Publications by authors named "Charles M Bulathsinghala"
Article Synopsis
- The Escherichia coli TonB system includes membrane proteins TonB, ExbB, ExbD, and various transporters for iron and vitamin B12, which facilitate the transmission of proton motive force (PMF) from the cytoplasmic to the outer membrane.
- Characterization of ExbB's cytoplasmic loop through 10-residue deletions (Δ10 proteins) showed that these deletions hinder all TonB-dependent functions, including the interaction between TonB and ExbD, and induce a reversible growth arrest that is independent of PMF collapse.
- The growth arrest caused by Δ10 proteins is linked to altered interactions with unknown growth-regulatory proteins, indicating that the ExbB cytoplasmic loop might
The active transport of iron siderophores and vitamin B(12) across the outer membrane (OM) of Escherichia coli requires OM transporters and the potential energy of the cytoplasmic membrane (CM) proton gradient and CM proteins TonB, ExbB, and ExbD. A region at the amino terminus of the transporter, called the TonB box, directly interacts with TonB Q160 region residues. R158 and R166 in the TonB Q160 region were proposed to play important roles in cocrystal structures of the TonB carboxy terminus with OM transporters BtuB and FhuA.
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