Associations of polymorphism of P22(phox) C242T, plasma levels of vitamin E, and smoking with coronary heart disease in China.

Background: The C242T polymorphisms of P22(phox) and plasma vitamin E have been associated with the risk of coronary heart disease (CHD) in several studies, but the results have been inconsistent. In this study, we sought to examine potential interactions between P22(phox) genotypes, plasma vitamin E concentrations, and smoking in relation to CHD risk.

Methods: We determined C242T genotype frequency in the P22(phox) gene and plasma levels of vitamin E in 565 Chinese patients with CHD and 609 control subjects.

[Relationship between GSTM1 and GSTT1 gene polymorphisms and noise induced hearing loss in Chinese workers].

Objective: To investigate the relationship of GSTM1 and GSTT1 gene polymorphisms with the development of noise induced hearing loss (NIHL) in Chinese workers.

Methods: 194 workers exposed to occupational noise were drawn as the subjects in the cross-sectional epidemiological study. According to the result of audiometry, they were divided into two groups: the NIHL group and the normal group.

[Glutathione S-transferase M1, T1 genotypes and the risk of mountain sickness].

Objective: To explore the relationship between genetic polymorphisms of glutathione S-transferase (GST) M1, T1 and susceptibility to mountain sickness.

Methods: Forty-three soldiers with acute mountain sickness and 80 healthy soldiers matching with sex/age and training under the same condition were divided into case group and control group. A multiple polymerase chain reaction method was used to detect GSTM1 and GSTT1 genes in genomic DNA isolated from peripheral blood cells from both cases and controls.

[The influence of occupational exposure and smoking on lung ventilation of coke oven workers].

Objective: To study the influence of occupational exposure and smoking on lung ventilation function of coke oven workers.

Methods: Environmental monitoring was performed on the top, side and bottom of some coke ovens. Lung ventilation function test was performed in 234 coke oven workers.

[Relationship of plasma nitric oxide concentration and the activity of nitric oxide synthase with coronary heart disease].

Objective: To study the levels of nitric oxide synthase (NOS) and nitric oxide (NO) in patients with coronary heart disease (CHD), and the factors affecting them.

Methods: Concentrations of NO and the activities of NOS in plasma in 136 patients and 206 controls using the corresponding Kits were measured. The data were analyzed using covariance and multiple linear regression analysis with SAS 8.

[Relationship between heat stress protein 70 gene polymorphisms and the risk of acute mountain sickness].

Objective: To investigate the relationship between heat stress proteins 70 (HSPs70) gene polymorphism and the risk of acute mountain sickness.

Methods: Fifty-six soldiers with acute mountain sickness and 173 soldiers without that were chosen as cases and controls. HSP70-1, HSP70-2 genotypes were analyzed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.

[Effects of mitogen activated protein kinase signal transduction pathways on heat shock protein 70 gene expression in endothelial cells exposed to benzo(a)pryene].

Objective: To investigate the effects of mitogen activated protein kinase (MAPK) signal transduction pathways on heat shock protein 70 (HSP70) gene expression in endothelial cells exposed to benzo(a)pryene (BaP).

Methods: Porcine aortic endothelial cells were pre-treated or by PD98059 (10 micro mol/L) or SB203580 (20 micro mol/L) for 1 hour, then treated with different concentrations of BaP (0, 0.1, 0.

[Role of heat shock protein 70 expression in DNA damage induced by benzo(a)pyrene].

Objective: To explore heat shock protein 70 (HSP70) expression of A549 cells and its role in DNA damage caused by benzo(a)pyrene (BaP).

Methods: Human adenocarcinoma A549 cells were cultured in vitro, exposed by different concentrations of BaP (0, 1.25, 2.

[The expression of cytochrome P450 1A1 of endothelial cells induced by benzo(a)pyrene].

Objective: To explore the effect of benzo(a)pyrene (BaP) on the expression and the activities of cytochrome P450 1A1 (CYP1A1) of porcine aortic endothelial cells.

Methods: Porcine aortic endothelial cells were cultured in vitro, and treated with different concentrations of BaP (0, 0.5, 1.