Non-Additive Gene Expression in Carbon and Nitrogen Metabolism Drives Growth Heterosis in Populus deltoides.

Growth heterosis is crucial for Populus deltoides breeding, a key industrial-timber and ecological-construction tree species in temperate regions. However, the molecular mechanisms underlying carbon (C)-nitrogen (N) metabolism coordination in regulating growth heterosis remain unclear. Herein high-hybrids of P.

The Trx-Prx redox pathway and PGR5/PGRL1-dependent cyclic electron transfer play key regulatory roles in poplar drought stress.

Understanding drought resistance mechanisms is crucial for breeding poplar species suited to arid and semi-arid regions. This study explored the drought responses of three newly developed 'Zhongxiong' series poplars using integrated transcriptomic and physiological analyses. Under drought stress, poplar leaves showed significant changes in differentially expressed genes (DEGs) linked to photosynthesis-related pathways, including photosynthesis-antenna proteins and carbon fixation, indicating impaired photosynthetic function and carbon assimilation.

Effects of stand age and soil microbial communities on soil respiration throughout the growth cycle of poplar plantations in northeastern China.

Introduction: Many studies have identified stand age and soil microbial communities as key factors influencing soil respiration (Rs). However, the effects of stand age on Rs and soil microbial communities throughout the growth cycle of poplar ( cv.'I-214') plantations remain unclear.

Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.

Background: The interaction between soil characteristics and microbial communities is crucial for poplar growth under different planting densities. Yet, little is understood about their relationships and how they respond to primary environmental drivers across varying planting densities.

Results: In this study, we investigated poplar growth metrics, soil characteristics, and community assembly of soil bacterial and fungal communities in four poplar genotypes (M1316, BT17, S86, and B331) planted at low, medium, and high densities.

RNA-seq reveals the gene expression in patterns in Populus × euramericana 'Neva' plantation under different precision water and fertilizer-intensive management.

Background: Populus spp. is a crucial fast-growing and productive tree species extensively cultivated in the mid-latitude plains of the world. However, the impact of intensive cultivation management on gene expression in plantation remains largely unexplored.

Unraveling the lncRNA-miRNA-mRNA Regulatory Network Involved in Poplar Coma Development through High-Throughput Sequencing.

Poplar coma, the fluff-like appendages of seeds originating from the differentiated surface cells of the placenta and funicle, aids in the long-distance dispersal of seeds in the spring. However, it also poses hazards to human safety and causes pollution in the surrounding environment. Unraveling the regulatory mechanisms governing the initiation and development of coma is essential for addressing this issue comprehensively.

Intercropping changed the soil microbial community composition but no significant effect on alpha diversity.

Introduction: Enhancing the planning of the forest-agricultural composite model and increasing the efficiency with which forest land is utilized could benefit from a thorough understanding of the impacts of intercropping between forests and agriculture on soil physicochemical properties and microbial communities.

Methods: × cv. and intercrop soils, along with their corresponding monocrops, were used in this study's llumina high-throughput sequencing analysis to determine the composition and diversity of soil bacterial and fungal communities.

Impacts of litter microbial community on litter decomposition in the absence of soil microorganisms.

Unlabelled: What is the effect of phyllosphere microorganisms on litter decomposition in the absence of colonization by soil microorganisms? Here, we simulated the litter standing decomposition stage in the field to study the differences in the composition and structure of the phyllosphere microbial community after the mixed decomposition of × and var. litter. After 15 months of mixed decomposition, we discovered that litters that were not in contact with soil had an antagonistic effect (the actual decomposition rate was 18.

Analysis of Growth Trajectories and Verification of Related SNPs in .

As an important timber genus with high economic and ecological values, is a model for dissecting the genetic architecture of growth traits in perennial forest trees. However, the genetic mechanisms of longitudinal growth traits in poplar remain incompletely understood. In this study, we conducted longitudinal genetic analysis of height and diameter at breast height (DBH) in eleven-year poplar clones using ultra-deep sequencing datasets.

The Changes of Phyllosphere Fungal Communities among Three Different spp.

As an ecological index for plants, the diversity and structure of phyllosphere microbial communities play a crucial role in maintaining ecosystem stability and balance; they can affect plant biogeography and ecosystem function by influencing host fitness and function. The phyllosphere microbial communities reflect the immigration, survival, and growth of microbial colonists, which are influenced by various environmental factors and leaves' physical and chemical properties. This study investigated the structure and diversity of phyllosphere fungal communities in three different spp.

PRMD: an integrated database for plant RNA modifications.

The scope and function of RNA modifications in model plant systems have been extensively studied, resulting in the identification of an increasing number of novel RNA modifications in recent years. Researchers have gradually revealed that RNA modifications, especially N6-methyladenosine (m6A), which is one of the most abundant and commonly studied RNA modifications in plants, have important roles in physiological and pathological processes. These modifications alter the structure of RNA, which affects its molecular complementarity and binding to specific proteins, thereby resulting in various of physiological effects.

Regional testing of triploid hybrid clones of populus tomentosa.

Background: Triploid Populus tomentosa, a timber tree species, has been widely planted in northern China owing to its potential high yields and high wood quality. Though genetic variances in growth traits and wood properties have been reported across several planting sites, regional testing of triploid hybrid clones of P. tomentosa has not been conducted on a large scale.

Heterologous expression of Zygophyllum xanthoxylon zinc finger protein gene (ZxZF) enhances the tolerance of poplar photosynthetic function to drought stress.

The ZxZF transcription factor (TF) of Zygophyllum xanthoxylon (Bunge) Maxim, an extremely drought-resistant woody plant, is a CH zinc finger protein. Studies have shown that CH zinc finger proteins play important roles in activating stress-related genes and enhancing plant resistance. However, their function in regulating plant photosynthesis under drought stress is not well understood.

Differences in phyllosphere microbiomes among different spp. in the same habitat.

Introduction: The above-ground parts of terrestrial plants are collectively known as the phyllosphere. The surface of the leaf blade is a unique and extensive habitat for microbial communities. Phyllosphere bacteria are the second most closely associated microbial group with plants after fungi and viruses, and are the most abundant, occupying a dominant position in the phyllosphere microbial community.

Identification and Functional Prediction of CircRNAs in Leaves of F1 Hybrid Poplars with Different Growth Potential and Their Parents.

Circular RNAs (CircRNAs) regulate plant growth and development; however, their role in poplar heterosis is unclear. We identified 3722 circRNAs in poplar leaves, most of which were intergenic (57.2%) and exonic (40.

root exudates are associated with rhizosphere microbial communities and symbiotic patterns.

Introduction: Microbial communities in the plant rhizosphere are critical for nutrient cycling and ecosystem stability. However, how root exudates and soil physicochemical characteristics affect microbial community composition in rhizosphere is not well understood.

Methods: This study measured soil physiochemistry properties and root exudates in a representative forest consists of four species.

Litter mixing promoted decomposition rate through increasing diversities of phyllosphere microbial communities.

Decomposition of forest litter is an essential process for returning nutrients to the soil, which is crucial for preserving soil fertility and fostering the regular biological cycle and nutrient balance of the forest ecosystem. About 70% of the land-based forest litter is made up primarily of leaf litter. However, research on the complex effects and key determinants of leaf litter decomposition is still lacking.

Verification of sap flow characteristics and measurement errors of Carr. and L. based on the liquid level equilibrium method.

This study clarified the characteristics and influencing factors of sap flow in Carr. and L., and verified the applicability of Granier's original formula for measuring the sap flow of the two species, aimed to provide a basis for the accurate assessment of tree transpiration.

The cytokinin type-B response regulator PeRR12 is a negative regulator of adventitious rooting and salt tolerance in poplar.

Adventitious root (AR) development is an ecologically and economically important biological process that maintains ecological balance, improves plant survivability, and allows for massive vegetative propagation, but its genetic mechanisms are not well understood. Here, eight Arabidopsis response regulator (ARR) genes were cloned and identified in poplar, most of which were detected in the AR, phloem, and xylem and showed remarkable induction at different time points during AR development. Subcellular localization indicated that most of these PeRR genes are in the nucleus.

Transcriptome Analysis Reveals Critical Genes and Pathways in Carbon Metabolism and Ribosome Biogenesis in Poplar Fertilized with Glutamine.

Exogenous Gln as a single N source has been shown to exert similar roles to the inorganic N in poplar 'Nanlin895' in terms of growth performance, yet the underlying molecular mechanism remains unclear. Herein, transcriptome analyses of both shoots (L) and roots (R) of poplar 'Nanlin895' fertilized with Gln (G) or the inorganic N (control, C) were performed. Compared with the control, 3109 differentially expressed genes (DEGs) and 5071 DEGs were detected in the GL and GR libraries, respectively.

Effects of three regeneration methods on the growth and bacterial community diversity of Populus × euramericana.

To study the effects of different regeneration methods on the growth and bacterial community diversity of Populus × euramericana cv. '74/76' (poplar 107), we investigated the growth of poplar 107 trees under three regeneration methods in 2017 and 2020, and sequenced the 16S rDNA V5-V7 regions in stem endophytic, root endophytic, and rhizosphere soil bacteria present in samples from the three regeneration methods using the Illumina high-throughput sequencing platform. The growth analysis showed that stump grafting regeneration (ST) and stump sprouting regeneration (SP) presented similar tree height and diameter at breast height (DBH), which were significantly lower by planted seedling regeneration (CK).

Correlation Analysis of the Bacterial Community and Wood Properties of × cv. "74/76" Wet Heartwood.

Wetwood disease of poplar limits the processing and manufacturing of poplar, and the pathogenic bacteria of wet heartwood are poorly known. We used high-throughput sequencing methods to analyze the bacterial community of the heartwood, sapwood, root tissue, and rhizosphere soil of  ×  cv. "74/76" (poplar 107) in wetwood trees and healthy trees to explore the cause of poplar wetwood disease.