Publications by authors named "Charlotte van Rooijen"
Article Synopsis
- Spondyloarthritis (SpA) involves abnormal bone growth and inflammation, and current treatments mainly address inflammation but not bone growth; this study focuses on how specific blood vessels (type H) might play a role in SpA pathology.
- Researchers studied tmTNF-Tg mice, which mimic SpA features, and found increases in type H vessels and bone-forming cells before clinical symptoms appeared, indicating early changes in the disease.
- The findings suggest that type H vessels contribute to pathologic bone growth in SpA and highlight potential new treatment strategies targeting these vessels in the disease's progression.
Endothelial cells (ECs) are important contributors to inflammation in immune-mediated inflammatory diseases (IMIDs). In this study, we examined whether CD4 memory T (T) cells can drive EC inflammatory responses. Human T cells produced ligands that induced inflammatory responses in human umbilical vein EC as exemplified by increased expression of inflammatory mediators including chemokines and adhesion molecules.
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- The study focused on ductal carcinoma in situ (DCIS) and the need to differentiate harmless lesions from those that could become invasive breast cancer.
- Using a Dutch cohort of over 10,000 women with DCIS, researchers analyzed the immune microenvironment and its characteristics in relation to subsequent invasive breast cancer.
- Results showed that while certain immune cell densities correlated with cancer characteristics, they did not predict the transition to invasive cancer in the observed patients.
Article Synopsis
- Soluble tumor necrosis factor (sTNF) is crucial for the development and function of secondary lymphoid organs (SLO), whereas the role of its transmembrane form (tmTNF) was less clear until this study.
- Researchers used tmTNF-transgenic (tmTNF-tg) mice and found that tmTNF overexpression led to enlarged lymph nodes and spleens, but with disorganized B and T cell areas and lower germinal center B cells.
- The overexpression also negatively impacted the formation of plasma cells and shifted their antibody production, with normalization of some features observed upon genetic deletion of TNFRI or -II, indicating that tmTNF is key for SLO development and function
TNF is important in immune-mediated inflammatory diseases, including spondyloarthritis (SpA). Transgenic (tg) mice overexpressing transmembrane TNF (tmTNF) develop features resembling human SpA. Furthermore, both tmTNF tg mice and SpA patients develop ectopic lymphoid aggregates, but it is unclear whether these contribute to pathology.
View Article and Find Full Text PDFPreoperative immunotherapy with anti-PD1 plus anti-CTLA4 antibodies has shown remarkable pathological responses in melanoma and colorectal cancer. In NABUCCO (ClinicalTrials.gov: NCT03387761 ), a single-arm feasibility trial, 24 patients with stage III urothelial cancer (UC) received two doses of ipilimumab and two doses of nivolumab, followed by resection.
View Article and Find Full Text PDFDuring processing of pet food, the Maillard reaction occurs, which reduces the bioavailability of essential amino acids such as lysine and results in the formation of advanced Maillard reaction products (MRPs). The aim of this study was to quantitate MRPs (fructoselysine (FL), carboxymethyllysine (CML), hydroxymethylfurfural (HMF)) and the cross-link lysinoalanine (LAL) in commercial pet foods. Sixty-seven extruded, canned, and pelleted dog and cat foods for growth and maintenance were analyzed using UPLC-MS.
View Article and Find Full Text PDFThe Maillard reaction can occur during processing of pet foods. During this reaction, the ε-amino group of lysine reacts with reducing sugars to become unavailable for metabolism. The aim of the present study was to determine the reactive lysine (RL; the remaining available lysine) to total lysine (TL) ratio of commercial pet foods and to evaluate whether RL levels meet minimal lysine requirements (MLR).
View Article and Find Full Text PDFThe Maillard reaction, which can occur during heat processing of pet foods or ingredients, is known to reduce the bioavailability of essential amino acids such as lysine due to the formation of early and advanced Maillard reaction products (MRP) that are unavailable for utilisation by the body. Determination of the difference between total and reactive lysine by chemical methods provides an indication of the amount of early MRP present in foods, feeds and ingredients. Previous research reported that the difference between total and reactive lysine in pet foods can be up to 61.
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