The immune-related fatty acids are responsive to CO driven seawater acidification in a crustacean brine shrimp Artemia sinica.

The gradual increase of CO concentration in the atmosphere, absorbed by the ocean surface water through air to sea equilibration termed ocean acidification (OA), leads to the decline of pH in seawater. It is not clear so far how the composition of fatty acids, particular the immune-related, in marine crustacean and the subsequent energy supply in marine ecosystem are affected by OA. The brine shrimp Artemia sinica is an open and common feed that provide essential fatty acids for mariculture.

Differential protein expression using proteomics from a crustacean brine shrimp (Artemia sinica) under CO-driven seawater acidification.

Gradually increasing atmospheric CO partial pressure (pCO) has caused an imbalance in carbonate chemistry and resulted in decreased seawater pH in marine ecosystems, termed seawater acidification. Anthropogenic seawater acidification is postulated to affect the physiology of many marine calcifying organisms. To understand the possible effects of seawater acidification on the proteomic responses of a marine crustacean brine shrimp (Artemia sinica) three groups of cysts were hatched and further raised in seawater at different pH levels (8.

Detrimental effect of CO2-driven seawater acidification on a crustacean brine shrimp, Artemia sinica.

The effects of the decline in ocean pH, termed as ocean acidification due to the elevated carbon dioxide in the atmosphere, on calcifying organisms such as marine crustacean are unclear. To understand the possible effects of ocean acidification on the physiological responses of a marine model crustacean brine shrimp, Artemia sinica, three groups of the cysts or animals were raised at different pH levels (8.2 as control; 7.

Mechanisms of apple polyphenols-induced proliferation inhibiting and apoptosis in a metastatic oral adenoid cystic carcinoma cell line.

Adenoid cystic carcinoma (ACC) is characterized by intensive local invasion and high incidence of distant metastases. Conventional chemotherapy for ACC produces a poor result. We aimed to evaluate the effect of apple polyphenols (APs), a novel nutraceutical agent, on the proliferation and apoptosis levels in a metastatic oral ACC cell line.

[Characterization of growth and proliferation in a telomerase-immortalized ameloblastoma cell line].

Objective: To establish and characterize the cell line of ameloblastoma (AM) by transfection with human telomerase reverse transcriptase (hTERT).

Methods: Primary cultures of AM cells were infected with a retroviral vector encoding hTERT. Infected cells were selected and checked by immunocytochemistry (ICC), in vitro proliferation, reverse transcriptase polymerase chain reaction (RT-PCR), senescence associated beta galactosidase staining (SA-beta-Gal staining), telomerase activity assay.

Three-dimensional CT angiography for the diagnosis and assessment of arteriovenous malformations in the oral and maxillofacial region.

Objective: The purpose of this study was to evaluate the diagnostic performance of three-dimensional computed tomography angiography (3D-CTA) for arteriovenous malformations (AVMs) in the oral and maxillofacial region.

Materials And Methods: Sixty four-slice spiral CT angiography of oral or maxillofacial region was performed in 8 patients with surgically proven arteriovenous malformations. The morphologic features, size, location, boundary, and feeding and draining vessels of lesions were reviewed.

Immortalization of ameloblastoma cells via reactivation of telomerase function: Phenotypic and molecular characteristics.

Ameloblastoma (AM) is recognized as a benign tumour but locally invasive with a high risk of recurrence. In vitro model systems for studying AM are limited due to the fact that AM cells grow poorly and begin to senesce early. Japanese researchers have reported the construction of an AM cell line, AM-1, by exposing cells to human papillomavirus 16 (HPV16) but retaining the potential of transformation.

[Isolation, culture and identification of rat buccal mucosa stem cells].

Objective: To explore a method for isolation and culture Dispase II and Trypsin-EDTA. Cells were seeded onto mitomycin C-treated of rat buccal mucosa stem cells and to identify the stem cells.

Methods: Epithelial cell mass were obtained by digesting rat buccal mucosa with 3T3 Swiss albino layer and cultured in DMEM for 24 hours, followed by K-SFM culturing.