Publications by authors named "Changzhong Li"
Background: The aim of this study was to compare the effectiveness of two different vitrification methods and slow freezing in terms of the recovery of endocrine function, follicular morphology and proliferation, apoptosis of stromal cells, and angiogenesis after heterotopic transplantation of human ovarian tissue.
Methods: Ovarian tissue from young women aged 29 to 40 was subjected to two vitrification methods and one slow freezing method. The thawed ovarian tissue was then transplanted into nude mice and divided into three groups (VF1 group, VF2 group, SF group) according to the different freezing methods.
Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility.
Gynecol Endocrinol
December 2024
Objective: To investigate the effectiveness of gelatin/polycaprolactone (GT/PCL) membranes for restoring endometrial structure and function and fertility in a rat model of endometrial injury.
Methods: We randomized 125 female Sprague-Dawley (SD) rats to the sham, natural repair (NR), estrogen (E), GT/PCL, and E-GT/PCL groups. Except for the sham group, all rats underwent uterine curettage.
The present study aimed to evaluate the application of DNA ploidy analysis, human papillomavirus (HPV-DNA) test, and thin-prep cytologic test (TCT) for mass screening of cervical cancer (CC). A total of 13,830 patients who underwent both TCT and HPV-DNA tests from June 2021 to June 2022 were selected as study participants (10,107 patients from Shandong Provincial Hospital, 2447 patients from First People's Hospital of Tancheng County, and 1276 patients from Liaocheng People's Hospital). We also conducted a DNA ploidy analysis and compared the results.
View Article and Find Full Text PDFHiporfin photodynamic therapy for early-stage cervical cancer: A case report.
Photodiagnosis Photodyn Ther
December 2024
A 28-year-old female patient, diagnosed with stage IA1 cervical cancer, received PDT treatment with intravenous injection of Hiporfin, a HpD type photosensitizer. The patient's human papillomavirus (HPV) and cytology testing results were negative at the 3-month. At 6 months, the lesions on the cervical surface and within the cervical canal had completely resolved.
View Article and Find Full Text PDFArticle Synopsis
- - Mesenchymal stem cells (MSCs) are gaining interest in regenerative medicine due to their ability to differentiate into various cell types, regulate the immune system, and self-renew.
- - Recent studies have explored MSCs as potential treatments for several obstetric and gynecological conditions, such as premature ovarian failure and endometriosis, indicating their therapeutic promise.
- - The review discusses current research, challenges, and shortcomings in the clinical use of MSCs, highlighting the need for further studies to enhance their application in treating women's health issues.
The objective of this prospective study was to assess the efficacy of Hiporfin-photodynamic therapy (PDT) int the treatment of young women diagnosed with high-grade squamous intraepithelial lesions (HSIL) of the endocervical canal. This study included 14 patients aged 30 ± 4.2 years (range 21-37) with HSIL of the cervical canal, treated at our hospital from August 2021 to June 2023.
View Article and Find Full Text PDFThe study aimed to compare the effectiveness between Hiporfin-photodynamic therapy (PDT) and loop electrosurgical excision procedure (LEEP) in treating cervical high-grade squamous intraepithelial lesion (HSIL). We conducted a retrospective analysis of 104 women diagnosed with cervical HSIL at our hospital from April 2019 to July 2023. With 1:1 matched case-control, 52 cases received Hiporfin-PDT and 52 cases underwent LEEP.
View Article and Find Full Text PDFBackground: This study aimed to evaluate the efficacy and safety of pegylated liposomal doxorubicin (PLD) for patients with partially platinum-sensitive, platinum-resistant, or platinum-refractory ovarian cancer.
Methods: Patients with partially platinum-sensitive, platinum-resistant, or platinum-refractory ovarian cancer were recruited in this prospective, open-label, single-arm, multicenter study. Eligible patients were given 4-6 cycles of PLD (40 mg/m2 on day 1, every 4 weeks).
Article Synopsis
- Vulvovaginal candidiasis (VVC) is a common infection with a high recurrence rate, defined as having four or more episodes in a year, which poses significant clinical challenges.
- In this study, researchers analyzed vaginal samples from 31 recurrent VVC patients, 28 VVC patients, and 29 healthy women, finding similarities in symptoms but noting increased swelling in the VVC group.
- The analysis revealed changes in the vaginal microbiota, including decreased beneficial bacteria and increased bacteria associated with bacterial vaginosis, highlighting the potential role of metabolites like L-glutamate in recurring infections, which may inform future treatment strategies.
Article Synopsis
- The study aims to validate the 2019 US risk-based guidelines for cervical cancer screening specifically for Chinese women using data from over 10,000 cases.
- Analysis revealed that the guidelines effectively recommended appropriate actions, with high sensitivity (87.1%) and specificity (82.5%) for detecting advanced cervical lesions (CIN2+ and CIN3+) while also effectively identifying lower-risk cases.
- Overall, the findings suggest that the US risk-based guidelines are applicable and reliable for cervical cancer screening in China, indicating their potential to enhance public health efforts in this area.
Article Synopsis
- - Copper (Cu) is a crucial trace element that plays important roles in biological functions like antioxidant defense and cellular respiration, with a new concept called cuproptosis explaining how it can induce programmed cell death.
- - Cuproptosis specifically targets certain enzymes in the tricarboxylic acid cycle, ultimately affecting cancer dynamics by promoting tumor growth and spread in gynecological cancers like cervical and ovarian cancers.
- - The review highlights the importance of understanding Cu homeostasis and cuproptosis in gynecological malignancies, discussing their potential for prognosis prediction and the therapeutic use of Cu ionophore therapy.
Uterine arteriovenous malformation: a case report with proposal for pathogenesis.
BMC Womens Health
September 2024
Article Synopsis
- - Uterine arteriovenous malformation (UAM) is a rare condition that can lead to severe and potentially life-threatening bleeding, but there is no agreed-upon treatment guideline due to its rarity and varying patient needs.
- - The text shares the case of an elderly woman with a unique presentation of UAM, emphasizing the complexities involved in diagnosing and treating this condition.
- - The authors also suggest a hypothesis regarding the origin or development (pathogenesis) of UAM, based on their clinical experience with the diagnosis and treatment.
Article Synopsis
- AICyte's study evaluated its potential to reduce workload in cervical cytology screening by implementing a 50% negative cutoff value across 163,848 cases collected from various hospitals in China.
- Results showed that 98.80% of cases marked as not needing further review by AICyte had negative original diagnoses, with high sensitivity and negative predictive values for identifying lesions.
- The findings suggest that using AICyte could streamline diagnostic processes and decrease the burden on pathologists by categorizing certain cases as negative without further review.
HiPorfin photodynamic therapy for vaginal high-grade squamous intraepithelial lesion.
Arch Gynecol Obstet
August 2024
Purpose: We aimed to evaluate the efficacy and safety of HiPorfin-photodynamic therapy (PDT) in women with vaginal high-grade squamous intraepithelial Lesion (HSIL).
Methods: Retrospective analysis of eighteen patients with vaginal HSIL received HiPorfin-PDT between June 2019 and May 2023. Illumination with a 630-nm laser light was applied to the lesions 48-72 h after intravenous injection of 2 mg/kg HiPorfin.
N6-methylated adenosine (mA) is a crucial RNA modification in eukaryotes, particularly in cancer. However, its role in cervical cancer (CC) is unclear. We aimed to elucidate the part of mA in CC by analyzing methyltransferase-like 3 (METTL3) expression, identifying downstream targets, and exploring the underlying mechanism.
View Article and Find Full Text PDFRatiometric near-infrared fluorescent pH probes with various p values were innovatively designed and synthesized based on cyanine with a diamine moiety. The photochemical properties of these probes were thoroughly evaluated. Among the series, IR-PHA exhibited an optimal p value of approximately 6.
View Article and Find Full Text PDFWe present a rare case of a female pelvic solitary fibrous tumor unsuccessfully resected using single-port laparoscopy, requiring conversion to laparotomy. Although the resection was successful, the surgical approach could have been improved. For large tumors, minimally invasive results are possible with flexible choices of equipment and incision position.
View Article and Find Full Text PDFFully grown oocytes have the natural ability to transform 2 terminally differentiated gametes into a totipotent zygote representing the acquisition of totipotency. This process wholly depends on maternal-effect factors (MFs). MFs stored in the eggs are therefore likely to be able to induce cellular reprogramming to a totipotency state.
View Article and Find Full Text PDFArticle Synopsis
- The study explores the use of the AICyte assistive AI system for improving cervical cancer screenings by generating diagnostic images and assessments from ThinPrep Pap slides.
- Pathologists using AICyte showed a significant reduction in reading time—from an average of 180 seconds to about 22 seconds per case—while maintaining high sensitivity and improving specificity for detecting serious lesions.
- AICyte demonstrated exceptional performance at a 50% negative cutoff, achieving nearly perfect sensitivity and missing no high-grade lesions, showcasing its potential to enhance diagnostic accuracy and efficiency in pathology.
Successful pregnancy and delivery after HiPorfin photodynamic therapy for cervical high-grade squamous intraepithelial lesion.
Photodiagnosis Photodyn Ther
April 2024
Objective: The study analyzed HiPorfin-Photodynamic therapy (PDT) for young women with cervial high-grade squamous intraepithelial lesion (HSIL) and evaluated the impact of PDT on their pregnancy and delivery.
Methods: Retrospective analysis of 4 patients (21-33 years old) were treated with HiPorfin® (2 mg/kg) PDT in 2019-2022. 1 patient suffered from cervical intraepithelial neoplasia (CIN)Ⅱ and 3 patients from CIN Ⅲ.
Biomaterial scaffolds have been used successfully to promote the regenerative repair of small endometrial lesions in small rodents, providing partial restoration of gestational function. The use of rabbits in this study allowed us to investigate a larger endometrial tissue defect and myometrial injury model. A gelatin/polycaprolactone (GT/PCL) gradient-layer biofilm was sutured at the defect to guide the reconstruction of the original tissue structure.
View Article and Find Full Text PDFArticle Synopsis
- There is a lack of population-based research on how HPV infection affects the vaginal environment, which can influence the risk of long-term HPV infections.
- The study aims to explore the relationship between vaginal microbiota and vaginal metabolites in response to changes in HPV infection status.
- Findings suggest that analyzing the vaginal metabolome could be a more effective way to assess the effects of HPV infection on the vaginal microenvironment than looking at vaginal microbiota alone.
Background: Abnormal stromal-epithelial cell communication is a pathogenic mechanism in endometriosis, and metformin can modulate it. Insulin-like growth factor binding protein-1 (IGFBP1) plays a role in endometriosis, but the exact mechanism is unknown. IGFBP1 is reportedly a downstream target of metformin in some diseases.
View Article and Find Full Text PDFPhotodynamic therapy with HiPorfin for cervical squamous intraepithelial lesion at childbearing age.
Photodiagnosis Photodyn Ther
April 2024
Objective: To evaluate the clinical efficacy and safety of HiPorfin® photodynamic therapy (PDT) in the treatment of young women at reproductive age with high-grade squamous intraepithelial lesion (HSIL) of the cervix.
Methods: Prospective study of 41 patients aged 28.8 ± 4.
Objectives: The human papillomavirus (HPV) screening assays from Atila Biosystems, including the new AmpFire (14 type) and ScreenFire RS (13 type), were subjected to a series of validation tests.
Methods: We used a set of samples from the Chinese Multi-Site Screening Trial (previously tested with cobas 4800 and the next-generation SeqHPV) to satisfy Meijer's criteria for clinical end-point validation. We selected 556 self-collected specimens composed of 273 high-risk HPV (hrHPV) positives and 283 hrHPV negatives on the cobas 4800 and SeqHPV.