[Homozygous CFAP65 mutation induces multiple morphological abnormalities of sperm flagella: A preliminary genetic study].

Objective: To search for the possible pathogenic genes for multiple morphological anomalies of sperm flagella (MMAF).

Methods: We performed whole exome sequencing (WES) of a typical case of MMAF and analyzed its possible pathogenic genes. We examined the semen sample from the patient and identified the ultrastructural characteristics of the sperm flagella under the scanning electron and transmission electron microscopes, and analyzed the expression pattern of cilia and flagela-associated protein 65 (CFAP65) in spermatogenesis by immunofluorescence assay.

Drug-free and non-crosslinked chitosan scaffolds with efficient antibacterial activity against both Gram-negative and Gram-positive bacteria.

Treatment of oral pathogens is important for both oral and systemic health. The antimicrobial activity of chitosan (CS)-based scaffolds either loading antibiotics or compositing with other agents are well documented. However, the intrinsic antibacterial activity of CS scaffolds alone has never been reported.

Understanding the sheet size-antibacterial activity relationship of graphene oxide and the nano-bio interaction-based physical mechanisms.

The antibiotics-independent antimicrobial activity of graphene oxide (GO) is of great importance since antibiotic therapy is facing great challenges from drug resistance. However, the relations of GO size with its antimicrobial activity and how the size regulates the antibacterial mechanisms are still unknown. Herein, we fabricated four GO suspensions with different sizes and demonstrated the parabolic relationship between GO size and its antibacterial activity against the Gram-positive cariogenic bacterium Streptococcus mutans.

Digestive Enzyme Corona Formed in the Gastrointestinal Tract and Its Impact on Epithelial Cell Uptake of Nanoparticles.

The fate of intravenously injected nanoparticles (NPs) is significantly affected by nano-protein interaction and corona formation. However, such an interaction between NPs and digestive enzymes occurring in the gastrointestinal tract (GIT) and its impacts on epithelial cell uptake are little known. We synthesized the poly(3-hydroxybutyrate- co-3-hydroxyhexanoate)-based cationic NPs (CNPs) and investigated the CNP-digestive enzyme interaction and its effect on the cellular uptake.

Antibiofilm effect of drug-free and cationic poly(D,L-lactide-co-glycolide) nanoparticles via nano-bacteria interactions.

Aim: Recently, nano-bio interactions and their biomedical impacts have drawn much attention, but nano-bacteria interaction and its function are unknown. Herein, we aim to synthesize drug-free and cationic nanoparticles (CNPs) and investigate CNP-bacteria interaction and its antibiofilm effect.

Materials & Methods: The bioactivity of CNPs against Streptococcus mutans was examined by colony-forming units counting and scanning electron microscopy.

Organic composite-mediated surface coating of human acellular bone matrix with strontium.

Acellular bone matrix (ACBM) provides an osteoconductive scaffold for bone repair, but its osteoinductivity is poor. Strontium (Sr) improves the osteoinductivity of bone implants. In this study, we developed an organic composite-mediated strontium coating strategy for ACBM scaffolds by using the ion chelating ability of carboxymethyl cellulose (CMC) and the surface adhesion ability of dopamine (DOPA).

Concentration-dependent protein adsorption at the nano-bio interfaces of polymeric nanoparticles and serum proteins.

Aim: A comprehensive understanding of nanoparticle (NP)-protein interaction (protein corona formation) is required. So far, many factors influencing this interaction have been investigated, like size and ζ potential. However, NPs exposure concentration has always been ignored.

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas.

Background: WRAP53, including α, β and γ isoforms, plays an important role not only in the stability of p53 mRNA, but also in the assembly and trafficking of the telomerase holoenzyme. It has been considered an oncogene and is thought to promote the survival of cancer cells. The aim of this study was to detect the role of TCAB1 (except WRAP53α) in the occurrence and development of head and neck carcinomas.

Effects of combined exogenous dextranase and sodium fluoride on Streptococcus mutans 25175 monospecies biofilms.

Purpose: To investigate the effects of exogenous dextranase and sodium fluoride on a S. mutans monospecies biofilm.

Methods: S.

Preformed albumin corona, a protective coating for nanoparticles based drug delivery system.

The non-specific interaction between nanoparticles (NPs) and plasma proteins occurs immediately after NPs enter the blood, resulting in the formation of the protein corona that thereafter replaces the original NPs and becomes what the organs and cells really see. Consequently, the in vivo fate of NPs and the biological responses to the NPs are changed. This is one substantial reason for the two main problems of the NPs based drug delivery system, i.

The poor osteoinductive capability of human acellular bone matrix.

Demineralized bone matrix (DBM) has extensive clinical use for bone regeneration because of its osteoinductive and osteoconductive aptitude. It is suggested that the demineralization process in bone matrix preparation is influential in maintaining osteoinductivity; however, relevant investigations, especially into the osteoinductivity of acellular bone matrix, are not often performed. This study addressed the osteoinductive capability of human acellular cancellous bone matrix (ACBM) after subcutaneous implantation in a rat model.

Crocin inhibits proliferation and nucleic acid synthesis and induces apoptosis in the human tongue squamous cell carcinoma cell line Tca8113.

Background: Cancer chemoprevention is a proven effective strategy for oral squamous cell carcinoma (OSCC). The present study was designed to investigate the effects of crocin, a potential chemopreventive agent, on growth and DNA and RNA content in a human tongue squamous cell carcinoma cell line, Tca8113.

Methods: Tca8113 cells were treated with crocin for 24, 48, 72, and 96 h at concentrations of 0.

[Effect of astragalus membranaceus on the proliferation, osteogenic capacity and structure of periodontal ligament cells in vitro].

Objective: To investigate the effect of Astragalus membranaceus (APS) on the proliferation, osteogenic capacity and structure of periodontal ligament cells (PDLCs) in vitro.

Methods: PDLCs were cultured in vitro with APS of 0.08, 0.

[Effects of different concentrations of ethylenediamine tetraacetic acid paste on removing root canal smear].

Objective: To evaluate the effect of different concentrations of ethylenediamine tetraacetic acid (EDTA) paste on the removing root canal smear layers and the degrees of erosion on the surface of the root canal walls at the different portions of canal.

Methods: Sixty human teeth with single root were instrumented using step-back technique, then were divided into six groups and treated with different concentrations EDTA paste and NaCLO solution. Group A: 0.

[Expression of human VIGILIN N terminus and observation of subcellular localization].

Objective: To express human VIGILIN N terminus gene in E. coli, prepare the polyclonal antibody and study the subcell localization of human VIGILIN.

Methods: The N-terminal sequence of human VIGILIN was amplified by PCR and cloned into the polyclonal site of pGEX-4T-2 expression vector.

[Therapeutic effects of hTR-siRNA adenovirus on human cervical cancer xenografts in nude mice].

Objective: To study the anti-tumor effects and its mechanism of hTR-siRNA adenovirus on human cervical cancer in vivo.

Methods: The in vivo model of human cervical cancer was established by the subcutaneous inoculation of HeLa cells at the right armpit of BALB/c nu/nu mice. After successful implantation, the mice were randomized into four groups, in which the mice were intratumorally injected with 0.

[The relationship in growth between A.actinomycetemcomitans and cariogenic bacteria in vitro].

Purpose: To investigate the correlation in growth between A.actinomycetemcomitans and cariogenic bacteria of S.mutans, L.

[Construction of a reverse-subtracted cDNA library for differentially expressed genes in rat liver of prothrombotic state].

Objective: To inquire into the mechanism of prothrombotic state (PTS) and the roles of liver therein by constructing a reverse-subtracted cDNA library for differentially expressed genes in rat liver of PTS.

Methods: The reverse-subtracted cDNA library for differentially expressed genes in rat liver of PTS was constructed by suppression subtractive hybridization. The rat model of PTS was induced by a high-carbohydrate diet.