Structural characterization and antioxidant activities of 2 water-soluble polysaccharide fractions purified from tea (Camellia sinensis) flower.

Unlabelled: The water-soluble crude polysaccharide tea flower polysaccharide (TFP), obtained from tea (Camellia sinensis) flower by boiling-water extraction and ethanol precipitation, was fractionated by Sephadex G-100 column chromatography, giving 2 polysaccharide fractions termed TFP-1 and TFP-2. The structural features of TFP-1 and TFP-2 were investigated by high-performance liquid chromatography (HPLC), gel-permeation chromatography (GPC), rheometer, infrared (IR) spectra, nuclear magnetic resonance (NMR) spectroscopy, atomic force microscope (AFM), and scanning electron microscope (SEM). Results indicated that TFP-1 was composed of glucose: xylose: rhamnose: galactose=1.

Isolation, Purification and Characterization of a Group of Novel Small Molecular Ribosome Inactivating Protein -- LuffinS from Seeds of Luffa cylindrica.

A group of novel RIPs--LuffinS(1), LuffinS(2), LuffinS(3) (MW about 8 kD) were purified by ammonia sulfate precipitation, CM-52 chromatography, HRLC size chromatography and Mono S FPLC. LuffinS(1), LuffinS(2) and LuffinS(3) have similar weight of about 8kD, and their N-terminal amino acid is Ala, Pro and Thr respectively. The N-terminal nine amino acid sequence of LuffinS(2) was determined as Pro-Arg-Arg-Gly-Gln-Glu-Ala-Phe-Asp.

In vitro Inhibition of Human Melanoma Cells by Immunotoxin Luffin B-Ng76.

Luffin B, a plan single-chain ribosome inactivating protein, was purified from seeds of Luffa cylindrica by Blue Sepharose CL-6B affinity chromatography. An immunotoxin was constructed with luffin B and Ng76, a monoclonal antibody to human melanoma cell M(21). Luffin B-Ng76 showed 4 000-fold more cytotoxic to target melanoma cells than free luffin B.