Saponins from improve neuronal mitochondrial injury of aging rats.

Context: is the dried rhizome of C.A. Mey.

Saponins from attenuate cognitive impairment in ageing rats through regulating microglial polarisation and autophagy.

Context: is the dried rhizome of C.A. Mey.

[Effect of total saponins from Panax japonicus on non-alcoholic steatohepatitis by regulating autophagy].

Non-alcoholic steatohepatitis(NASH) was induced by high-sugar and high-fat diet in mice to investigate the intervention effect of total saponins from Panax japonicus(TSPJ) and explore its possible mechanism. Mice were fed with high-sugar and high-fat diet to establish NASH model, and intervened with different doses of TSPJ(15, 45 mg·kg~(-1)). The animals were fed for 26 weeks.

Saponins of Panax japonicus Confer Neuroprotection against Brain Aging through Mitochondrial Related Oxidative Stress and Autophagy in Rats.

Background: Oxidative stress and mitochondrial dysfunction play a vital role in the pathogenesis of brain aging. Saponins from Panax japonicus (SPJ) have attracted much attention for their potential to attenuate age-related oxidative stress as the main ingredient in rhizomes of Panax japonicus.

Objective: This study aimed to investigate the neuroprotective effects of SPJ on natural aging rats as well as the underlying mechanisms regarding oxidative stress and mitochondrial pathway.

[Improved effects of saponins from Panax japonicus on decline of cognitive function in natural aging rats via NLRP3 inflammasome pathway].

The aim of this paper was to investigate the effect of total saponins from Panax japonicus( SPJ) on cognitive decline of natural aging rats and its mechanism. Thirty male SD rats of eighteen month old were randomly divided into three groups: aged group,10 mg·kg~(-1) SPJ-treated group and 30 mg·kg~(-1) SPJ-treated group. The SPJ-treated groups were given SPJ at the dosages of 10 mg·kg~(-1) and 30 mg·kg~(-1),respectively,from the age of 18 to 24 months.

[Triterpene saponins in Panax japonicus and their ~(13)C-NMR spectroscopic characteristics].

Panax japonicus( PJ) is a valuable medicinal plant belonging to the genus Panax of Araliaceae,the recumbent rhizome of which is widely used in clinic therapy,healthcare products and as cosmetic additives with functions of dissipating stasis,reducing swelling,stanching bleeding,and reinforcing deficiency,etc. PJ contains abundant levels of oleanane-and dammarane-type triterpene saponins,which are considered as the material basis for exerting pharmacodynamic action. Based on the previous researches,more than110 triterpene saponins have been reported from PJ.

[Effect of saponins extracted from Panax japonicas on inhibiting myocardial fibrosis by TGF-β1/Smad3 signaling pathway in aging rats].

To investigate the amelioration effect of saponins extracted from Panax japonicas (SPJ) on myocardial fibrosis in natural aging rats and its mechanisms, male SD rats aged 18 months were randomly divided into 3 groups (aging model group, low-dose SPJ group and high-dose SPJ group), with 10 rats in each group. SPJ groups were given SPJ at different doses (10, 60 mg·kg⁻¹·d⁻¹) consecutively for 6 months, meanwhile, aging model group was treated with the equal volume of saline for 6 months until 24 months old. Another 10 rats aged 6 month were used as young control group.

[Protective effect of Wuzi Yanzong recipe on testicular germ cell apoptosis in natural ageing rats through endoplasmic reticulum stress].

To study the protective effects of Wuzi Yanzong recipe on testis germ cell apoptosis in natural ageing rats through endoplasmic reticulum stress (ERS), 16-month-old male SPF grade SD rats were randomly divided into three groups: ageing model group, and low and high-dose Wuzi Yanzong recipe groups (WZ, 1 and 4 g·kg⁻¹), with 10 rats in each group. In addition, 2-month-old SD male rats were used as adult control group. The ageing model group and the adult control group were fed with normal diet for 4 months.

[Protective effects of total saponins of Panax japonicas on non-alcoholic fatty liver disease through regulating expression of miR-199-5p].

To research the effection and probable mechanism for the total saponins of Panax japonicas(TPSJ) in mice on non-alcoholic fatty liver disease. Forty SPF male Kunming mice were randomily divided into four group:control group,NAFLD group, low-dose TPSJ treated group,high-dose TPSJ treated group. High-fatty and high-frutose-diet was applied to eatablish NAFLD model，and TPSJ (100 and 200 mg·kg⁻¹) in feeding were given for the TPSJ groups for 4 weeks.

[Protective effect of Wuzi Yanzong recipe on testicular DNA oxidative damage in natural ageing rats].

To study the protective effects of Wuzi Yanzong recipe on DNA oxidative damage of testis germ cells in natural ageing rats based on Nrf2/HO-1 signaling pathway and base excision repair (BER). In the study, 16-month-old SPF grade male SD rats were randomly divided into three groups, namely ageing model group, and low and high-dose Wuzi Yanzong recipe groups (WZ, 1, 4 g·kg⁻¹). In addition, 2-month-old SD rats were used as adult control group (10 rats in each group).

[Protective effect of Wuzi Yanzong recipe on testicular DNA damage and apoptosis in natural ageing rats].

To study the protective effect of Wuzi Yanzong recipe on testicular DNA damage and apoptosis in natural ageing rats, SPF grade 16-month-old SD male rats were randomly divided into three groups: ageing model group, low and high dose Wuzi Yanzong recipe groups (WZ, 1， 4 g·kg⁻¹). In addition, 2-month-old SD rats were used as adult control group (10 rats in each group). The ageing model group and the adult control group were fed with normal diet for 4 months.

[Protective effects of total saponins of Panax japonicas on HepG2 cell apoptosis induced with palmitic acid].

This study aimed to investigate the molecular mechanism and protective effect of total saponins of Panax japonicas (TSPJ) on HepG2 cells apoptosis induced by palmitic acid (PA).The HepG2 cells were cultured , and divided into five groups: the control group, the model group, the high-dose group (50 mg·L⁻¹), the middle-dose group (25 mg·L⁻¹) and the low-dose group (12.5 mg·L⁻¹).

[Effect of saponins extracted from Panax japonicus on inhibiting cardiomyocyte apoptosis by AMPK/Sirt1/NF-κB signaling pathway in aging rats].

To investigate the effects of saponins extracted from Panax japonicus(SPJ) on cardiomyocyte apoptosis in natural aging rats and explore its underlying mechanisms. SD male rats were randomly divided into four groups: young control group, natural aging group, SPJ low dose group and SPJ high dose group, with 10 rats in each group. The rats in natural aging group, SPJ low and high dose groups were respectively treated with normal saline, SPJ 10 and 60 mg•kg-1•d-1 from the beginning of 18 month-old, 6 days per week for 6 months till 24 month-old.

Saponins from attenuate age-related neuroinflammation regulation of the mitogen-activated protein kinase and nuclear factor kappa B signaling pathways.

Neuroinflammation is recognized as an important pathogenic factor for aging and related cognitive disorders. Mitogen-activated protein kinase and nuclear factor kappa B signaling pathways may mediate neuroinflammation. Saponins from Panax japonicus are the most abundant and bioactive members in rhizomes of Panax japonicus, and show anti-inflammatory activity.

[Protective effect of total saponins of Panax notoginseng combined with total flavonoids of epimedium on D-galactose-incuced senescence of H9c2 cell].

To investigate the protective effect of Panax notoginseng saponins combined with total flavonoids of epimedium on D-gal-induced senescence of H9c2 cells and explore its underlying mechanisms. The 50 mol•L⁻¹ D-gal was used to induce H9c2 cells senescence. Different concentrations of TPNS, TFE, and TPNS combined with TFE were used for 4 hours for pre-treatment.

[Effects of the Saponins from Panax japonicus on Intestinal Epithelial Tight Junction Proteins in Aging Rats].

Objective: To investigate the moderating effects of total saponins of Panax japonicus on intestinal epithelial tight junction proteins in aging rats,and to explore the potential mechanism.

Methods: SD rats were divided into adult group( 6 months),old model group( 24 months),and different doses( 10,30 and 60 mg / kg) of total saponins of Panax japonicus treatment groups. Levels of tight junction proteins( Occludin and ZO-1),anti-oxidative pathway proteins( Nrf2、HO-1 and NQO-1),mitochondrial biogenesis related proteins( Sirt1 and PGC-1α) and p-AMPK in the ileum were determined by immunohistochemistry staining.

[Protective Effect of Wuzi Yanzong Prescription on Apoptosis in Germ Cells of Mice Induced by Cyclophosphamide].

Objective: To investigate the protective effect and mechanism of Wuzi Yanzong prescription on apoptosis in germ cell of adult male mice induced by cyclophosphamide( CTX).

Methods: Male Balb / C mice were randomly divided into normal control group,model group,the low-,medium- and high- dose of Wuzi Yanzong prescription groups( 100 mg / kg,200 mg / kg and 400 mg / kg),with 10 mice in each group. The mice were injected intraperitoneally with CTX( 200 mg / kg) from 4th day,and gave drug once a week,and executed for 4 weeks.

[Protective Effects of Total Saponins from Panax japonicus on H₂O₂Induced Injury via Mitochondia Pathway in SH-SY5Y Cells].

Objective: To investigate the protective mechanism of total saponins from Panax japonicus (SPJ) on H₂O₂ induced injury in SH-SY5Y cells.

Methods: SH-SY5Y cells were divided into three groups: blank control group, model group (600 µmol/L H₂O₂) and drug treatment groups. Different concentrations of SPJ (0.

Chikusetsusaponin V attenuates lipopolysaccharide-induced liver injury in mice.

Chikusetsusaponin V (CsV), a saponin from Panax japonicus, has been reported to inhibit inflammatory responses in lipopolysaccharide (LPS)-induced macrophage cells. However, whether CsV could alleviate LPS-induced liver injury in vivo and the potential mechanisms involved remain unclear. In the present study, we investigated the anti-inflammatory effects of CsV on LPS-induced acute liver injury in mice and further explored the potential mechanisms involved.

[Protective Effect of Total Saponins of Panax japonicus Against H2O2-Induced Oxidative Stress Damage].

Objective: To investigate the protective effects of total saponins of Panax japonicus (TSPJ) on H2O2-induced oxidative stress damage in SH-SY5Y cells, and to explore the underlying mechanism.

Methods: SH-SY5Y cells were incubated with 600 μmol/L H2O2 for 12 h, then treated with various concentrations of TSPJ (0.1, 1, 5 and 20 μg/mL) for 12 h,and then incubated with 600 μmol/ L H2O2 for 12 h.

[Protective Effect of Total Flavonoids of Epimedium on D-galactose-induced Senescence in H9c2 Cells].

Objective: To investigate the protective effect of Total Flavonids of Epimedium (TFE) on D-galactose-induced H9c2 cells senescence and to explore the underlying mechanisms.

Methods: D-galactosed (50 mmol/L) were used to induce the aging of H9c2 cells. Different concentrations of TFE (5,25 and 50 µg/mL) were added into the medium before the use of D-galactosed.

[Study on protective effect of Panax notoginseng total saponins on H9c2 cells senescence against D-galactose].

Objective: To investigate the protective effect of Panax notoginseng Total Saponins (PNTS) on D-galactose-induced H9c2 cell senescence and the underlying mechanism.

Methods: D-galactose was used to cause H9c2 cells senescence. Different concentrations of PNTS (5,25 and 50 μg/mL) were added into medium to protect H9c2 cells.

[Study on protective effect of total saponins of Panax japonicus on LPS-induced RAW264. 7 cell inflammation through NF-kappaB pathway].

Objective: To observe the anti-inflammatory effect of total saponins of Panax japonicus on LPS-induced RAW264. 7 macrophages.

Method: The effect of total saponins of P.

Chikusetsu saponin V attenuates MPP+-induced neurotoxicity in SH-SY5Y cells via regulation of Sirt1/Mn-SOD and GRP78/caspase-12 pathways.

Studies have shown that saponins from Panax japonicus (SPJ) possess neuroprotective effects. However, whether Chikusetsu saponin V (CsV), the most abundant member of SPJ, can exert neuroprotective effects against 1-methyl-4-phenylpyridinium ion (MPP+)-induced cytotoxicity is not known. In this study, we aimed to investigate the neuroprotective effects of CsV on MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells and explore its possible mechanisms.