Lipopolysaccharide promotes cancer cell migration and invasion through METTL3/PI3K/AKT signaling in human cholangiocarcinoma.

Purpose: As a major structural component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) has been detected in the blood circulation and tissues in patients with chronic diseases and cancers, which plays a critical role in the tumor formation and progression. However, the biological role of LPS in human intrahepatic cholangiocarcinoma remains unclear. The aims of this study were to investigate the role of LPS in the malignant progression of intrahepatic cholangiocarcinoma.

Heart failure classification using deep learning to extract spatiotemporal features from ECG.

Background: Heart failure is a syndrome with complex clinical manifestations. Due to increasing population aging, heart failure has become a major medical problem worldwide. In this study, we used the MIMIC-III public database to extract the temporal and spatial characteristics of electrocardiogram (ECG) signals from patients with heart failure.

[Comparative analysis of clinical efficacy between posterior percutaneous endoscopic discectomy and anterior cervical discectomy and fusion in the treatment of cervical spondylotic radiculopathy].

Objective: To explore the clinical efficacy of posterior percutaneous endoscopic discectomy(PPECD) in the treatment of cervical spondylotic radiculopathy.

Methods: A total of 56 patiens with single segment cervical spondylotic radiculopathy from December 2017 to October 2020, were randomly divided into observation group and control group. In observation group, there were 16 males and 11 females, including 8 cases of C, 13 cases of C and 6 cases of C performed posterior percutaneous endoscopic discectomy, aged from 34 to 61 years old with an average of (51.

A Comparison of Co-expression Networks in Silk Gland Reveals the Causes of Silk Yield Increase During Silkworm Domestication.

Long-term domestication and selective breeding have increased the silk yield of the domestic silkworm () by several times the amount of the silk yield of its wild ancestor (). However, little is known about the molecular mechanisms behind the increase in silk yield during domestication. Based on dynamic patterns of functional divergence in the silk gland between domestic and wild silkworms, we found that at early and intermediate stages of silk gland development, the up-regulated genes of the domestic silkworm were mainly involved in DNA integration, nucleic acid binding, and transporter activity, which are related to the division and growth of cells.

An adaptive enhancement method for breast X-ray images based on the nonsubsampled contourlet transform domain and whale optimization algorithm.

We propose a new method for breast X-ray image adaptive enhancement that combines nonsubsampled contourlet transform (NSCT) with the whale optimization algorithm (WOA). First, the mammography X-ray image was processed by histogram equalization to ensure global image contrast. The processed image was then decomposed into three layers in the NSCT domain.

MRI denoising by NeighShrink based on chi-square unbiased risk estimation.

NeighShrink is an efficient image denoising algorithm for the reduction of additive white Gaussian noise. However, it does not perform well in terms of Rician noise removal for MRI (Magnetic Resonance Imaging). Allowing for the characteristics of squared-magnitude MR (Magnetic Resonance) images, which follow a non-central chi-square distribution, the CURE (Chi-Square Unbiased Risk Estimation) is used to determine an optimal threshold for NeighShrink.

[Comparison of early clinical effects between Activ C cervical disc replacement and anterior cervical discectomy and fusion for single-level cervical spondylosis].

Objective: To compare the early clinical effects of Activ C cervical disc replacement (ACDR) and anterior cervical discectomy and fusion (ACDF) in treating single-level cervical spondylosis.

Methods: The clinical data of 76 patients with single-level cervical spondylosis underwent surgery from July 2009 to September 2012 were retrospectively analyzed. Among them, 28 patients were treated with ACDR (ACDR group), including 18 males and 10 females, aged from 32 to 62 years old with an average of (45.

[Long-term assessment of relapse and associated risk factors in chronic hepatitis C patients treated with interferon and ribavirin].

Objective: To investigate viral relapse and the associated risk factors during a long-term follow-up study of chronic hepatitis C (CHC) patients who achieved end-of-treatment response (ETR) after interferon and ribavirin therapy.

Methods: This retrospective study was conducted on 146 CHC patients treated with a combination of ribavirin and pegylated (PEG) interferon-alpha (IFNa) (n=126) or conventional IFNa (n=20) for 24 (hepatitis C virus (HCV) non-genotype 1b) or 48 (HCV genotype 1b) weeks. The main outcome measure was serum HCV RNA load.

Factors influencing inadequate or suboptimal response to adefovir with or without genotypic resistance.

The aim of the study was to explore the factors in inadequate-responders to treatment with adefovir (ADV) with or without genotypic resistance. The reverse-transcriptase (RT) gene of hepatitis B virus (HBV) was sequenced in 161 patients with inadequate-response to ADV and analyzed for HBV genotypes using a phylogenetic approach. Seventy-six patients (47.

[Positive selection analysis of hepatitis C virus genotype 1b envelope 2 gene and its significance during natural chronic infection].

Aim: To elucidate adaptive evolution patterns and perform a positive selection analysis of hepatitis C virus(HCV) genotype 1b envelope 2 gene(E2) during natural chronic infection by a longitudinal study.

Methods: HCV E2 quasispecies profiles were derived from partially sequenced domains of 6 000 bp recombinant clones. Phylogenetic trees for HCV E2 gene were constructed by MEGA software and the specific codons undergoing diversifying positive selection were identified by FEL method.

[Identification of Papaver somni ferum L. and Papaver rhoeas using DSWT-FTIR-RBFNN].

Infrared spectra of Papaver somniferum L. and Papaver rhoeas were obtained directly, quickly and accurately by Fourier transform infrared spectroscopy (FTIR)with OMNI sampler. Discrete stationary wavelet transform was used to extrude local region of infrared spectra of Papaver somniferum L.

[Identification of semen celosiae and semen celosiae cristatae using continuous wavelet transform with FTIR].

Infrared spectra of semen celosiae and semen celosiae cristatae were obtained directly, quickly and accurately by Fourier transform infrared spectroscopy (FTIR) with OMNI sampler. Continuous wavelet transform was used to extrude local region of infrared spectra of semen celosiae and its confusable varieties. The difference of infrared spectra between semen celosiae and semen celosiae cristatae was extruded greatly.

[Application of wavelet transform to infrared analysis].

In the present article the FTIR spectra of the xylems of Smilax glabra Roxb. and its three kinds of counterfeits were obtained by Fourier transform infrared spectroscopy (FTIR) with OMNI-sampler directly, fast and accurately. By adopting wavelet transform analytical method the samples were studied in detail.