Publications by authors named "Charlotte Demkes"
Article Synopsis
- The study investigates the molecular mechanisms behind pathological cardiac remodeling, focusing on cardiomyocyte (CM) hypertrophy that can lead to heart failure (HF).
- Researchers utilized a mouse model to analyze gene expression profiles during different stages of hypertrophy, revealing specific genes up-regulated during maladaptive hypertrophy, including a previously uncharacterized gene, Pfkp.
- The findings suggest that Pfkp plays a crucial role in the maladaptive remodeling of CMs, providing insights that could help develop targeted therapies for heart failure.
Rationale: CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated protein 9)-based DNA editing has rapidly evolved as an attractive tool to modify the genome. Although CRISPR/Cas9 has been extensively used to manipulate the germline in zygotes, its application in postnatal gene editing remains incompletely characterized.
Objective: To evaluate the feasibility of CRISPR/Cas9-based cardiac genome editing in vivo in postnatal mice.
MicroRNAs (miRNAs) are important regulators of biology and disease. Recent animal efficacy studies validate the therapeutic benefit of miRNA modulation and underscore the therapeutic value of miRNA-targeting oligonucleotides. However, whether disease conditions (stress) influence the pharmacological effects of an anti-miR is currently unknown.
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