Publications by authors named "Charlotte Roux"
Article Synopsis
- RNA polyadenylation, led by poly(A) polymerases, uses ATP to add adenine to RNA, which can impact the stability of those RNA molecules.
- This study explored what happens when the gene for the main poly(A) polymerase, PAP I, is inactivated, finding that over a thousand RNAs were stabilized and ATP levels dropped by 20%.
- The research revealed a direct connection between ATP levels and RNA stability, indicating that low ATP can stabilize RNAs when PAP I activity is reduced, highlighting the role of energy in RNA metabolism.
Article Synopsis
- - Small nucleolar RNAs (snoRNAs) are non-coding RNAs crucial for the creation of ribosomes and spliceosomes by targeting specific sites on ribosomal and spliceosomal RNAs for modifications, though many snoRNAs still lack comprehensively identified functions.
- - An enhanced method called chimeric eCLIP was developed to investigate snoRNA-target RNA interactions, confirming known interactions in cell lines and discovering new ones, particularly for orphan snoRNAs.
- - The study found that snoRNA complexes with certain accessory proteins, such as WDR43 and NOLC1, have unique interactions that influence ribosome and spliceosome biogenesis, highlighting the dual role of snoRNAs in modifying RNA and regulating splicing processes.
RNA splicing is pivotal in post-transcriptional gene regulation, yet the exponential expansion of intron length in humans poses a challenge for accurate splicing. Here, we identify hnRNPM as an essential RNA-binding protein that suppresses cryptic splicing through binding to deep introns, maintaining human transcriptome integrity. Long interspersed nuclear elements (LINEs) in introns harbor numerous pseudo splice sites.
View Article and Find Full Text PDFMetabolic engineering strategies are crucial for the development of bacterial cell factories with improved performance. Until now, optimal metabolic networks have been designed based on systems biology approaches integrating large-scale data on the steady-state concentrations of mRNA, protein and metabolites, sometimes with dynamic data on fluxes, but rarely with any information on mRNA degradation. In this review, we compile growing evidence that mRNA degradation is a key regulatory level in E.
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