; then the effects of respiration, change in posture, and carotid sinus pressure should be evaluated; finally, a complete physical examination should be carried out and the patient's history taken; for the latter, questions should be asked concerning past episodes, intake of digitalis or other drugs, possible reasons for hypokalemia, and presence of any disease that might play a role. In the second situation, the ECG should be recorded at once by the physician himself (first V1-2 and DII); the subsequent workup is the same as for the first situation.