A FA1-targeting albumin marker enables the ratiometric detection of apixaban in urine.

A FA1-targeting albumin marker along with apixaban can form a co-binding complex with albumin, resulting in a readily discernible fluorescence color change.

Is thymectomy necessary during parathyroidectomy for secondary hyperparathyroidism in patients with end-stage kidney disease?

Background: Guidelines recommend thymectomy at the time of parathyroidectomy for secondary hyperparathyroidism to reduce the likelihood of persistent or recurrent disease. We sought to determine the frequency of thymectomy and explore its impact on recurrence of secondary hyperparathyroidism.

Methods: Using TriNetX, a multi-institutional electronic health record and insurance claims network, we conducted a retrospective cohort study of adults with secondary hyperparathyroidism who underwent parathyroidectomy with or without thymectomy from 2005 to 2023.

Albumin host for supramolecular fluorescence recognition.

Synthetic molecular sensors are crucial for real-time monitoring in biological systems and biotechnological applications, where detecting targets amidst potential interferents is essential. This task is particularly challenging in competitive environments that lacking chemically reactive functional groups, common in agricultural, biological, and environmental contexts. Consequently, scientific efforts have focused on developing sensitive and rapid analytical techniques, with fluorescent sensors emerging as prominent tools.

Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution.

The molecular organization of cells and tissue is challenging to study due to the inefficiency of multiplexed molecular labeling methods and the limited options for combining microscopy modalities in a single specimen, especially when high spatial resolution is needed. Here we describe ultraplex microscopy, which combines serial multiplexing, ultrathin sectioning, and reversible embedding to circumvent incompatibilities between labeling and imaging techniques, enhance resolution, and expand multiplexing capacity within and across modalities. Samples can be labeled with antibodies, RNA probes, and tissue stains for imaging by brightfield, epifluorescence, super-resolution, and electron microscopy without specialized reagents or materials.

A smartphone-based supramolecular biosensor for portable and rapid detection of buprofezin in real food samples.

Buprofezin (BUP) is an insect growth regulator widely used in agriculture to control hemipteran pests, particularly the melon aphid, Aphis gossypii, due to its efficiency and low toxicity. Although approved by the Chinese government, its maximum residue limit (MRL) in food is strictly regulated, and conventional techniques for detecting BUP have several limitations. Our study reports successful BUP detection using a supramolecular fluorescent probe DP@ALB, constructed with chalcone-based fluorescent dye DP and albumin as the host.

Boosting smartphone-assisted on-site monitoring capacity for nitroxynil using synergistic fluorescent sensing system.

A novel ratiometric analytical method based on a quantum dot (QD)-integrated supramolecular sensing system successfully achieves the portable on-site detection of nitroxynil.

Insights From a Clinically Orientated Workshop on Health Care Cybersecurity and Medical Technology: Observational Study and Thematic Analysis.

Background: Health care professionals receive little training on the digital technologies that their patients rely on. Consequently, practitioners may face significant barriers when providing care to patients experiencing digitally mediated harms (eg, medical device failures and cybersecurity exploits). Here, we explore the impact of technological failures in clinical terms.

"People are now working together for a common good": The effect on social capital of participatory design for community-level sanitation infrastructure in urban informal settlements.

Communities with higher levels of social capital perform better than communities with lower social capital in community-level water and sanitation interventions and have better health outcomes. Although research recommends bolstering social capital to improve intervention outcomes, few studies provide empirical evidence on the effect of intervention activities on social capital. This study aimed to evaluate the effect of participatory design and community engagement activities on social capital among urban informal settlements in Suva, Fiji and Makassar, Indonesia enrolled in the Revitalizing Informal Settlements and their Environments trial using the Short Adapted Social Capital Assessment Tool.

Influence of triazines and lipopolysaccharide coexposure on inflammatory response and histopathological changes in the testis and liver of BalB/c mice.

Background: Triazines are environmental active chemicals that have been reported to alter the inflammatory status of the gonads. We tested the anti-inflammatory effect of the triazines (atrazine; ATZ, simazine; SMZ and cyanazine; CYZ) on the testis and compared it with the more classical liver model that has substantial populations of resident macrophages comparable to the testis.

Methods: BalB/c mice were treated with 25 mg/kg ATZ, SMZ and CYZ for 30 days and injected with lipopolysaccharide (0.

Shoe configuration effects on third phalanx and capsule motion of unaffected and laminitic equine hooves in-situ.

Equine shoes provide hoof protection and support weakened or damaged hoof tissues. Two hypotheses were tested in this study: 1) motion of the third phalanx (P3) and hoof wall deformation are greater in laminitic versus unaffected hooves regardless of shoe type; 2) P3 displacement and hoof wall deformation are greatest while unshod (US), less with open-heel (OH), then egg-bar (EB) shoes, and least with heart-bar (HB) shoes for both hoof conditions. Distal forelimbs (8/condition) were subjected to compressive forces (1.

Joint Food and Water Insecurity Had a Multiplicative Effect on Women's Depression in Urban Informal Settlements in Makassar, Indonesia during the COVID-19 Pandemic.

Background: Women living in urban informal settlements may be particularly vulnerable to the detrimental effects of the COVID-19 pandemic because of increased economic and psychosocial stressors in resource-limited environments.

Objectives: The objective of this study was to assess the associations between food and water insecurity during the pandemic and depression among women living in the urban informal settlements in Makassar, Indonesia.

Methods: We implemented surveys at 3 time points among women enrolled in the Revitalizing Informal Settlements and their Environments trial.

Genome-wide analysis of genes involved in efflux function and regulation within and serovar Typhimurium.

The incidence of multidrug-resistant bacteria is increasing globally, with efflux pumps being a fundamental platform limiting drug access and synergizing with other mechanisms of resistance. Increased expression of efflux pumps is a key feature of most cells that are resistant to multiple antibiotics. Whilst expression of efflux genes can confer benefits, production of complex efflux systems is energetically costly and the expression of efflux is highly regulated, with cells balancing benefits against costs.

Genome-Wide Analysis of Innate Susceptibility Mechanisms of to Colistin.

Colistin is an antibiotic that has seen increasing clinical use for the treatment of human infections caused by Gram-negative pathogens, particularly due to the emergence of multidrug-resistant pathogens. Colistin resistance is also a growing problem and typically results from alterations to lipopolysaccharides mediated by phosphoethanolamine (pETn) transferase enzymes which can be encoded on the chromosome, or plasmids. In this study, we used 'TraDIS-Xpress' (nsposon irected nsertion site equencing with eion), where a high-density transposon mutant library including outward facing promoters in BW25113 identified genes involved in colistin susceptibility.

Comparison of the genetic basis of biofilm formation between Typhimurium and .

Most bacteria can form biofilms, which typically have a life cycle from cells initially attaching to a surface before aggregation and growth produces biomass and an extracellular matrix before finally cells disperse. To maximize fitness at each stage of this life cycle and given the different events taking place within a biofilm, temporal regulation of gene expression is essential. We recently described the genes required for optimal fitness over time during biofilm formation in using a massively parallel transposon mutagenesis approach called TraDIS-.

QT interval and ventricular action potential prolongation in the Mecp2 murine model of Rett syndrome.

Rett Syndrome (RTT) is a congenital, X-chromosome-linked developmental disorder characterized by developmental delay, dysautonomia, and breathing irregularities. RTT is also associated with sudden death and QT intervals are prolonged in some RTT patients. Most individuals with RTT have mutations in the MECP2 gene.

A whole-genome assay identifies four principal gene functions that confer tolerance of meropenem stress upon .

We report here the identification of four gene functions of principal importance for the tolerance of meropenem stress in : cell division, cell envelope synthesis and maintenance, ATP metabolism, and transcription regulation. The primary mechanism of β-lactam antibiotics such as meropenem is inhibition of penicillin binding proteins, thus interfering with peptidoglycan crosslinking, weakening the cell envelope, and promoting cell lysis. However, recent systems biology approaches have revealed numerous downstream effects that are triggered by cell envelope damage and involve diverse cell processes.

An efficient 2-aminothiazolesalicylaldehyde fluorescent chemosensor for Fe ion detection and a potential inhibitor of NUDT5 signaling hormone for breast cancer cell and molecular keypad lock application.

Unlabelled: A novel thiazole phenol conjugate, 2-aminothiazolesalicylaldehyde (receptor1) was designed and synthesized for the first time through a single step process via Schiff base condensation reaction. The formation of receptor1 was confirmed by FTIR, C NMR, and H NMR. The IR spectra confirmed the presence of the aldimine formation.

Workshop-based learning and networking: a scalable model for research capacity strengthening in low- and middle-income countries.

Science education and research have the potential to drive profound change in low- and middle-income countries (LMICs) through encouraging innovation, attracting industry, and creating job opportunities. However, in LMICs, research capacity is often limited, and acquisition of funding and access to state-of-the-art technologies is challenging. The Alliance for Global Health and Science (the Alliance) was founded as a partnership between the University of California, Berkeley (USA) and Makerere University (Uganda), with the goal of strengthening Makerere University's capacity for bioscience research.

Genomic Analysis of Carbapenem-Resistant in Water Matrices: Implications for Public Health and Wastewater Treatments.

Comamonas spp. are Gram-negative bacteria that catabolize a wide range of organic and inorganic substrates. Comamonas spp.

Delayed Ventricular Repolarization and Sodium Channel Current Modification in a Mouse Model of Rett Syndrome.

Rett syndrome (RTT) is a severe developmental disorder that is strongly linked to mutations in the gene. RTT has been associated with sudden unexplained death and ECG QT interval prolongation. There are mixed reports regarding QT prolongation in mouse models of RTT, with some evidence that loss of function enhances cardiac late Na current, I.

Long-read sequencing for identification of insertion sites in large transposon mutant libraries.

Transposon insertion site sequencing (TIS) is a powerful method for associating genotype to phenotype. However, all TIS methods described to date use short nucleotide sequence reads which cannot uniquely determine the locations of transposon insertions within repeating genomic sequences where the repeat units are longer than the sequence read length. To overcome this limitation, we have developed a TIS method using Oxford Nanopore sequencing technology that generates and uses long nucleotide sequence reads; we have called this method LoRTIS (Long-Read Transposon Insertion-site Sequencing).

Design and characteristic analysis of an all-optical AND, XOR, and XNOR Y-shaped MIM waveguide for high-speed information processing.

All-optical logic gates are exceptionally suited for Boolean ultrahigh-speed operation and logical computing. This study presents a plasmonic model that uses a Y-shaped metal-insulator-metal waveguide structure that realizes the ultrafast all-optical AND, XOR, and XNOR gate operation that is developed at a footprint of 6.6µ×3.

Genomic analysis of species from aquatic environments: Evidence for potential clinical transmission.

species are ubiquitous in aquatic environments, colonize water systems in healthcare settings and are emerging opportunistic pathogens with reports surfacing in 25 countries across six continents infections are challenging to treat, and case fatality rates are high. Chromosomal and genes encoding carbapenemases and cephalosporinases are unique to . and reports of concomitant resistance to aminoglycosides, fluoroquinolones and sulfamethoxazole-trimethoprim are known.