Digital PCR-Based Gene Expression Analysis Using a Highly Multiplexed Assay with Universal Detection Probes to Study Induced Pluripotent Stem Cell Differentiation into Cranial Neural Crest Cells.

Induced pluripotent stem cells (iPSCs) have the potential to differentiate into any cell type, offering a valuable tool for research in developmental biology, regenerative medicine, and disease modeling. In this study, iPSCs were differentiated into cranial neural crest cells (CNCCs) over a 14-day period. RNA was extracted from these cells at day 0 (iPSCs), day 7, and day 14 to evaluate successful differentiation through the expression analysis of pluripotency and CNCC marker genes.