Exploring salivary lipid profile changes in COVID-19 patients: Insights from mass spectrometry analysis.

The most common COVID-19 testing relies on the use of nasopharyngeal swabs. However, this sampling step is very uncomfortable and is one of the biggest challenges regarding population testing. In the present study, the use of saliva as an alternative sample for COVID-19 diagnosis was investigated.

Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging.

Oral squamous cell carcinoma (OSCC) is the prevalent type of oral cavity cancer, requiring precise, accurate, and affordable diagnosis to identify the disease in early stages, Comprehending the differences in lipid profiles between healthy and cancerous tissues encompasses great relevance in identifying biomarker candidates and enhancing the odds of successful cancer treatment. Therefore, the present study evaluates the analytical performance of simultaneous mRNA and lipid extraction in gingiva tissue from healthy patients and patients diagnosed with OSCC preserved in TRIzol reagent. The data was analyzed by partial least-squares discriminant analysis (PLS-DA) and confirmed via matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI).

Determination of Pesticides in Soybean Seeds Incorrectly Discarded Near a Spring of the Paranaíba River, GO-Brazil.

The objective of this research was to evaluate the contamination of pesticides of carbamate and benzimidazole classes in soil, water and soybean seeds, incorrectly discarded near a spring in the state of Goiás-Brazil. The Solid-Liquid and Liquid-Liquid Extraction with Low Temperature Partition (SLE/LTP or LLE/LTP) methods were used for pesticide extraction and the analyses were performed by HPLC-UV. A high resolution mass spectrometer was used to confirm the identity of the compounds present in the seeds.