Aqueous Extracts of Kob Induce Apoptosis to Exert Anticancer Activity.

Cancer has become the leading cause of mortality since 2010 in China. Despite the remarkable advances in cancer therapy, a low survival rate is still a burden to the society. The antineoplastic activity of aqueous extracts of Kob (AECK) was measured in this study.

[Analysis and evaluation of different radial parts of Cordyceps kyushuensis by Fourier transform infrared spectroscopy].

Fourier-transform infrared spectroscopy (FTIR) and second derivative spectra were used to analyze and evaluate the different parts of Cordyceps kyushuensis Kob in the present work. The results showed that C. kyushuensis contained proteins, polysaccharides, nucleosides, lipids and other active ingredients, the single-dimensional IR spectra of the various parts were highly similar, the similarity coefficient between the cultured stroma and medium reached up to 0.

Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography.

Supercritical fluid extraction (SFE) was used to extract quinolizidine alkaloids from Sophora flavescens Ait. (Kushen). An orthogonal test L(9)(3)(4) including pressure, temperature, flow rate of CO(2) and the amount of modifier was performed to get the optimal conditions.

Synthesis and properties of cordycepin intercalates of Mg-Al-nitrate layered double hydroxides.

Layered double hydroxides (LDHs) were investigated as cordycepin delivery nanocarriers for the first time in this study. Negatively charged biomolecule-cordycepin was intercalated in the gallery spaces of [Mg-Al-NO(3)] as the charge-compensating species, which was confirmed by the results of XRD, FT-IR, TEM, CZE and electrophoretic mobility. Cell experiment suggested that the new bio-LDH nanohybrid could prevent cordycepin decomposition by adenosine deaminase.

Detection and quantification of phnE gene from oil-contaminated soil samples by competitive quantitative PCR.

The phnE gene encoding catechol 2,3-dioxygenase belonging to the meta-cleavage pathway was selected as the marker gene and was detected and quantified from soil samples by competitive quantitative PCR. A PCR primer pair was designed based on the phnE gene to amplify the target DNA bands and competitor DNA bands. The phnE gene was detected in two samples of three.

Measurement of cordycepin and adenosine in stroma of Cordyceps sp. by capillary zone electrophoresis (CZE).

Capillary zone electrophoresis (CZE) was used to separate cordycepin and adenosine, and determine their concentration in stroma of Cordyceps sp. These two active components of natural and cultured Cordyceps kyushuensis were quantitatively analyzed and compared with those of cultured Cordyceps militaris. The results showed that the CZE method is a simple, rapid and sensitive method for the measurement of cordycepin and adenosine with good repeatability.

[Study on the interaction of cordycepin and DNA].

The absorbance, fluorescence and Scatchard plots methods as well as the effect of phosphate group on the fluorescence intensity of the cordycepin-DNA-EB system were used to study the interaction of the antitumor compound cordycepin and DNA. It is obvious tiat there is hyperchromic effect and hypochromic effect with slight red shift on the subtracted UV spectrum. It proves that the adenine base of cordycepin can be inserted into the double-helix of DNA.

[A simple and effective method for extracting total RNA from domestic fungus].

With Flammulina velutipes material,an improved method was developed for extracting total RNA from domestic fungus that are rich in RNase,polyphenols, polymeric carbohydrates and proteoglycans. Phenol-chloroform-isoamyl alcohol were used twice to clear DNA and protein under higher concentration of denaturing solution and isopentanol, sodium acetate were used to precipitate RNA selectively. Pure and intact RNA can be effectively prepared by this method.

[Nucleoside from Cordyceps kyushuensis and the distribution of two active components in its different parts].

Aim: To rapidly separate and determine the nucleosides from natural and cultured Cordyceps kyushuensis Kob., and to compare the content of cordycepin and adenosine in different parts of Cordyceps kyushuensis Kob., which are the main nucleoside active components in medicinal fungus belonging to Cordyceps (Fr.

Conversion of an obligate autotrophic bacteria to heterotrophic growth: expression of a heterogeneous phosphofructokinase gene in the chemolithotroph Acidithiobacillus thiooxidans.

A plasmid pSDK-1 containing the Escherichia coli phosphofructokinase-1 gene (pfkA) was constructed, and transferred into Acidithiobacillus thiooxidans Tt-7 by conjugation. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium but the enzyme activity (18 U g-1) was lower than that in E.