Vasoactive intestinal peptide administration after stroke in rats enhances neurogenesis and improves neurological function.

The aim of this study was to investigate the effects of vasoactive intestinal peptide (VIP) on neurogenesis and neurological function after cerebral ischemia. Rats were intracerebroventricular administered with VIP after a 2h middle cerebral artery occlusion (MCAO) and sacrificed at 7, 14 and 28 days after MCAO. Functional outcome was studied with the modified neurological severity score.

Vasoactive intestinal peptide increases VEGF expression to promote proliferation of brain vascular endothelial cells via the cAMP/PKA pathway after ischemic insult in vitro.

Vasoactive intestinal peptide (VIP) enhances angiogenesis in rats with focal cerebral ischemia. In the present study, we investigated the molecular mechanism of the proangiogenic action of VIP using an in vitro ischemic model, in which rat brain microvascular endothelial cells (RBMECs) are subjected to oxygen and glucose deprivation (OGD). Western blotting and immunocytochemistry were carried out to examine the expression of VIP receptors and vascular endothelial growth factor (VEGF) in cultured RBMECs.

[Vasoactive intestinal peptide enhances angiogenesis after focal cerebral ischemia].

Objective: To investigate the effect of vasoactive intestinal peptide (VIP) on angiogenesis after focal cerebral ischemia.

Methods: Focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) for 120 min in adult SD rats with intracerebroventricular VIP administration at the beginning of reperfusion. Immunohistochemistry was performed to assay BrdU immunoreactive endothelial cells, expressions of VEGF, flt-1 and flk-1 in the ischemic zone, and the protein expressions of vascular endothelial growth factor (VEGF) in the brain was measured using Western blotting.

[Vasoactive intestinal peptide attenuates apoptosis and iNOS protein expression induced by cerebral ischemia in the rat].

Objective: To explore the neuroprotective action of vasoactive intestinal peptide (VIP) on ischemia and reperfusion in the rat.

Methods: VIP was given via intracerebroventriclar injection after a 2 hour transient middle cerebral artery occlusion using filament model. The infarct volume was investigated with TTC stain.

[Neuroprotective effects of vasoactive intestinal peptide against rat cerebral ischemia].

Objective: To explore the neuroprotective effect of vasoactive intestinal peptide (VIP) in rat ischemic brain injury.

Methods: VIP was administered via intracerebroventricular injection in SD rats prior to focal cerebral ischemia by intraluminal occlusion of the middle cerebral artery. The infarct volume was assessed with TTC staining, and immunohistochemistry was performed to analyze the S100beta expression in the cerebral tissue, with the serum concentrations of S100beta detected by double-antibody sandwich enzyme-linked immunosorbent assay.