Generation and characterization of two acid-resistant macrocin -methyltransferase variants with a higher enzyme activity at 30 °C from .

Tylosin is an important macrolide antibiotic produced by . In the biosynthesis of tylosin, macrocin -methyltransferase TylF catalyzes the conversion of the side-product tylosin C (macrocin) to the primary component tylosin A (C/A conversion). This conversion is the rate-limiting step in the biosynthesis of tylosin, and affects the quality of the end product.

Resveratrol Alleviates Zearalenone-Induced Intestinal Dysfunction in Mice through the NF-κB/Nrf2/HO-1 Signalling Pathway.

Zearalenone (ZEA), a mycotoxin widely present in crops and food, poses a major threat to animal and human health. The consumption of ZEA-contaminated food or feed causes intestinal damage. Therefore, exploring how to mitigate the intestinal damage caused by its ZEA is becoming increasingly important.

Differential roles of and in trade-off between plant growth and drought tolerance.

Due to global climate change, drought is emerging as a major threat to plant growth and agricultural productivity. Abscisic acid (ABA) has been implicated in plant drought tolerance, however, its retarding effects on plant growth cannot be ignored. The reactions catalyzed by 1-deoxy-D-xylulose-5-phosphate synthase (DXS) and 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) proteins are critical steps within the isoprenoid biosynthesis in plants.

Discovery of a highly efficient TylF methyltransferase via random mutagenesis for improving tylosin production.

Macrolides are currently a class of extensively used antibiotics in human and animal medicine. Tylosin is not only one of the most important veterinary macrolides but also an indispensable material for the bio- and chemo-synthesis of new generations of macrolide antibiotics. Thus, improving its production yield is of great value.

A High-Throughput Method Based on Microculture Technology for Screening of High-Yield Strains of Tylosin-Producing .

Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of ; however, it is necessary to modify strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis.

The Metabolic Adaptation in Response to Nitrate Is Critical for Actinobacillus pleuropneumoniae Growth and Pathogenicity under the Regulation of NarQ/P.

Nitrate metabolism is an adaptation mechanism used by many bacteria for survival in anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood.

Cationic coordination polymers with thirteen-fold interpenetrating dia networks: selective coloration and ion-controlled photochromism.

A novel Cd-based cationic coordination polymer (Cd-CCP) constructed using viologen derivatives, which exhibits an unusual thirteen-fold interpenetrating diamondoid network, has been synthesized. Notably, Cd-CCP displays selective and naked-eye distinguished coloration and ion-controlled photochromism towards halide anions.

Viologen-Based Cationic Metal-Organic Framework for Efficient CrO Adsorption and Dye Separation.

A novel cationic metal-organic framework composed of {Cu(COO)} paddle-wheel units and a tetracarboxylic viologen derivative, namely, {[Cu(bdcbp)(HO)]·2NO·2HO} (, HbdcbpCl = 1,1'-bis(3,5-dicarboxyphenyl)-4,4'-bipyridinium dichloride), has been successfully synthesized and structurally characterized. In , the {Cu(COO)} unit and viologen derivative both act as four-connected nodes forming an ssb-type cationic network with 4.8 topology, in which the positive charges are distributed on the organic viologen moieties.