Rapid RNA detection through intra-enzyme chain replacement-promoted Cas13a cascade cyclic reaction without amplification.

The clinical methods to detect RNA viruses and disease-related RNAs suffer from time-consuming processes, high false-positive rates, or limited sensitivity. Here, we propose a strategy for rapid RNA detection through intra-enzyme chain replacement-mediated Cas13a cascade cyclic reaction without target amplification. A hairpin RNA mediator (a cleavage substrate for target-activated Cas13a) and a guiding RNA recognized by the cleavage product through intra-enzyme chain replacement were designed and optimized.

Discovery of a Novel Inhibitor Structure of Isocitrate Lyase.

Tuberculosis remains a global threat to public health, and dormant leads to long-term medication that is harmful to the human body. isocitrate lyase (ICL), which is absent in host cells, is a key rate-limiting enzyme of the glyoxylic acid cycle and is essential for the survival of dormant . The aim of this study was to evaluate natural compounds as potential ICL inhibitors through docking and experimental verification.

One-pot synthesis of stable and functional hydrophilic CsPbBr perovskite quantum dots for "turn-on" fluorescence detection of .

All-inorganic CsPbBr perovskite quantum dots (QDs) are widely studied owing to their excellent optoelectronic properties; however, they are usually hydrophobic and unstable in water and thus their biomedical applications are seriously limited. In this study, stable and hydrophilic CsPbBr QDs functionalized with carboxyl groups (CsPbBr-COOH QDs) were prepared in one-pot with the aid of new ligands amino-poly(ethylene glycol)-carboxyl and perfluorooctyltriethoxylsilane. The aqueous solution of CsPbBr-COOH QDs maintained the initial fluorescence intensity after 8 days of storage; the free carboxyl groups on the surface of CsPbBr-COOH QDs were covalently conjugated with amino-terminal DNA to construct CsPbBr QDs-DNA probes for subsequent application.