Advanced oxidation protein products trigger apoptosis and block epithelial-to-mesenchymal transition in crypt epithelial cells.

Advanced oxidation protein products (AOPPs) are uremic toxins. The present study aimed to investigate the effects of AOPPs on the epithelial mesenchymal transition (EMT) and apoptosis of rat crypt epithelial cells, and to assess the signaling pathways involved. The oxidized rat serum albumin was obtained by sodium hypochlorite modification as AOPPs, and the rat serum albumin (RSA) without sodium hypochlorite modification was set as the control.

[Research progress in pharmacological effects of Uncaria Hook on Alzheimer disease models].

The most common neurodegenerative disease, Alzheimer disease (AD) constitutes the majority of all senile dementia cases. Extending life expectancy contributes to the increased incidence of AD, which is a serious threat to the quality of life of the elderly. The etiology and pathogenesis of AD are not absolutely clear.

[Advance in studies on neuroprotective mechanism of Uncariae Ramulus Cum Uncis].

The Uncariae Ramulus Cum Uncis is a commonly used traditional Chinese medicine. In recent years, many studies have revealed its prominent neuroprotection function. The active ingredients in Uncariae Ramulus Cum Uncis could protect the nervous system in a multi-path and multi-target manner.

[Pattern of lymph node metastasis and extent of lymphadenectomy for distal gastric cancer].

Objective: To analyze lymph node (LN) metastasis patterns and determine the appropriate extent of LN dissection in distal-third gastric cancer.

Methods: Clinical data of 545 patients with distal third gastric cancer undergoing radical operation in the Fujian Provincial Hospital between 2001 and 2010 were analyzed retrospectively. The metastasis rate for each LN station was analyzed stratified by the depth of tumor invasion.

Discovery and validation of prognostic markers in gastric cancer by genome-wide expression profiling.

Aim: To develop a prognostic gene set that can predict patient overall survival status based on the whole genome expression analysis.

Methods: Using Illumina HumanWG-6 BeadChip followed by semi-supervised analysis, we analyzed the expression of 47,296 transcripts in two batches of gastric cancer patients who underwent surgical resection. Thirty-nine samples in the first batch were used as the training set to discover candidate markers correlated to overall survival, and thirty-three samples in the second batch were used for validation.

[Analysis of prognostic factors in lymph node-negative advanced gastric cancer patients].

Objective: To investigate the prognostic factors of lymph node-negative advanced gastric cancer patients in order to guide adjunctive therapy and surveillance tragedy.

Methods: A total of 236 advanced gastric cancer patients with no less than 12 retrieved lymph nodes and without lymph node metastasis from Fujian Provincial Hospital between 1998 and 2008 were collected retrospectively. Univariate and multivariate prognostic analysis were performed.

[Systematic analysis of cis-nested gene pairs in the human genome].

In the genome one gene, whose entire or the most part of the sequence localizes in an intronic or UTR region of another larger gene, is called nested gene. A nested gene pair consists of a host and a nested gene. Here, we conducted a systematic scanning and analysis to identify all cis-nested gene pairs and their structural features in the human genome.

[The contribution of gene conversion at the same chromosome to the HLA diversity].

HLA is the most polymorphic gene family in human genome, which is imperative for human to face numerous heterogeneous bio-molecules. Previous studies on the formation of HLA polymorphism have been focused on gene crossover. Here, we investigated the contribution of gene conversion, which is an important mechanism to generate polymorphism at shaping different patterns of HLA-DRB genes.

[Contents mensuration of total alkaloid in Uncaria rhynchophylla by acid dye colorimetry].

Objective: To investigate the method of determination of total alkaloids Uncaria rhynchophylla.

Methods: The Contents of total Alkaloid were determined by Acid dye Colorimetry.

Results: Acid dye color conditions: pH3.

Identification of new centrosome proteins by autoimmune patient sera.

Compared to other subcellular organelles, centrosome proteome can hardly be studied, due to the difficulties in separation and purification of centrosome. Auto-antisera from 6 autoimmune patients, which recognized centrosome specifically in immunofluorescence, were used to identify the corresponding centrosomal proteins. The sera were first tested by Western blot on whole cell lysate, and all bound antibodies were then eluted from each single band in Western blot membrane to assure which antibody was responsible for the centrosome specific immunofluorescence staining.

Neighboring-nucleotide effects on the mutation patterns of the rice genome.

DNA composition dynamics across genomes of diverse taxonomy is a major subject of genome analyses. DNA composition changes are characteristics of both replication and repair machineries. We investigated 3,611,007 single nucleotide polymorphisms (SNPs) generated by comparing two sequenced rice genomes from distant inbred lines (subspecies), including those from 242,811 introns and 45,462 protein-coding sequences (CDSs).