Application of genome tagging technology in elucidating the function of sperm-specific protein 411 (Ssp411).

The genome tagging project (GTP) plays a pivotal role in addressing a critical gap in the understanding of protein functions. Within this framework, we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411 (HA-tagged Ssp411) mouse model. This model is instrumental in probing the expression and function of Ssp411.

[Effect of integral syndrome differentiation acupuncture on the tear film stability in the patient of xerophthalmia].

Objective: To probe into a treatment method for increasing clinical therapeutic effect on xerophthalmia.

Methods: Forty-five cases were randomly divided into acupuncture group 1, acupuncture group 2 and control group. The acupuncture group 1 were treated with acupuncture based on syndrome types, and the acupuncture group 2 with acupuncture at local acupoints, and the control group with dripping of artificial tears.

[Establishment and application of fluorescent quantitative PCR method for detecting human herpes virus type 6].

Objective: To establish fluorescent quantitative PCR method for detecting human herpes virus type 6 (HHV6).

Methods: According to the specific sequence of human herpes virus type 6 genes, the primers and the fluorescent probe (TaqMan) were designed and synthesized. The fragment generated from HHV 6 gene as template was cloned into the pMD18-T vector which was constructed from the pUC 18.

Adenovirus-Mediated th Gene Therapy of Parkinson's Disease in a Experimental Rat Model.

A recombinant adenovirus (AdCMV th) encoding tyrosine hydroxylase (TH) gene with CMV promoter was constructed and propagated. Southern blot analyses was used to identify positive plaques. Virus titer was about 1.