Publications by authors named "Chang-Chen Hu"
Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with age.
View Article and Find Full Text PDFA previous report has confirmed the existence and clinical significance of vasculogenic mimicry (VM) in glioma. However, its conclusions about the negative clinical significance of VM in glioblastoma are based on a small group of patients and, thus, might be unconvincing. The aim of the present study was to reevaluate the clinical significance of VM in glioblastoma.
View Article and Find Full Text PDFArticle Synopsis
- Immunotoxins show potential as an alternative treatment for brain cancers like gliomas, but they struggle to effectively penetrate tumor tissue.
- This study explores using human bone marrow-derived mesenchymal stem cells (hMSCs) to deliver EphrinA1-PE38, an immunotoxin targeting the EphA2 receptor prevalent in gliomas.
- Results demonstrated that engineered hMSCs can express and release the immunotoxin, leading to effective tumor growth inhibition in a glioma model, highlighting gene therapy's promise for treating these tumors.
Article Synopsis
- Inhibition of tumor neovascularization can significantly impact the growth of solid tumors, as shown in this study exploring the use of VEGF165-PE38 immunotoxin for cancer treatment.
- The study demonstrated that chick chorioallantoic membrane assays and a murine malignant glioma model showed decreased growth of blood vessels and tumor volume when using a plasmid encoding VEGF165-PE38, indicating its antiangiogenic effects.
- Immunohistochemistry analysis revealed reduced CD31 expression and microvessel density in treated groups, supporting the effectiveness of gene therapy to produce VEGF165-PE38 in targeting malignant gliomas.
[Prokaryotic expression, purification of human LINGO-1(aa76-319) and preparation of its polyclonal antibody].
Nan Fang Yi Ke Da Xue Xue Bao
November 2009
Objective: To express and purify the fusion protein of extracellular domain of human Ig domain-containing, neurite outgrowth inhibitor (Nogo) receptor-interacting protein-1 (LINGO-1(aa76-319)) in prokaryotic cells and prepare the rabbit anti-LINGO-1 polyclonal antibody (pAb).
Methods: The 732 bp DNA sequence of hLINGO-1(aa76-319) was obtained from pCMV-SPORT6 by PCR and inserted into pET30a(+) plasmid to construct the prokaryotic expression plasmid pET30a(+)-hLINGO-1(aa76-319), which was subsequently transformed into E.coli.
Bone marrow stroma cells-derived neural stem cells (BMSCs-D-NSCs) transplantation is a promising strategy for the treatment of nervous system disorders. The development of a non-invasive method to follow the fate of BMSCs-D-NSCs in vivo is very important for the future application of this treatment. In this paper, we show for the first time, that BMSCs-D-NSCs from rhesus monkeys can be labeled in vitro with the superparamagnetic iron oxide (SPIO) contrast agent Feridex and Poly-L-lysine (PLL) without affecting morphology, cell cycle, telomerase activity, proliferation and differentiation ability of the labeled cells.
View Article and Find Full Text PDFHuman mesenchymal stem cells-like cells (hMSCs-like cells) were used as a tumor treatment platform for the systemic delivery of immunotoxin genes. VEGF165-PE38 recombinant immunotoxin served as the model system. hMSCs-like cells were isolated, expanded, and electroporated with the pIRES2-VEGF165PE38-EGFP plasmid.
View Article and Find Full Text PDF