Oncologic Outcomes of Laparoscopic Radical Hysterectomy Incorporating Modified Tumor-Free Techniques.

Objective: It remains unclear whether modifying laparoscopic radical hysterectomy to adopt tumor-free principles can improve oncologic outcomes in patients with early-stage cervical cancer.

Methods: We performed a single-center retrospective cohort study of 276 patients with early-stage cervical cancer who were treated between January 2017 and January 2023, including 151 patients who underwent laparoscopic radical hysterectomy that incorporated modified tumor-free techniques (MTF group) and 125 patients who underwent conventional laparoscopic radical hysterectomy with a uterine manipulator and unprotected intracorporeal colpotomy (non-MTF group). Oncologic outcomes and perioperative results were analyzed using inverse probability treatment weighting (IPTW).

Detection of African swine fever virus antibodies using p11.5 and p14.5 protein-based indirect ELISA.

African swine fever (ASF), which is caused by the African swine fever virus (ASFV). There are currently no effective vaccines or therapies for ASF; rapid diagnosis has become an important way to control the disease. The p11.

Quantitative fluorescent detection of tetracycline in animal-derived foods using quantum dots.

Tetracycline (Tc) antibiotics, a class of synthetically produced broad-spectrum antimicrobial drugs, have been widely used in animal husbandry, leading to their widespread presence in animal-derived foods. However, misuse, overuse, and non-compliance with withdrawal periods in animal farming have resulted in excessive Tc residues in these foods, which can cause various adverse reactions in humans, induce bacterial resistance, and pose a significant threat to public health. Consequently, the detection of Tc antibiotic residues in animal-derived food has become a critical issue.

OGT mediated HDAC5 O-GlcNAcylation promotes osteogenesis by regulating the homeostasis of epigenetic modifications and proteolysis.

Background: O-GlcNAc transferase (OGT) is responsible for attaching O-linked N-acetylglucosamine (O-GlcNAc) to proteins, regulating diverse cellular processes ranging from transcription and translation to signaling and metabolism. This study focuses on the role and mechanisms of OGT in osteogenesis.

Materials And Methods: We found that OGT is downregulated in osteoporosis by bioinformatics analysis, determined its role in osteogenic differentiation by using OGT inhibitors (or OGA inhibitors) as well as conditional knockout OGT mice in and in , and explored and specific mechanisms by quantitative proteomic analysis and RNA-seq, qRT-PCR, western blotting, immunofluorescence, H&E, ALP, ARS, Masson staining, IHC, micro CT, etc.

Impacts of trophic interactions on carbon accrual in soils.

The transformation and stabilization of soil organic carbon (SOC) are important processes of global carbon (C) cycling, with implications for climate change. Much attention has been given to microbial anabolic processes driving SOC accrual. These are referred to as the soil microbial carbon pump (MCP), which emphasizes the contribution of microbial metabolism and necromass to the stable soil C pool.

Application of PACz-Based Self-Assembled Monolayer Materials in Efficient Perovskite Solar Cells.

Due to the advantages of low interface resistance, high work function, and high stability, PACz family materials have developed rapidly in p-i-n structure perovskite solar cells (PSCs) in recent years. Numerous studies have shown that PSCs prepared on the basis of PACz family materials or their derivatives as hole transport layers (HTLs) generally exhibit superior performance compared to PSCs prepared on the basis of organic HTL PTAA and inorganic HTL NiO, especially in terms of stability, demonstrating unparalleled charm. Since the application of PACz-like molecule V1036 as a HTL in PSCs in 2018, research reports on high-performance PSCs based on the PACz family HTL have been widely disseminated.

Spatiotemporal characteristics and driven forces of nitrogen flow of dairy farms in China: Based on a comprehensive model combined with production practices and embedding localized parameters.

This study developed a comprehensive nitrogen (N) flow model utilizing localized data in 2000-2019. Enhancements were conducted upon previous models: (1) variations in feed N intake of cows across different production phases was considered; (2) N emission in the Intergovernmental Panel on Climate Change (IPCC) and the European Monitoring and Evaluation Program and the European Environment Agency (EMEP/EEA) was incorporated; (3) emission factor (EF) of NH was corrected based on local climate; and (4) field application of manure was excluded from the system boundary to accommodate China's production status. The effects of farm scale (LF, ≥100 heads; SF, <100 heads) and manure management strategies (dry-cleaning and slurry) were considered.

ApoEVs Transfer Mitochondrial Component to Modulate Macrophages in Periodontal Regeneration.

Objective: Macrophages are key players in the host immune response to periodontal pathogens and tissue repair. The aim of this study was to explore the potential of apoptotic cell-derived extracellular vesicles (ApoEVs) in modulating the mitochondrial function of macrophages as a mean to enhance periodontal tissue regeneration.

Subjects And Methods: ApoEVs were extracted from periodontal ligament stem cells (PDLSCs) and characterized to observe their effects on macrophage function.

A Novel Cell- and Virus-Free SARS-CoV-2 Neutralizing Antibody ELISA Based on Site-Specific Labeling Technology.

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) led to the global spread of coronavirus disease 2019 (COVID-19), creating an urgent need for updated methods to evaluate immune responses to vaccines and therapeutic strategies. In this study, we introduce a novel cell-free, virus-free SARS-CoV-2 neutralizing antibody ELISA (NAb-ELISA), which is based on competitive inhibition of the receptor binding domain (RBD) of spike protein binding to the angiotensin-converting enzyme 2 (ACE2) receptor. In this method, site-specific biotinylated hACE2-Fc-Avi recombinant protein is immobilized onto a 96-well plate for capture, and the RBD-Fc-vHRP recombinant proteins serve as detection probes.

Development of hybrid aptamers-engineered PROTACs for degrading VEGF165 in both tumor- and vascular endothelial cells.

Tumors and angiogenesis are connected through a complex interplay. VEGF165, generated from both tumor and vascular endothelial cells, serves as a mutual benefit for both cell types. Therapeutic approaches modulating VEGF165 have been proposed as promising antitumor therapies.

AND-Gate Logic Förster Resonance Energy Transfer/Magnetic Resonance Tuning Nanoprobe for Programmable Antitumor Immunity Imaging.

Simultaneous detection of different biomarkers related to the spatiotemporally dynamic immune events is of particular importance for the accurate evaluation of antitumor immune effects. Here, we have developed an AND-gate logic dual resonance energy transfer nanoprobe (named DRET) for dynamic monitoring of programmed CD8 T cell activation and tumor cell apoptosis. Immunotherapy-induced granzyme B secretion from CD8 T cells and the subsequent caspase-3 release from apoptotic tumor cells individually activate one of the tiers of the "AND-gate" logic DRET.

Treatment of microvascular angina pectoris by activating blood circulation to remove blood stasis: A systematic review and meta-analysis.

Background: To systematically evaluate the clinical efficacy of using the method of activating blood circulation and removing blood stasis in the treatment of microvascular angina (MVA) based on the meta-analysis method.

Methods: A comprehensive search of randomized controlled trials on the treatment of MVA using the method of activating blood circulation and removing blood stasis in Chinese and English databases such as China National Knowledge Infrastructure Database, Wanfang Data Knowledge Service Platform, Vipshop Journal Resource Integration Service Platform, PubMed, Cochrane Library, Embase, Web of Science, and CBM was conducted, and the search time limit was from the establishment of each database to February 2023. All retrieved literature was screened, and data extracted according to the specified inclusion and exclusion criteria, and data analysis was completed using Revman 5.

Plant kinetochore complex: composition, function, and regulation.

The kinetochore complex, an important protein assembly situated on the centromere, plays a pivotal role in chromosome segregation during cell division. Like in animals and fungi, the plant kinetochore complex is important for maintaining chromosome stability, regulating microtubule attachment, executing error correction mechanisms, and participating in signaling pathways to ensure accurate chromosome segregation. This review summarizes the composition, function, and regulation of the plant kinetochore complex, emphasizing the interactions of kinetochore proteins with centromeric DNAs (cenDNAs) and RNAs (cenRNAs).

Interface-Strengthened Ru-Based Electrocatalyst for High-Efficiency Proton Exchange Membrane Water Electrolysis at Industrial-Level Current Density.

Developing an OER electrocatalyst that balances high performance with low cost is crucial for widely adopting PEM water electrolyzers. Ru-based catalysts are gaining attention for their cost-effectiveness and high activity, positioning them as promising alternatives to Ir-based catalysts. However, Ru-based catalysts can be prone to oxidation at high potentials, compromising their durability.

Deciphering the Intricate Control of Minerals on Deep Soil Carbon Stability and Persistence in Alaskan Permafrost.

Understanding the fate of organic carbon in thawed permafrost is crucial for predicting climate feedback. While minerals and microbial necromass are known to play crucial roles in the long-term stability of organic carbon in subsoils, their exact influence on carbon persistence in Arctic permafrost remains uncertain. Our study, combining radiocarbon dating and biomarker analyses, showed that soil organic carbon in Alaskan permafrost had millennial-scale radiocarbon ages and contained only 10%-15% microbial necromass carbon, significantly lower than the global average of ~30%-60%.

Identification and characterization of linear B-cell epitopes on African swine fever virus H171R protein.

Unlabelled: African swine fever virus (ASFV) is a highly contagious and often fatal pathogen that poses a significant threat to the swine industry worldwide. The H171R protein, a structural component of ASFV, plays crucial roles in viral assembly, host cell entry, and modulation of the host immune response. This study aimed to comprehensively characterize the linear B-cell epitopes on the H171R protein to facilitate the development of diagnostic tools and subunit vaccines against ASFV.

Design of Murine Double Minute 2 Proteolysis Targeting Chimera Degraders with a Built-In Tumor-Targeting Ability.

Proteolysis targeting chimeras (PROTACs) are heterobifunctional molecules to induce the proteasomal degradation of target proteins. Currently, there are no tumor-targeting PROTACs for modulating oncogenic murine double minute 2 (MDM2). AS1411 is a tumor-targeting aptamer that specifically recognizes nucleolin (NCL) overexpressed on the surface of tumor cells.

Identification of a novel conserved B-cell epitope in p15 of the African swine fever virus.

African Swine Fever Virus (ASFV), a highly contagious DNA virus, causes severe economic losses to the global swine industry. The ASFV p15 protein, which is found in the core shell, is essential to the assembly of viral particles. In addition, protein p15 is a candidate target for the development of diagnostic reagents for African Swine Fever (ASF) because of its excellent immunogenicity.