Publications by authors named "Chang-Mao Xu"
[Cloning and expression of D-like aspartic protease of Anisakis simplex].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
August 2012
Objective: To clone and express the full length of D-like aspartic protease gene (AsAP) of the third stage larvae of Anisakis simplex.
Methods: According to the partial information of D-like aspartic protease encoding gene of A. simplex from GenBank, specific primers were designed to amplify 3'end and 5' end of AsAP gene using rapid amplification of cDNA ends (RACE), and the full length of the D-like aspartic protease gene was obtained.