In vitro study in the endothelial cell compatibility and endothelialization of genipin-crosslinked biological tissues for tissue-engineered vascular scaffolds.

To overcome the cytotoxicity of the chemical reagents used to fix bioprostheses, genipin, a naturally occurring crosslinking agent, was used to fix biological tissues in present study. We prepared the biological vascular scaffolds through cell extraction and fixing the porcine thoracic arteries with 1% (by w/v) genipin solution for 3 days, and then examined their mechanical properties and microstructures; glutaraldehyde- and epoxy-fixed counterparts were used as controls. HUVECs were seeded on the type I collagen-coated surface of different modified acellular vascular tissues (fixed with different crosslinking agents), and the growths of HUVECs on the specimens were demonstrated by means of MTT test, the secretion of PGI2 and vWF by HUVECs on the various specimens was also measured.

Preparation and endothelialization of decellularised vascular scaffold for tissue-engineered blood vessel.

The proliferation of cells on the decellularised tissues fixed by chemical crosslinking agent is retarded for cytotoxicity of crosslinked tissues. To overcome this disadvantage, we prepared the decellularised vascular scaffold through fixing the porcine thoracic arteries with 40 mL/L ethylene glycol diglycidyl ether (EGDE), and reduced the cytotoxicity of this scaffold by treating it with lysine and coating it with type I collagen, finally endothelialized it in vitro. The EGDE-fixed porcine thoracic arteries were examined morphologically.

Effect of strontium ions on the growth of ROS17/2.8 cells on porous calcium polyphosphate scaffolds.

Preparation, characterization and cellular biocompatibility study of a series of calcium polyphosphate containing 0-100 mol% of Ca2+ replaced by Sr2+ were reported. The osteoblastic ROS17/2.8 cell line was used and seeded on the strontium-doped calcium polyphosphate (SCPP) scaffolds to estimate its optimal dose and to study its potential to support the growth of osteoblastic cells for bone tissue engineering.

[Research progresses of the synthesis and application of oligopeptide containing Arg-Gly-Asp sequence].

Arg-Gly-Asp (RGD) is one of the oligopeptides with biological activity found in recent years. It is the minimum ammonia serial on the ligand, which can be recognized by most of the integrins. It has important influence on the recognition of endothelial cell, anti-thrombus and anti-tumour function, and the therapy of burn and skin helcosis.