Publications by authors named "Chara Sarafoglou"
The cytochrome P450 enzymes of the CYP4G subfamily are some of the most intriguing insect P450s in terms of structure and function. In Drosophila, CYP4G1 is highly expressed in the oenocytes and is the last enzyme in the biosynthesis of cuticular hydrocarbons, while CYP4G15 is expressed in the brain and is of unknown function. Both proteins have a CYP4G-specific and characteristic amino acid sequence insertion corresponding to a loop between the G and H helices whose function is unclear.
View Article and Find Full Text PDFGeranylgeranyl pyrophosphate (diphosphate) synthase (GGPPS) plays an important role in various physiological processes in insects, such as isoprenoid biosynthesis and protein prenylation. Here, we functionally characterised the GGPPS from the major agricultural lepidopteran pests Spodoptera frugiperda and Helicoverpa armigera. Partial disruption of GGPPS by CRISPR in S.
View Article and Find Full Text PDFArticle Synopsis
- - Contact insecticides like deltamethrin are used to control malaria-carrying Anopheles mosquitoes, with ABCH2 protein in their legs playing a significant role in insecticide resistance by affecting how well the insecticide penetrates.
- - Silencing the ABCH2 gene in mosquitoes increased their mortality from deltamethrin, indicating that ABCH2 helps regulate the insecticide's penetration rather than impacting the transport of hydrocarbons in their legs.
- - Research identified that ABCH2 functions as a half-transporter that can pump deltamethrin out of the mosquito, involving ATP hydrolysis, which supports the idea that this protein is a target for deltamethrin and affects toxicity regulation.