A reliable, high-resolution and high-throughput genotyping method for HLA-DRB1.

Human leukocyte antigen (HLA) DNA typing is an essential part to identify a donor who may be a good match for the patients who need haematopoietic stem cells from bone marrow. Thus, fetching quickly high-resolution genotype is very important at present. However, current HLA DNA typing methods especially for HLA-DRB1 generally yielded ambiguous typing results because of high degree of heterozygosity on exome region and primer pairs design limitations, which generally amplified only exon2 of HLA-DRB1 and the position of its primer pairs is on exome region.

Characterization of 27 novel HLA class II alleles from China marrow donor program.

Twenty-seven novel human leukocyte antigen (HLA) class II alleles in Chinese Marrow Donors are described: 10 HLA-DRB1 alleles and 17 HLA-DQB1 alleles.