A comparison of internal and external lipids of nondiapausing and diapause initiation phase adult Colorado potato beetles, Leptinotarsa decemlineata.

The Colorado potato beetle, Leptinotarsa decemlineata, reared under diapause-inducing conditions will emerge from the soil as an adult and enter the diapause initiation phase, a period where metabolic reserves are stockpiled before the beetles enter the nonfeeding diapause maintenance phase. Internal and external lipids were characterized during the diapause initiation phase (IP) and compared to the lipid profiles of nondiapausing adults. The primary internal lipids of both diapause IP and nondiapausing adults are triacylglycerols.

Cuticular lipids of female solitary bees, Osmia lignaria Say and Megachile rotundata (F.) (Hymenoptera: Megachilidae).

The cuticular lipids of the cavity-nesting adult female solitary bees, Osmia lignaria Say and Megachile rotundata (F.) (Hymenoptera: Megachilidae), were analyzed by gas chromatography (GC) and combined GC-mass spectrometry. The cuticular lipids of these female bees are mainly consisted of hydrocarbons.

Hydrocarbons in the surface wax of eggs and adults of the Colorado potato beetle, Leptinotarsa decemlineata.

The major components of the egg hydrocarbons were dimethylalkanes (40%) and trimethylalkanes (24%) in which the first methyl branch was on carbon 2. The major dimethylalkanes were an approximately 2:1 mixture of 2,10- and 2,6-dimethyloctacosanes in females and eggs. The major trimethylalkanes were a mixture of 2,10,16- and 2,10,18-trimethyloctacosanes.

Cuticular hydrocarbons of the flea beetles, Aphthona lacertosa and Aphthona nigriscutis, biocontrol agents for leafy spurge (Euphorbia esula).

The adult beetles Aphthona lacertosa and Aphthona nigriscutis, used as biocontrol agents for leafy spurge, had a complex mixture of hydrocarbons on their cuticular surface consisting of alkanes, methylalkanes, alkenes and alkadienes as determined by gas chromatography-mass spectrometry. A trace amount of wax esters were present. In both species, the hydrocarbons were the major cuticular lipid class and the gas chromatographic profiles of the total hydrocarbons were similar.