Is a Key Transcription Factor Controlling Sporulation, Hydrophobicity, and Stress Tolerance in .

has become one of the main pathogens in citrus due to its high spore production and easy spread. In this study, the function of the APSES transcription factor in was characterized, and the results indicated that it was involved in conidium and conidiophore development. No conidiophores were observed in the mycelium of the ∆ mutant that had grown for two days, while an abnormal conidiophore was found after another two days of incubation, and only small thin phialides as well as a very small number of spores were formed at the top of the hyphae.

The GRAS Salts of NaSiO and EDTA-Na Control Citrus Postharvest Pathogens by Disrupting the Cell Membrane.

Sodium silicate (NaSiO) and ethylenediaminetetraacetic acid disodium salt (EDTA-Na) are inorganic salts classified as 'Generally Recognized as Safe' (GRAS) compounds with great advantages in controlling various pathogens of postharvest fruits and vegetables. Here, we determined the median effective concentration (EC) of NaSiO (0.06%, 0.

GAR-transferase contributes to purine synthesis and mitochondrion function to maintain fungal development and full virulence of Penicillium digitatum.

Penicillium digitatum is one of the most critical phytopathogens during the citrus postharvest period. However, the molecular mechanism of pathogenesis remains to be further explored. Purine is a multiple functional substance in organisms.

Ferric Chloride Controls Citrus Anthracnose by Inducing the Autophagy Activity of .

causes citrus anthracnose, which seriously endangers the pre-harvest production and post-harvest storage of citrus due to its devastating effects on fruit quality, shelf life, and profits. However, although some chemical agents have been proven to effectively control this plant disease, little to no efforts have been made to identify effective and safe anti-anthracnose alternatives. Therefore, this study assessed and verified the inhibitory effect of ferric chloride (FeCl) against .

Biocontrol Ability and Action Mechanism of 37 on Soft Rot Control of Postharvest Kiwifruit.

Postharvest soft rot of kiwifruit has resulted in substantial market losses, yet there were few antagonistic yeasts reported to control the disease. This study screened 1113 yeast strains for potential antagonistic yeast to control soft rot of kiwifruit caused by Botryosphaeria dothidea and Diaporthe actinidiae, and strain 37 was selected to evaluate the control efficacy and mechanisms, which was identified as Meyerozyma guilliermondii via molecular biological identification. Our results showed that M.

Functional identification of the DHN melanin synthesis gene cluster and its role in UV-C tolerance in citrus postharvest pathogenic fungus Penicillium digitatum.

The filamentous fungus Penicillium digitatum brings out great losses in citrus fruits by causing citrus green mold disease during the postharvest period. Previously, we obtained a T-DNA insertion mutant N2130 of P. digitatum, which produced albino conidia.

Dimethyl Dicarbonate as a Food Additive Effectively Inhibits of Citrus.

Dimethyl dicarbonate (DMDC), a food additive, can be added to a variety of foods as a preservative. This study aimed to evaluate the inhibitory effects of DMDC on in vitro and in vivo, as well as the potential antifungal mechanism. In vitro experiments showed that 250 mg/L DMDC completely inhibited the growth of and significantly inhibited spore germination by 96.

Natamycin as a safe food additive to control postharvest green mould and sour rot in citrus.

Aims: The purpose of this study was to explore the potential inhibitory mechanism and assess the feasibility of natamycin as an antifungal agent in the utilization of citrus storage.

Methods And Results: In this study, the mycelial growth, spore germination as well as germ tube elongations of Geotrichum citri-aurantii and Penicillium digitatum were significantly inhibited by natamycin treatment. The relative conductivities of G.

Chromosome Genome Sequencing and Comparative Transcriptome-Based Analyses of 34-9 Unveil the Potential Biocontrol Mechanisms Against Citrus Green Mold.

Biological control is an environmentally friendly, safe, and replaceable strategy for disease management. Genome sequences of a certain biocontrol agent could lay a solid foundation for the research of molecular biology, and the more refined the reference genome, the more information it provides. In the present study, a higher resolution genome of 34-9 was assembled using high-throughput chromosome conformation capture (Hi-C) technology.

Genome sequencing and transcriptome analysis of Geotrichum citri-aurantii on citrus reveal the potential pathogenic- and guazatine-resistance related genes.

Sour rot, caused by Geotrichum citri-aurantii, is a major postharvest disease of citrus，and it causes serious economic losses. In this study, a high-quality genome sequence of G. citri-aurantii was obtained by Single Molecule Real-Time Sequencing (SMRT).

Genome-wide identification, characterization and expression analysis of lineage-specific genes within Hanseniaspora yeasts.

Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp.

The Shared and Specific Genes and a Comparative Genomics Analysis within Three Strains.

plays an important role in the inhibition of citrus postharvest blue and green mould diseases. This study was based on the previous genome sequencing of strain 34-9. After homologous comparison, scaffold 27 was defined as the mitochondrial (mt) sequence of 34-9.

Development of -Specific Primers for Rapid Detection among Fungal Isolates in Citrus.

Blue mold in citrus is caused by . In this study, the -specific primers were developed for rapid detection based on the conserved genes and among genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detect .

Antagonistic Activity and the Mechanism of Bacillus amyloliquefaciens DH-4 Against Citrus Green Mold.

Citrus fruit usually suffer significant losses during the storage and transportation stages. Green mold, a postharvest rot of citrus fruit caused by Penicillium digitatum, is one of the most serious fungal diseases. In this study, the antagonist strain DH-4 was identified as Bacillus amyloliquefaciens according to morphological observation and 16S ribosomal DNA analysis.

Comparative transcriptional profiling of orange fruit in response to the biocontrol yeast Kloeckera apiculata and its active compounds.

Background: The yeast Kloeckera apiculata strain 34-9 is an antagonist that shows biological control activity against the postharvest fungal pathogens of citrus. An antifungal compound, 2-phenylethanol (PEA), has been identified from the extract of K. apiculata.

Mechanisms of action for 2-phenylethanol isolated from Kloeckera apiculata in control of Penicillium molds of citrus fruits.

Background: Green and blue mold decay, caused by Penicillium digitatum and P. italicum, respectively, are important postharvest diseases of citrus. Biocontrol by microbes is an alternative to synthetic fungicide application.

Phenylethanol promotes adhesion and biofilm formation of the antagonistic yeast Kloeckera apiculata for the control of blue mold on citrus.

The yeast Kloeckera apiculata strain 34-9 is an antagonist with biological control activity against postharvest diseases of citrus fruit. In a previous study it was demonstrated that K. apiculata produced the aromatic alcohol phenylethanol.

Farnesol induces apoptosis and oxidative stress in the fungal pathogen Penicillium expansum.

This study was conducted to evaluate the effect of farnesol (FOH) on the growth of P. expansum. The viability of P.