[Construction of eukaryotic expressing vector of human P2X7 and establishment of stable transfectant cell line].

Objective: To construct eukaryotic expression vector of human P2X7gene and transfect HEK293 cells so as to establish stable HEK293 cell line.

Methods: P2X7 gene was amplified by polymerase chain reaction from the human brain P2X7 cDNA and inserted into a vector pEGFP-N1 to construct a recombinant plasmidcalled pEGFP-N1/P2X7. The correct recombinant plasmid was transfected into HEK293 cells by X-fect transfection reagent.

[Protective effect of hypoxic preconditioning on SH-SY5Y cells injured by oxygen-glucose deprivation].

Objective: To investigate the protection effects of hypoxic preconditioning(HPC) on the SH-SY5Y cell injured by oxygen-glu-cose deprivation(OGD),and to discuss the possible mechanism.

Methods: SH-SY5Y cells were randomly divided into 4 groups. In normal group,the cells were cultured without OGD treatment.

[Neuroprotective effect of progesterone on focal cerebral ischemia/reperfusion injury in rats and its mechanism].

Objective: To observe the neurological protective effects of progesterone (PROG) on focal cerebral ischemia/reperfusion injury in rats and to explore its possible mechanism.

Methods: One handred and twenty male SD rats were divided into three groups randomly: sham-operated group, middle cerebral artery occlusion ( MCAO ) group and PROG + MCAO group( n = 40). The right temporary MCAO model was established by the line-embolism method.

[Construction of acid-sensitive potassium channel-3 eukaryotic expression plasmid and its express in SH-SY5Y cells].

Objective: To construct the acid-sensitive potassium hannel-3(TASK3) eukaryotic expression plasmid and to establish a stable SH-SY5Y cell line expressing enhanced green fluorescent protein (EGFP)-tagged TASK3.

Methods: TASK3 coding region was subcloned into pEGFP-N1 plasmid to construct a recombinant vector alled pEGFP-TASK3. The correct recombinant expressing plasmid was transfected with X-feet transfection reagent to SH-SY5Y cells.

Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats.

The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery occlusion, the animals were intraperitoneally injected with hydrogen sulfide donor compound sodium hydrosulfide.