Impact of nitrogen addition on the chemical properties and bacterial community of subtropical forests in northern Guangxi.

Introduction: In recent years, nitrogen deposition has constantly continued to rise globally. However, the impact of nitrogen deposition on the soil physicochemical properties and microbial community structure in northern Guangxi is still unclear.

Methods: Along these lines, in this work, to investigate the impact of atmospheric nitrogen deposition on soil nutrient status and bacterial community in subtropical regions, four different nitrogen treatments (CK: 0 gN m a, II: 50 gN m a, III: 100 gN m a, IV: 150 gNm a) were established.

Exogenous IL-4-expressing bone marrow mesenchymal stem cells for the treatment of autoimmune sensorineural hearing loss in a guinea pig model.

Bone marrow mesenchymal stem cells (BMSCs) expressing recombinant IL-4 have the potential to remediate inflammatory diseases. We thus investigated whether BMSCs expressing exogenous IL-4 could alleviate autoimmune sensorineural hearing loss. BMSCs isolated from guinea pigs were transfected with recombinant lentivirus expressing IL-4.

Experimental study of local inner ear gene therapy for controlling autoimmune sensorineural hearing loss.

This study aimed to investigate the efficacy of gene therapy for treating autoimmune sensorineural hearing loss (ASHL) via local administration of a recombinant adenovirus vector containing the Fas ligand or interleukin IL-10 gene. Guinea pigs were divided into four groups, with different microinjections in the scala tympani. Group A were injected with FasL-EGFP, B with IL-10-EGFP, C with EGFP, and D with artificial perilymph.

Auditory function in women with autoimmune inner ear diseases and their offspring.

Objective: The precise cause of congenital sensorineural hearing loss (CSNHL) is unclear in many cases. In a previous study we found that offspring from guinea pigs with autoimmune sensorineural hearing loss (ASNHL) exhibited signs of SNHL. Here we studied women with autoimmune inner ear diseases (AIED) and their offspring.

[Analysis of the main components of inner ear antigens inducing autoimmune Meniere's disease in guinea pigs].

Objective: To investigate the main components of inner ear antigens inducing autoimmune Meniere's disease (AIMD) in guinea pigs.

Methods: The guinea pigs were immunized with isologous crude inner ear antigens (ICIEAg). Then, the hearing function was measured with auditory brainstem response (ABR), the vestibular function was measured with electronystagmography (including spontaneous nystagmus and caloric test), and inner ear histopathological changes were observed by inner ear celloidin section with haematoxylin-eosin staining and observed under light microscope.

[Epidemiologic study on hearing impairment and ear diseases in old people].

Objective: To investigate the prevalence of hearing impairment and ear diseases in old people and provide scientific data for drawing up the prevention and treatment strategies.

Methods: Using the probability proportion to size (PPS) method, 1261 people over 60 years were investigated in 40 clusters in Jiangsu Province with the WHO protocol.

Results: The prevalence of hearing impairment was 58.

[Inner ear physiological function and pathological morphology in the offsprings of sensorineural hearing loss female guinea pigs induced by homogeneous inner ear antigens immunizing].

Objective: To explore if the autoimmune of anti-labyrinth tissues acts as one of pathogenic cause by observing the inner ear physiological functions and pathological morphology changes of offspring of autoimmune sensorineural hearing loss (ASHL) female guinea pig.

Methods: The pregnant guinea pigs were immunized with homogeneous inner ear antigens (HIEAg), then, the hearing function were measured with EcochG [inspecting items including acoustic nerve compound action potential (cAP), summation potential (-SP) and cochlear microphone potential (CM)], while the vestibular function were measured with electronystagmography (inspecting items including spontaneous nystagmus and caloric test), inner ear Celloidin section with haematoxylin-eosin dyeing and being inspected under light microscope. The special antibodies in serum and special lymphocyte immune reaction were measured with ELISA and 3H-TdR intermingling lymphocyte transform test in all female guinea pigs and their offspring guinea pigs.

[Endoscopic technique utilized into the selective vestibular neurotomy by retrosigmoid approach].

Objective: To investigate the micro-wound technique and the signification of reducing brain tissue oppression and complication at the surgery with utilizing endoscopic technique during the operation of selective vestibular neurotomy (SVNT) by retrosigmoid approach.

Methods: Twelve patients were preceded the SVNT by retrosigmoid approach (as routine operation group, ROG), and nine patients were utilized endoscope for review and neurotomy during the process of the SVNT by retrosigmoid approach (as endoscopic operation group, EOG). All of them were examined by auditory level, vestibular function, facial nerve function and observed of clinical situation (including complication and equilibrium function and so on) before and after operation.

[The clinical and pathological changes following anterior-ethmoid neurotomy in treating perennial allergic rhinitis with plasma].

Objective: To research the therapy effect of perennial allergic rhinitis (PAR) with anterior-ethmoid neurotomy, and to observe the pathological changes of SP nerve fibres.

Method: In 46 cases (neurotomy group, NG) of PAR anterior-ethmoid neurotomy were performed, the surgery effect was evaluated according to the Chinese Hai Kou allergic rhinitis diagnosis and curative access standard (1997) and compared with 17 cases simulating operation without neurotomy (non-neurotomy group, NNG) by clinical symptoms controlled counting-scores. The substance P in nasal mucous membrane of NG was observed with immunohistochemical test, meanwhile the square of positive-stained nerve fibres on the sections were measured with a quantitative analysis method after and before operation, which also were compared with that of 14 cases patients without rhinopathy (normal controlled group, NCG).

[The endoscopic technique utilized in removal process of acoustic neuroma by retrosigmoid approach].

Objective: Research into the technique and signification of reducing remnant tumor with utilizing endoscopic technique during removal of acoustic neuroma.

Method: 15 patients proceeded the removal of acoustic tumor through retrosigmoid approach (as routine retrosigmoid-approach operation group, RRSO-G), 11 patients utilized endoscope for inspecting and eliminating remain of tumor during the proceed of the removal of acoustic neuroma through retrosigmoid approach (as retrosigmoid-approach operation combined using endoscope, RSOCE-G), all of them were examined of auditory level, vestibular function, facial nerve function, and MRI before and three months after operation.

Result: The remain tumor were found in 3 cases of RRSO-G and no any one in RSOCE-G after operation.