Publications by authors named "ChaoPing Lou"
Article Synopsis
- A novel microfluidic chip was developed for isolating DNA or RNA from as few as one bacterial cell in very small sample volumes (1 μl to 125 nl), making it useful for clinical and biological applications.
- The process uses phase partitioning with an organic layer to significantly enhance nucleic acid recovery yields, achieving up to 10 times more RNA or DNA compared to conventional methods.
- The chip allows for on-chip quantitative PCR analysis directly in the microwells, facilitating high-throughput extraction and analysis of nucleic acids from single bacterial cells, ideal for automated pathogen detection and gene expression studies.
Fast detection of waterborne pathogens is important for securing the hygiene of drinking water. Detection of pathogens in water at low concentrations and minute quantities demands rapid and efficient enrichment methods in order to improve the signal-to-noise ratio of bio-sensors. We propose and demonstrate a low cost and rapid method to fabricate a multi-layer polymeric micro-sieve using conventional lithography techniques.
View Article and Find Full Text PDFA high-flux metallic micro/nano-filtration membrane has been fabricated and validated for isolation of waterborne pathogens from drinking water. Obtained membrane with smooth surface and perfectly ordered pores was achieved by a high yield and cost effective multilevel lithography and electroplating technique. The micro-fabricated membrane was also strengthened with an integrated back-support, which can withstand a high pressure during filtration.
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