Publications by authors named "Chang-Li Zheng"
To detect the expression of miRNA-214 in human gastric cancer cell lines of BGC823, MKN45 and SGC7901, and to identify the effect of miRNA-214 on cell cycle and apoptosis of these cells. Expression of miRNA-214 in human normal gastric mucosal cell line GES-1 and human gastric cancer cell lines was detected by real-time reverse-transcription polymerase chain reaction. Antisense-miRNA-214 oligonucleotides were transfected transiently into gastric cancer cell lines to down-regulate the expression of miRNA-214.
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- Beta-catenin is often overexpressed in various cancers, including esophageal cancer, and contributes to tumor growth and cell proliferation.
- Researchers silenced beta-catenin in a specific esophageal cancer cell line using a DNA template to trigger RNA interference, which resulted in reduced beta-catenin expression and inhibited tumor cell growth.
- The study found that lowering beta-catenin led to cell cycle arrest without cell death and significantly reduced tumor growth in animal models, suggesting this method could be a new treatment approach for esophageal cancer.
[Effect of Twist gene on the migration and invasion of gastric carcinoma cells].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
February 2008
Objective: To explore the effect of Twist gene on the migration and invasion of human gastric carcinoma cells.
Methods: MKN28 cells, a human gastric carcinoma cell line, were transfected with PcDNA3.1-Twist plasmid by lipofectamine transfecting technique.
[Expression and significance of PTEN and nm23 in the metastasis of gallbladder cancer].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
February 2005
Objective: To explore the relationship between the expressions of PTEN and the metastasis of the gallbladder cancer.
Methods: The expression of PTEN and nm23 were detected by immunohistochemical staining in 32 cases of gallbladder cancer with metastasis and the staining intensity was scored semi-quantitatively, compared with the cases without metastasis.
Results: The intensity score of PTEN and nm23 in gallbladder cancer with metastasis was 8.
[Pathological analysis of metastatic brain tumors misdiagnosed with MRI].
Hunan Yi Ke Da Xue Xue Bao
June 2002
Objective: To study the pathological basis of metastatic brain tumors misdiagnosed with (MRI).
Methods: Seven cases were analyzed histopathologically and immunohistochemically, compared with the MRI image.
Results: All the tumors were single large noduls.