Combination of Machine Learning and Empirical Computation for the Structural Validation of Trirosaline, a Natural Trimeric Monoterpene Indole Alkaloid from .

Chemical investigation of the emblematic led to the discovery of trirosaline (), the first example of a tris-ajmalicine-type monoterpene indole alkaloid and the first natural trimeric MIA ever reported from this deeply dug plant species. Its structure was primarily elucidated based on NMR and HRESIMS analyses, and the nature of its unique intermonomeric linkages was firmly confirmed based on a combination of empirical computation and ML--DP4 study. Its absolute configuration was mitigated by comparison of experimental and TDDFT-simulated electronic circular dichroism (ECD) spectra.

Polybrominated diphenyl ethers isolated from the marine sponge collected in Mayotte.

CDK7 and FynB protein kinases have been recognized as relevant targets for cancer and brain diseases treatment due to their pivotal regulatory roles in cellular functions such as cell cycle and neural signal transduction. Several studies demonstrated that the inhibition of these proteins could be useful in altering the onset or progression of these diseases. Based on bioassay-guided approach, the extract of the marine sponge (Thorectidae), which exhibited interesting kinase inhibitory activities, was fractionated.

Screening an In-House Isoquinoline Alkaloids Library for New Blockers of Voltage-Gated Na Channels Using Voltage Sensor Fluorescent Probes: Hits and Biases.

Voltage-gated Na (Na) channels are significant therapeutic targets for the treatment of cardiac and neurological disorders, thus promoting the search for novel Na channel ligands. With the objective of discovering new blockers of Na channel ligands, we screened an In-House vegetal alkaloid library using fluorescence cell-based assays. We screened 62 isoquinoline alkaloids (IA) for their ability to decrease the FRET signal of voltage sensor probes (VSP), which were induced by the activation of Na channels with batrachotoxin (BTX) in GH3b6 cells.

New Metabolites from the Marine Sponge Collected in Mayotte Lagoon.

The biological screening of 44 marine sponge extracts for the research of bioactive molecules, with potential application in the treatment of age-related diseases (cancer and Alzheimer's disease) and skin aging, resulted in the selection of extract for chemical study. As no reports of secondary metabolites of were found in the literature, we undertook this research to further extend current knowledge of chemistry. The investigation of this species led to the discovery of four new compounds: two butenolides sinularone J () and sinularone K (), one phospholipid 1--octadecyl-2-pentanoyl--glycero-3-phosphocholine () and one lysophospholipid 1--(3-methoxy-tetradecanoyl)--glycero-3-phosphocholine () alongside with known lysophospholipids ( and ), alkylglycerols (-), epidioxysterols ( and ) and diketopiperazines ( and ).

Microorganisms Associated with the Marine Sponge : A Reservoir of Bioactive Molecules to Slow Down the Aging Process.

Aging research aims at developing therapies that delay normal aging processes and some related pathologies. Recently, many compounds and extracts from natural products have been shown to slow aging and/or extend lifespan. Marine sponges and their associated microorganisms have been found to produce a wide variety of bioactive secondary metabolites; however, those from the Southwest of the Indian Ocean are much less studied, especially regarding anti-aging activities.